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General Information
Symbol
Hsap\LRRK2Y1699C.UAS
Species
H. sapiens
Name
FlyBase ID
FBal0244981
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a mutant form of Hsap\LRRK2, which contains the amino acid replacement Y1699C.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Axonal transport of mitochondria is disrupted in larval motor neurons expressing Hsap\LRRK2Y1699C.Scer\UAS under the control of Scer\GAL4D42.

Climbing and flight ability are reduced compared to controls in flies expressing Hsap\LRRK2Y1699C.Scer\UAS under the control of Scer\GAL4D42.

Expression of Hsap\LRRK2Y1699C.Scer\UAS driven by Scer\GAL4Ddc.PU causes significant locomotor deficits (reduced climbing ability) in 5 or 10 (but not 20, when all flies including controls show age-dependent locomotor deficits) day old flies, compared to controls.

50 day old adults expressing Hsap\LRRK2Y1699C.Scer\UAS under the control of Scer\GAL4ple.PF at room temperature show loss of dopaminergic neurons in the dorsolateral posterior protocerebral cluster (PPL1) of the brain compared to control flies of the same age. A similar loss of neurons is seen in 20 day old adults expressing Hsap\LRRK2Y1699C.Scer\UAS under the control of Scer\GAL4ple.PF at 29[o]C.

Expression of Hsap\LRRK2Y1699C.Scer\UAS under the control of Scer\GAL4da.G32 at room temperature significantly extends the basal lifespan of flies compared to controls, while expression of Hsap\LRRK2Y1699C.Scer\UAS under the control of Scer\GAL4elav.PU at room temperature or at 29[o]C has no significant effect on basal lifespan.

Flies expressing Hsap\LRRK2Y1699C.Scer\UAS under the control of Scer\GAL4da.G32 at room temperature show a greater sensitivity to chronic exposure to low levels of rotenone then control flies.

Flies expressing Hsap\LRRK2Y1699C.Scer\UAS under the control of Scer\GAL4GMR.PF at 29[o]C show loss of pigmentation in the eyes. Significantly more black lesions are seen in the mutant eyes than in controls. Ultrastructurally, severe disruption of the regular trapezoidal arrangement of the photoreceptor cells is seen. The cell lattice between photoreceptor cell arrays of different ommatidia is completely absent and the ommatidia are sometimes fused together. Large holes are often seen in the eye sections.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Co-expression of either HDAC6KK100215 or Sirt2GD11528 suppresses the defects in axonal transport of mitochondria seen in larval motor neurons expressing Hsap\LRRK2Y1699C.Scer\UAS under the control of Scer\GAL4D42.

Co-expression of either HDAC6KK100215 or Sirt2GD11528 significantly rescues the defects in flight ability seen in flies expressing Hsap\LRRK2Y1699C.Scer\UAS under the control of Scer\GAL4D42.

Co-expression of Vps35EY14200 significantly suppresses locomotor deficits in flies with expression of Hsap\LRRK2Y1699C.Scer\UAS driven by Scer\GAL4Ddc.PU.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Hsap\LRRK2Y1699C.Scer\UAS
Hsap\LRRK2Y1699C.UAS
Name Synonyms
Secondary FlyBase IDs
    References (4)