FB2025_01 , released February 20, 2025
Allele: Scer\GAL4NP3253
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General Information
Symbol
Scer\GAL4NP3253
Species
S. cerevisiae
Name
FlyBase ID
FBal0245068
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
P{GawB}ptcNP3253
Carried in Construct
Also Known As
NP3253, NP3253-GAL4
Genomic Maps

Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Progenitor genotype
    Associated Insertion(s)
    P{GawB}ptcNP3253
    Cytology
    Description
    Allele components
    Component
    Use(s)
    Inserted element
    P{GawB}
    Encoded product / tool
    GAL4
    Mutations Mapped to the Genome
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        distribution deduced from reporter (Gal4 UAS)
        Stage
        Tissue/Position (including subcellular localization)
        Reference
        adult stage
        transverse antennal lobe tract projection neuron
        (Flybrain Neuron Database et al., 2018.6.28, Tanaka et al., 2008)
        calyx of adult mushroom body
        (Tanaka et al., 2008)
        pedunculus of adult mushroom body
        (Tanaka et al., 2008)
        transverse antennal lobe tract 2 projection neuron 1
        (Tanaka et al., 2012)
        antennal lobe hub | restricted
        (Tanaka et al., 2012)
        adult brain | restricted
        (Kenmoku et al., 2016)
        adult proventriculus | restricted
        (Kenmoku et al., 2016)
        adult anterior midgut | restricted
        (Kenmoku et al., 2016)
        adult posterior midgut | restricted
        (Kenmoku et al., 2016)
        adult salivary gland
        (Kenmoku et al., 2016)
        Additional Information
        Statement
        Reference

        ScerGAL4ptc-NP3253 drives expression in two adult multiglomerular antennal lobe projection neurons. Their cell bodies are located in the lateral part of the antennal lobe and they innervate antennal lobe glomeruli DL5, DM1, DM2, DM3, DM4, DM6, V, VA2, VC4, VC1, VM7d, VM7v, DC1, DL2v, DP1l, VC3l, VL1, DP1m, VP1, VP2, VP3 and the antennal lobe hub.

        (Tanaka et al., 2012)
         
        Marker for
        transverse antennal lobe tract projection neuron
        (Tanaka et al., 2008)
        Reflects expression of
        ptc
        (Tanaka et al., 2008)
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        Inhibition (using Hsap\KCNJ2UAS.EGFP) of Scer\GAL4NP3253 cells, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, results in reduced survival rates following infection with Ecc15, compared to controls; restricting the number of inhibited cells (using Scer\GAL80elav.PY) reduces, but does not eliminate, this reduction in survival rate; unlike controls, Ecc15 cells are seen in the whole body of the fly following oral infection; non-infected flies have significantly increased peritrophic matrix permeability, significantly increased stem cell proliferation in the midgut and shorter lifespan, compared to controls; there is a flattening of part of the proventriculus, significantly increased proventriculus lumen and anterior midgut cross-sectional areas, with no obvious change in the number or shape of midgut epithelial cells, compared to controls; there is no effect on feeding or excretion rate, compared to controls.

        Thermogenetic activation (using TrpA1UAS.(B).cKa at 30[o]C) of Scer\GAL4NP3253 cells does not affect survival rate of adults following Ecc15 infection, compared to controls.

        (Kenmoku et al., 2016)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Suppressed by
        Phenotype Manifest In
        NOT Enhanced by
        Statement
        Reference

        Hsap\KCNJ2UAS.EGFP, Scer\GAL4NP3253 has adult anterior midgut phenotype, non-enhanceable by upd3Δ

        (Kenmoku et al., 2016)
        Suppressed by
        Statement
        Reference

        Hsap\KCNJ2UAS.EGFP, Scer\GAL4NP3253 has intestinal stem cell phenotype, suppressible | partially by upd3Δ

        (Kenmoku et al., 2016)
        NOT suppressed by
        Statement
        Reference

        Hsap\KCNJ2UAS.EGFP, Scer\GAL4NP3253 has adult anterior midgut phenotype, non-suppressible by upd3Δ

        (Kenmoku et al., 2016)
        Additional Comments
        Genetic Interactions
        Statement
        Reference
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Comments
        Images (0)
        Mutant
        Wild-type
        Stocks (1)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (2)
        Reported As
        Symbol Synonym
        Scer\GAL4NP3253
        Scer\GAL4ptc-NP3253
        Name Synonyms
        Secondary FlyBase IDs
          References (8)