FlyBase Allele Report: Dmel\Gprk2[RNAi.UAS.cMa]

General Information

Symbol

Dmel\Gprk2^RNAi.UAS.cMa

Species

D. melanogaster

Name

FlyBase ID

FBal0244618

Feature type

allele

Associated gene

Dmel\Gprk2

Associated Insertion(s)

Carried in Construct

P{UAS-Gprk2.RNAi.M}

Key Links

Transgenic product class

RNAi_reagent

(Molnar et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Molnar et al., 2007)

Mutagen

in vitro construct

(Molnar et al., 2007)

Progenitor genotype

Carried in construct

P{UAS-Gprk2.RNAi.M}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of a 500 bp fragment encoding the Gprk2 C-terminus.

(Molnar et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Gprk2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^MD-638

(Molnar et al., 2007)

anterior crossvein, with Scer\GAL4^ptc-559.1

(Molnar et al., 2007)

wing vein L3, with Scer\GAL4^MD-638

(Molnar et al., 2007)

wing vein L3, with Scer\GAL4^ptc-559.1

(Molnar et al., 2007)

wing vein L4, with Scer\GAL4^MD-638

(Molnar et al., 2007)

wing vein L4, with Scer\GAL4^ptc-559.1

(Molnar et al., 2007)

Detailed Description

Statement

Reference

Expression of Gprk2^{dsRNA.Scer\UAS} in the wing under the control of Scer\GAL4^MD-638 results in a reduction of the L3/L4 intervein, fusion of the L3 and L4 veins, and in a small proportion of wings, loss of the L3 and L4 veins.

Expression of Gprk2^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ptc-559.1 in anterior cells close to the A/P compartment boundary results in disruption to wing veins L3 and L4, and the L3/L4 intervein.

Wing veins are normal in flies expressing Gprk2^{dsRNA.Scer\UAS} in the posterior compartment of the wing under the control of Scer\GAL4^hh-Gal4.

(Molnar et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Gprk2^RNAi.UAS.cMa, Scer\GAL4^MD-638 has wing phenotype, enhanceable by smo^{ΔC2.UAS.Tag:FLAG}, Scer\GAL4^MD-638

(Molnar et al., 2007)

Enhancer of

Statement

Reference

Gprk2^RNAi.UAS.cMa, Scer\GAL4^MD-638 is an enhancer of wing vein L3 phenotype of Scer\GAL4^MD-638, hh^NIG.4637R

(Molnar et al., 2007)

Gprk2^RNAi.UAS.cMa, Scer\GAL4^MD-638 is an enhancer of wing vein L4 phenotype of Scer\GAL4^MD-638, hh^NIG.4637R

(Molnar et al., 2007)

Gprk2^RNAi.UAS.cMa, Scer\GAL4^MD-638 is an enhancer of anterior crossvein phenotype of Scer\GAL4^MD-638, hh^NIG.4637R

(Molnar et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of smo^{ΔC2.Scer\UAS.T:Zzzz\FLAG} and Gprk2^{dsRNA.Scer\UAS} in the wing under the control of Scer\GAL4^MD-638 results in a severe wing phenotype.

Expression of Gprk2^{dsRNA.Scer\UAS} in flies expressing hh^NIG.4637R in the wing under the control of Scer\GAL4^MD-638 strongly enhances the wing vein phenotype.

(Molnar et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

44610

w^*; P{UAS-Gprk2.RNAi.M}2; P{UAS-Su(fu).H}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Gprk2^RNAi.UAS.cMa

Gprk2^{dsRNA.Scer\UAS}

Gprk2^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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