Expression of LamCΔN.Scer\UAS.cSa under the control of Scer\GAL4C57 leads to larval locomotion defects and semi-lethality at the pupal stage. A subset of the larval body wall muscles show abnormally shaped and spaced nuclei, and disorganization of the actin cytoskeleton.
Body wall muscles of third instar larvae expressing LamCΔN.Scer\UAS.cSa under the control of Scer\GAL4C57 have misshapen nuclei. These muscle nuclei deform significantly more than control nuclei under applied strain.
Nuclei in the body wall muscles of third instar larvae expressing LamCΔN.Scer\UAS.cSa under the control of Scer\GAL4C57 are sometimes misshapen and elongated and appear to contain highly condensed chromatin. There is an absence of tyrosinated tubulin network around the misshapen nuclei.
Approximately 21% of the body wall muscles in third instar larvae expressing LamCΔN.Scer\UAS.cSa under the control of Scer\GAL4C57 show disruptions in the striated pattern. Phalloidin staining fibers are seen in approximately 27% of the nuclei of the body wall muscles, suggesting the presence of actin filament formation in the nucleus.
Adult escapers expressing LamCΔN.Scer\UAS.cSa under the control of Scer\GAL4C57 show defects in the third legs; the femur and tibia are shorter and thinner than normal and the basitarsus and last tarsal segments are twisted and shorter than normal. These defects are apparent during the pupal stage, where the legs are seen to curl around the wing in the mutant late stage pupae. At 6 hours after puparium formation the mutant leg discs are misshapen and stunted in elongation compared to wild type.
