FB2025_01 , released February 20, 2025
Allele: Scer\GAL4NP2297
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General Information
Symbol
Scer\GAL4NP2297
Species
S. cerevisiae
Name
FlyBase ID
FBal0245026
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
P{GawB}NP2297
Carried in Construct
Also Known As
NP2297
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Associated Insertion(s)
    P{GawB}NP2297
    Cytology
    Description
    Allele components
    Component
    Use(s)
    Inserted element
    P{GawB}
    Encoded product / tool
    GAL4
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        distribution deduced from reporter (Gal4 UAS)
        Stage
        Tissue/Position (including subcellular localization)
        Reference
        adult stage
        mushroom body medial lobe arborizing neuron 1
        (Flybrain Neuron Database et al., 2018.6.28, Shuai et al., 2015, Tanaka et al., 2008)
        mushroom body gamma lobe slice 5
        (Shuai et al., 2015, Tanaka et al., 2008)
        mushroom body beta' lobe slice 2
        (Shuai et al., 2015, Tanaka et al., 2008)
        mushroom body beta' anterior-posterior layer
        (Tanaka et al., 2008)
        mushroom body beta lobe surface layer
        (Tanaka et al., 2008)
        mushroom body beta lobe posterior layer
        (Tanaka et al., 2008)
        mushroom body beta lobe slice 2
        (Shuai et al., 2015, Tanaka et al., 2008)
        mushroom body medial lobe arborizing neuron 7
        (Flybrain Neuron Database et al., 2018.6.28, Shuai et al., 2015, Tanaka et al., 2008)
        mushroom body output neuron 22
        (Flybrain Neuron Database et al., 2018.6.28, Shuai et al., 2015, Tanaka et al., 2008)
        calyx of adult mushroom body
        (Tanaka et al., 2008)
        pedunculus of adult mushroom body
        (Tanaka et al., 2008)
        adult antennal lobe projection neuron DA2 lPN
        (Tanaka et al., 2012)
        adult uniglomerular antennal lobe projection neuron | subset
        (Tanaka et al., 2012)
        adult antennal lobe projection neuron VM4 adPN
        (Tanaka et al., 2012)
        adult multiglomerular antennal lobe projection neuron | subset
        (Tanaka et al., 2012)
        adult brain | restricted
        (Shuai et al., 2015)
        mushroom body extrinsic neuron
        (Shuai et al., 2015)
        mushroom body beta lobe slice 1
        (Shuai et al., 2015)
        Additional Information
        Statement
        Reference

        ScerGAL4NP2297 drives expression in mushroom body extrinsic neurons, including mushroom body medial lobe arborizing neuron 1, mushroom body medial lobe arborizing neuron 7 and mushroom body calyx-pedunculus arborizing neuron 1, that innervate mushroom body gamma lobe slice, mushroom body beta lobe slices 1 and 2, and mushroom body beta' lobe slice 2.

        (Shuai et al., 2015)

        ScerGAL4NP2297 drives expression in 7 uniglomerular projection neurons. Around 2 cell bodies are located in the anterio-dorsal part of the antennal lobe around 5 cell bodies can be found in the lateral part. These neurons project to three glomeruli VC3m. Labeling is also detected in one cell body in the adult subesophageal zone. This neuron projects to glomeruli D, DA2, DL5, DM3, DM6, V, VA6, VC1, VM7v, DA1, DA3, DA4l, DA4m, DC1, DC3, VA1s, VA1v, DL2v, VL1 VC3l, VL2a, VP4, VP2, VP3 and DC4.

        (Tanaka et al., 2012)

        Arborisation within the calyx and pedunculus is due to the mushroom body calyx-pedunculus arborizing neuron 1.

        (Tanaka et al., 2008)
         
        Marker for
        mushroom body medial lobe arborizing neuron 7
        (Tanaka et al., 2008)
        mushroom body output neuron 22
        (Tanaka et al., 2008)
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        Inhibition (using shi1.UAS at 33[o]C) of Scer\GAL4NP2297 cells in adults during testing does not affect medium term (3 h) aversive olfactory memory, compared to controls.

        (Bouzaiane et al., 2015)

        Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4NP2297 neurons, in combination with a Gal80[ts] transgene to restrict expression to 1 day prior to training and testing, does not affect 3 hour aversive olfactory memory, compared to controls.

        (Shuai et al., 2015)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Phenotype Manifest In
        Additional Comments
        Genetic Interactions
        Statement
        Reference
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Comments
        Images (0)
        Mutant
        Wild-type
        Stocks (1)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (1)
        Reported As
        Symbol Synonym
        Scer\GAL4NP2297
        Name Synonyms
        Secondary FlyBase IDs
          References (6)