Open Close
General Information
Symbol
Ecol\lexAfru.P1
Species
E. coli
Name
FlyBase ID
FBal0244777
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
fruLexA, fru-LexA, fruP1.LexA, frulex, fruP1.LexA
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Sequence encoding a lexA::VP16 driver has been inserted at the first codon of the fru[M] open reading frame. This allows for expression of the lexA::VP16 driver in the same pattern as transcripts from the fru[M] (P1) promoter, which is transcribed in both males and females.

Insertion components
TI{lexA::VP16}fruP1.LexA
Product class / Tool use(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Ecol\lexAfru.P1.T:Hsim\VP16 drives expression in chemosensory neurons in the male forelegs. Most of these chemosensory neurons labeled with Ecol\lexAfru.P1.T:Hsim\VP16 also express Scer\GAL4ppk23.2.6.

Ecol\lexAfru.P1.T:Hsim\VP16 drives expression in mechanosensory neurons in the taste bristles of the proboscis. In the legs, Ecol\lexAfru.P1.T:Hsim\VP16 is detected in more then one gustatory receptor neuron per bristle, with these cells also expressing Scer\GAL4ppk23.2.695. Ecol\lexAfru.P1.T:Hsim\VP16 positive neurons do not overlap with expression of Scer\GAL4Gr64f.9.7 which labels the sugar-responsive neurons, candidate pheromone receptors Scer\GAL4Gr32a.3.776 and Scer\GAL4Gr68a.PB, or with Scer\GAL4Gr66a.3.153 which labels bitter-responsive neurons.

Ecol\lexAfru.P1.T:Hsim\VP16 drives expression in 2 clusters of neurons in the adult female genital tract, which co-express Avic\GFPEGFP.ppk.1 : one cluster of 1 neuron in the lateral oviduct and one cluster of 3 neurons in the uterus (which also express Scer\GAL4dsx.KI).

Ecol\lexAfru.P1.T:Hsim\VP16 drives expression in many neurons in the adult brain, including in the mushroom body alpha, beta, gamma, alpha', beta' lobes. In the antennal lobe, Ecol\lexAfru.P1.T:Hsim\VP16 labels the antennal lobe glomeruli DA1 and VA1v.

Ecol\lexAfru.P1 drives expression in in a subset of gustatory neurons of prothoracic tarsal segments 3-5 at 18-20 hours APF; no expression is observed prothoracic tarsal segments 1-2 at this stage. By 24-28 hours, expression is driven in gustatory neurons of all prothoracic tarsal segments. At the earlier timepoint, expression is observed in both male and female prothoracic tarsi, while expression at 28 hours APF is much more pronounced in male than female tarsi.

 
Marker for
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Inhibition (using Ctet\TeTxLCFRT.stop.UAS with Scer\FLP1L.8xlexAop) of neurons expressing Ecol\lexAfru.P1 and any of Scer\GAL4GMR21B12, Scer\GAL4GMR22B11, Scer\GAL4GMR49F09, Scer\GAL4GMR72E10, or Scer\GAL4GMR46F09 significantly reduces male-male courtship chaining responses to pulse song, compared to controls.

Optogenetic activation (using Cnoc\ChR1Cs.20xUAS.FRT.Venus with Scer\FLP1L.8xlexAop and activating with 655 nm (red) light) of neurons expressing Ecol\lexAfru.P1 and any of Scer\GAL4GMR72E10, Scer\GAL4GMR46F09, but not Scer\GAL4GMR22B11 or Scer\GAL4GMR49F09, significantly increases male-male courtship chaining, compared to controls.

Thermogenetic activation of Tk-GAL4[Fru[M] neurons (neurons labelled by both Scer\GAL4Tk and Ecol\lexAfru.P1.T:Hsim\VP16) increases male-male aggression compared to controls. Ecol\lexAfru.P1.T:Hsim\VP16 drives expression of Scer\FLP1L.8xEcol\lexAop, which results in excision of the Scer\FRT cassette from TrpA1Scer\FRT.stop.Scer\UAS.T:Disc\RFP-mCherry enabling these cells to express TrpA1 under the control of Scer\GAL4Tk when the flies are shifted to the permissive temperature. Activation of Tk-GAL4[Fru[M] neurons does not induce aggression of male flies towards females, and they copulate at a rate comparable to that observed in controls. Copulation latency and the average duration of copulation do not differ from controls. There is no change in courtship behavior; the frequency of unilateral wing extension or "singing" towards mated females is unchanged.

Thermogenetic activation of Tk-GAL4[Fru[M] neurons (neurons labelled by both Scer\GAL4Tk and Ecol\lexAfru.P1.T:Hsim\VP16) increases male-male aggression compared to controls. Ecol\lexAfru.P1.T:Hsim\VP16 drives expression of Scer\FLP1L.8xEcol\lexAop, which results in excision of the Scer\FRT cassette from TrpA1Scer\FRT.stop.Scer\UAS.T:Hsap\MYC enabling these cells to express TrpA1 under the control of Scer\GAL4Tk when the flies are shifted to the permissive temperature. There is no change in male-male courtship behavior; the frequency of unilateral wing extension or "singing" towards other males is unchanged.

Silencing of Tk-GAL4[Fru[M] neurons (neurons labelled by both Scer\GAL4Tk and Ecol\lexAfru.P1.T:Hsim\VP16) reduces levels of male-male aggression compared to controls, although the level of baseline aggression between different controls varies. Ecol\lexAfru.P1.T:Hsim\VP16 drives expression of Scer\FLP1L.8xEcol\lexAop, which results in excision of the Scer\FRT cassette from Hsap\KCNJ2Scer\FRT.Stop.Scer\UAS.T:Avic\GFP-EGFP enabling these cells to express Hsap\KCNJ2 under the control of Scer\GAL4Tk. The males copulate at a rate comparable to that observed in controls. Copulation latency and the average duration of copulation do not differ from controls. There is no change in courtship behavior; the frequency of unilateral wing extension or "singing" towards mated females is unchanged. Silencing of Tk-GAL4[Fru[M] neurons does not consistently influence the frequency of unilateral wing extensions towards other males in comparison with control strains.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (13)
References (30)