FlyBase Allele Report: Dmel\Nuak[JF02162]

FB2025_01 , released February 20, 2025

FB2025_01 , released February 20, 2025

Allele: Dmel\Nuak^JF02162

General Information

Symbol

Dmel\Nuak^JF02162

Species

D. melanogaster

Name

FlyBase ID

FBal0244853

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02162}

Key Links

Genomic Maps

Transgenic product class

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.JF02162}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Also carries

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body size | P-stage, with Scer\GAL4^da.PU

(Brooks et al., 2020)

abnormal body size | P-stage, with Scer\GAL4^how-24B

(Brooks et al., 2020)

abnormal body size | P-stage, with Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^Mef2.PR

(Brooks et al., 2020)

decreased rate of adult locomotory behavior, with Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

decreased size | adult stage, with Scer\GAL4^en-e16E

(Pueyo et al., 2023)

increased body size | P-stage, with Scer\GAL4^Mef2.PR

(Zhao et al., 2023)

short lived, with Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

visible | adult stage, with Scer\GAL4^en-e16E

(Pueyo et al., 2023)

Phenotype Manifest In

actin filament | adult stage, with Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

embryonic/larval hypodermal muscle cell | larval stage, with Scer\GAL4^Mef2.PR

(Brooks et al., 2020)

endosome | larval stage, with Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

indirect flight muscle | adult stage, with Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

larval somatic muscle | larval stage, with Scer\GAL4^C57

(Zhao et al., 2023)

larval somatic muscle | larval stage, with Scer\GAL4^Mef2.PR

(Zhao et al., 2023)

larval somatic muscle | larval stage, with Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

lysosome | larval stage, with Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

muscle cell of ventral longitudinal muscle 3 | larval stage, with Scer\GAL4^Mef2.PR

(Brooks et al., 2020)

sarcomere | increased number | larval stage, with Scer\GAL4^Mef2.PR

(Brooks et al., 2020)

sarcomere | larval stage, with Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

skeletal muscle myofibril | adult stage, with Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

wing posterior compartment, with Scer\GAL4^en-e16E

(Pueyo et al., 2023)

Detailed Description

Statement

Reference

Expression of Nuak^JF02162 under the control of Scer\GAL4^Mef2.PR leads to thinner or detached body wall muscles with regions devoid of F-actin labeled myofibrillar material, and reduced locomotion in third instar larvae. It also leads to an increase in sarcomere number in larval ventral longitudinal muscle 3. The severity of these muscle phenotypes are increased upon starvation.

Expression of Nuak^JF02162 under the control of Scer\GAL4^da.PU or Scer\GAL4^how-24B leads to elongated pupae.

(Brooks et al., 2020)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Nuak^JF02162, Scer\GAL4^Mef2.PU has abnormal body size | P-stage phenotype, enhanceable by NdufA3^UAS.FL, Scer\GAL4^Mef2.PU

(Pueyo et al., 2023)

Phenotype Manifest In

Enhanced by

Statement

Reference

Nuak^JF02162, Scer\GAL4^Mef2.PR has larval somatic muscle cell | larval stage phenotype, enhanceable by stv¹/stv[+]

(Brooks et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

31885

y¹ v¹; P{TRiP.JF02162}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG11870^JF02162

CG43143^JF02162

Nuak1^JF02162

Nuak^JF02162

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections