FlyBase Allele Report: Dmel\Pex10[10]

General Information

Symbol

Dmel\Pex10¹⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0245130

Feature type

allele

Associated gene

Dmel\Pex10

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Chen et al., 2010)

Progenitor genotype

Cytology

Description

Imprecise excision of P{EPgy2}CG12099^EY23523 generates a deletion in pex10 that removed part of exon 1, the whole of exon 2 and part of exon 3 of pex10.

(Chen et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of peroxisomal disease

CEA

(Chen et al., 2010)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis

(Chen et al., 2010)

abnormal developmental rate

(Chen et al., 2010)

abnormal meiotic cell cycle

(Chen et al., 2010)

female semi-fertile

(Chen et al., 2010)

male sterile

(Chen et al., 2010)

viable

(Chen et al., 2010)

Phenotype Manifest In

spermatid

(Chen et al., 2010)

testis

(Chen et al., 2010)

Detailed Description

Statement

Reference

pex10¹⁰ mutants exhibit reduced female fertility and male sterility. pex10¹⁰ mutant testes contain many spermatocytes and some 'cotton ball'-like spheres but completely lack elongated spermatid bundles and mature sperm. The mutant testes appear much thinner and longer on the seventh day after eclosion than they do immediately after eclosion.

pex10¹⁰ mutants display growth defects, with mutants exhibiting a 2-day delay in eclosion.

pex10¹⁰ mutant testes do not contain hardly any normal mature spermatocytes. Instead, only a few fused spermatocytes with condensed chromosomes are found. in addition, there are more spermatocytes in pex10¹⁰ mutants than wild-type. In pex10¹⁰ mutants, cysts of 16 spermatocytes without cell-cell boundaries and with large round nuclei are present. This suggests that the spermatocyte growth stage is defective. The majority of spermatocytes do not mature, and some are fused. the fused spermatocytes are able to continue develop but exhibit profound defects in spermatid differentiation. Only a few round spermatids are found compared with wild-type and they often exhibit four or more nuclei associated with a single enlarged mitochondrial derivative, indicating a failure of cytokinesis during meiotic division. Chromosome segregation appears unaffected. Unlike wild-type testes, which have very long spermatid bundles, the few spermatids in pex10¹⁰ mutants, presumably from a cyst, form 'cotton ball'-like spheres. Thus pex10¹⁰ mutant spermatids can initiate the extension of sperm tails, but this process is abnormal.

pex10¹⁰ mutant spermatocytes go through G1/S phase normally but do not reach the G2/M transition.

Peroxisomal membrane protein targeting and peroxisomal structures appear quite normal in pex10¹⁰ mutants.

Growing pex10¹⁰ mutants on medium to which 2.5% VLCFA has been added results in a significant enhancement of the pex10¹⁰ testis phenotype compared to pex10⁷ flies grown on non-supplemented food. In contrast, adding 2.5% LCFA to the food does not enhance the testis phenotype.

(Chen et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Pex10¹⁰ has male sterile phenotype, non-suppressible by Scer\GAL4^nanos.PG/Pex2^UAS.cCa

(Chen et al., 2010)

Pex10¹⁰ has abnormal developmental rate phenotype, non-suppressible by Scer\GAL4^nanos.PG/Pex2^UAS.cCa

(Chen et al., 2010)

Pex10¹⁰ has abnormal cytokinesis phenotype, non-suppressible by Scer\GAL4^nanos.PG/Pex2^UAS.cCa

(Chen et al., 2010)

Pex10¹⁰ has abnormal meiotic cell cycle phenotype, non-suppressible by Scer\GAL4^nanos.PG/Pex2^UAS.cCa

(Chen et al., 2010)

Pex10¹⁰ has male sterile phenotype, non-suppressible by Pex2^UAS.cCa/Scer\GAL4^Hsp83.PA

(Chen et al., 2010)

Pex10¹⁰ has abnormal developmental rate phenotype, non-suppressible by Pex2^UAS.cCa/Scer\GAL4^Hsp83.PA

(Chen et al., 2010)

Pex10¹⁰ has abnormal cytokinesis phenotype, non-suppressible by Pex2^UAS.cCa/Scer\GAL4^Hsp83.PA

(Chen et al., 2010)

Pex10¹⁰ has abnormal meiotic cell cycle phenotype, non-suppressible by Pex2^UAS.cCa/Scer\GAL4^Hsp83.PA

(Chen et al., 2010)

Pex10¹⁰ has male sterile phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Pex2^UAS.cCa

(Chen et al., 2010)

Pex10¹⁰ has abnormal developmental rate phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Pex2^UAS.cCa

(Chen et al., 2010)

Pex10¹⁰ has abnormal cytokinesis phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Pex2^UAS.cCa

(Chen et al., 2010)

Pex10¹⁰ has abnormal meiotic cell cycle phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Pex2^UAS.cCa

(Chen et al., 2010)

Pex10¹⁰ has male sterile phenotype, non-suppressible by Scer\GAL4^nanos.PG/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has abnormal developmental rate phenotype, non-suppressible by Scer\GAL4^nanos.PG/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has abnormal cytokinesis phenotype, non-suppressible by Scer\GAL4^nanos.PG/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has abnormal meiotic cell cycle phenotype, non-suppressible by Scer\GAL4^nanos.PG/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has male sterile phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has abnormal developmental rate phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has abnormal cytokinesis phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has abnormal meiotic cell cycle phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has male sterile phenotype, non-suppressible by Scer\GAL4^Hsp83.PA/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has abnormal developmental rate phenotype, non-suppressible by Scer\GAL4^Hsp83.PA/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has abnormal cytokinesis phenotype, non-suppressible by Scer\GAL4^Hsp83.PA/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has abnormal meiotic cell cycle phenotype, non-suppressible by Scer\GAL4^Hsp83.PA/Pex2^UAS.mCherry

(Chen et al., 2010)

Suppressor of

Statement

Reference

pex10[+]/Pex10¹⁰ is a suppressor | partially of male sterile phenotype of bond^Z5274

(Chen et al., 2010)

pex10[+]/Pex10¹⁰ is a suppressor | partially of abnormal cytokinesis phenotype of bond^Z5274

(Chen et al., 2010)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Pex10¹⁰ has spermatid phenotype, non-suppressible by Scer\GAL4^nanos.PG/Pex2^UAS.cCa

(Chen et al., 2010)

Pex10¹⁰ has spermatid phenotype, non-suppressible by Pex2^UAS.cCa/Scer\GAL4^Hsp83.PA

(Chen et al., 2010)

Pex10¹⁰ has testis phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has testis phenotype, non-suppressible by Scer\GAL4^Hsp83.PA/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has spermatid phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Pex2^UAS.cCa

(Chen et al., 2010)

Pex10¹⁰ has spermatid phenotype, non-suppressible by Scer\GAL4^nanos.PG/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has spermatid phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has spermatid phenotype, non-suppressible by Scer\GAL4^Hsp83.PA/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex10¹⁰ has testis phenotype, non-suppressible by Scer\GAL4^nanos.PG/Pex2^UAS.cCa

(Chen et al., 2010)

Pex10¹⁰ has testis phenotype, non-suppressible by Pex2^UAS.cCa/Scer\GAL4^Hsp83.PA

(Chen et al., 2010)

Pex10¹⁰ has testis phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Pex2^UAS.cCa

(Chen et al., 2010)

Pex10¹⁰ has testis phenotype, non-suppressible by Scer\GAL4^nanos.PG/Pex2^UAS.mCherry

(Chen et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of pex2^Scer\UAS.cCa under the control of Scer\GAL4^nos.PG, Scer\GAL4^Hsp83.PA or Scer\GAL4^ptc-559.1 fails to suppress the male sterility and spermatocyte developmental phenotypes associated with pex10¹⁰ mutants.

Expression of pex2^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^nos.PG, Scer\GAL4^Hsp83.PA or Scer\GAL4^ptc-559.1 fails to suppress the male sterility and spermatocyte developmental phenotypes associated with pex10¹⁰ mutants.

(Chen et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Pex10#10

(Du et al., 2020)

Pex10^#10

(Du et al., 2020)

Pex10¹⁰

pex10¹⁰

(Chen et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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