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General Information
Symbol
Dmel\LamCΔN.UAS.cSa
Species
D. melanogaster
Name
FlyBase ID
FBal0245197
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ΔN
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of a modified form of LamC, lacking the first 42 amino acids.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Expression of LamCΔN.Scer\UAS.cSa under the control of Scer\GAL4C57 leads to larval locomotion defects and semi-lethality at the pupal stage. A subset of the larval body wall muscles show abnormally shaped and spaced nuclei, and disorganization of the actin cytoskeleton.
Body wall muscles of third instar larvae expressing LamCΔN.Scer\UAS.cSa under the control of Scer\GAL4C57 have misshapen nuclei. These muscle nuclei deform significantly more than control nuclei under applied strain.
Nuclei in the body wall muscles of third instar larvae expressing LamCΔN.Scer\UAS.cSa under the control of Scer\GAL4C57 are sometimes misshapen and elongated and appear to contain highly condensed chromatin. There is an absence of tyrosinated tubulin network around the misshapen nuclei. Approximately 21% of the body wall muscles in third instar larvae expressing LamCΔN.Scer\UAS.cSa under the control of Scer\GAL4C57 show disruptions in the striated pattern. Phalloidin staining fibers are seen in approximately 27% of the nuclei of the body wall muscles, suggesting the presence of actin filament formation in the nucleus. Adult escapers expressing LamCΔN.Scer\UAS.cSa under the control of Scer\GAL4C57 show defects in the third legs; the femur and tibia are shorter and thinner than normal and the basitarsus and last tarsal segments are twisted and shorter than normal. These defects are apparent during the pupal stage, where the legs are seen to curl around the wing in the mutant late stage pupae. At 6 hours after puparium formation the mutant leg discs are misshapen and stunted in elongation compared to wild type.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
LamCΔN.Scer\UAS.cSa
LamCΔN.UAS.cSa
Name Synonyms
Secondary FlyBase IDs
    References (4)