FB2025_01 , released February 20, 2025
Allele: Dmel\Pnglex18
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General Information
Symbol
Dmel\Pnglex18
Species
D. melanogaster
Name
FlyBase ID
FBal0244827
Feature type
allele
Associated gene
Dmel\Pngl
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Delta2-3 transposase
(Funakoshi et al., 2010)
Progenitor genotype
P{SUPor-P}PnglKG05548
(Funakoshi et al., 2010)
Cytology
Description

Imprecise excision of the progenitor insertion, resulting in a deletion that removes part of the Pngl transcription unit. The deletion extends from just upstream of the shorter Pngl-RA transcript (within the second intron of the larger Pngl-RB transcript) into the protein coding region, and is predicted to express an in-frame truncated protein.

(Funakoshi et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Pnglex18 homozygotes are nearly lethal and the rare escapers are sterile. The Pnglex18 homozygous larval midgut exhibits severely shortened gastric caeca and a reduction of the acid zone, as compared to controls. Likewise, Pnglex18/Df(2R)ED1484 transheterozygotes are nearly lethal and their larval midguts exhibit decreased acid zone, as compared to heterozygous controls.

      (Galeone et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Pnglex18 has lethal phenotype, non-suppressible by Hsap\NGLY1ΔR402.UAS/Scer\GAL4Act5C.PI

      (Galeone et al., 2017)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      The near lethality of Pnglex18 homozygotes is rescued by the expression of Hsap\NGLY1Scer\UAS.cGa, but not of Hsap\NGLY1ΔR402.Scer\UAS, under the control of Scer\GAL4Act5C.PI.

      (Galeone et al., 2017)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Png1ex18
      (Na et al., 2022)
      Pnglex18
      (Han et al., 2020, Galeone et al., 2017, Funakoshi et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (4)