FB2025_01 , released February 20, 2025
Allele: Dmel\wupAJF02172
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General Information
Symbol
Dmel\wupAJF02172
Species
D. melanogaster
Name
FlyBase ID
FBal0244861
Feature type
allele
Associated gene
Dmel\wupA
Associated Insertion(s)
Carried in Construct
P{TRiP.JF02172}
Key Links
Genomic Maps

Transgenic product class
RNAi_reagent
(Perkins et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Perkins et al., 2009)
Mutagen
in vitro construct
(Perkins et al., 2009)
Progenitor genotype
Carried in construct
P{TRiP.JF02172}
Cytology
Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
dsRNA-JF02172
Also carries
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  adenocarcinoma
      CEA with l(2)glGD4047, Ras85DV12.cUa.UAS
      (Casas-Tintó et al., 2016)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      ameliorates  adenocarcinoma
      modeled by l(2)glGD4047, Ras85DV12.cUa.UAS
      (Casas-Tintó et al., 2016)
      exacerbates  cancer
      modeled by RbfHMS03004
      (Parkhitko et al., 2021)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Somatic clones expressing wupAJF02172 under the control of Scer\GAL4Act.PU in wandering third instar larval wing discs present a significant and progressive decrease in the relative clonal area, as it is smaller at 72h than at 48h after clonal induction, present significant apoptosis, and present recruitment of hemocytes to clonal cells, as compared to control clones.

      The expression of wupAJF02172 under the control of Scer\GAL4en.PU leads to increased apoptosis in the posterior compartment in wandering third instar larval wing discs, as compared to controls.

      (Casas-Tintó et al., 2016)

      Walking and jumping behaviors are unaffected in flies expressing wupAJF02172 under the control of Scer\GAL4Hk-UH3 (limited to the late stages of development using Scer\GAL80ts.αTub84B). Abnormalities are seen in the indirect flight muscle fascicles compared to the wild type and sarcomere organisation is disrupted. This becomes more severe when wupAJF02172 is co-expressed with Dcr-2.

      (Singh et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Enhancer of
      Suppressor of
      Other
      Phenotype Manifest In
      Suppressed by
      Enhancer of
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The co-expression of either fweKK109356, dppScer\UAS.cUa.T:Avic\GFP, SPARCScer\UAS.cPa or bskDN.Scer\UAS suppresses the decreased relative clonal area of somatic clones expressing wupAJF02172 under the control of Scer\GAL4Act.PU in wandering third instar larval wing discs; the co-expression of fweKK109356 or SPARCScer\UAS.cPa also suppress the apoptosis observed in these clones.

      The co-expression of SPARCScer\UAS.cPa suppresses the increased apoptosis observed in the posterior compartment of wandering third instar larval wing discs expressing wupAJF02172 under the control of Scer\GAL4en.PU.

      The induction of somatic clones co-expressing l(2)glGD4047 and Ras85DV12.cUa.Scer\UAS under the control of Scer\GAL4Act.PU during second instar larval stage results in the accumulation of clonal cells in the wandering third instar larval fat body, which is enhanced by the additional co-expression of wupAJF02172; the induction of these clones also results in lethality before adulthood, which is partially rescued by the additional co-expression of wupAJF02172, although the surviving adults display a shorter life-span as compared to wild-type controls.

      In wandering third instar larvae, somatic clones co-expressing l(2)glGD4047 and Ras85DV12.cUa.Scer\UAS under the control of Scer\GAL4Act.PU in both eye discs and wing discs exhibit a significant increase in the relative clonal area and hyperplasia as compared to control clones; wing disc clone cells localize both basally and apically in the disc. All of these phenotypes are suppressed by the additional co-expression of wupAJF02172, even leading to a significant decrease in the wing disc clonal area as compared to control clones.

      (Casas-Tintó et al., 2016)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      wupAJF02172 is rescued by Scer\GAL4Act.PU/wupAf06492

      (Casas-Tintó et al., 2016)
      Comments

      The co-expression of wupAf06492 rescues the progressive decrease in the relative area of somatic clones expressing wupAJF02172 under the control of Scer\GAL4Act.PU in wandering third instar larval wing discs, even leading to a significant increase in the relative clonal area, as compared to control clones.

      (Casas-Tintó et al., 2016)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      wupAJF02172
      Name Synonyms
      Secondary FlyBase IDs
        References (5)