FlyBase Allele Report: Dmel\Mad[JF01264]

General Information

Symbol

Dmel\Mad^JF01264

Species

D. melanogaster

Name

FlyBase ID

FBal0245556

Feature type

allele

Associated gene

Dmel\Mad

Associated Insertion(s)

Carried in Construct

P{TRiP.JF01264}

Also Known As

UAS-Mad^RNAi, Mad RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2008)

Mutagen

in vitro construct

(Ni et al., 2008)

Progenitor genotype

Carried in construct

P{TRiP.JF01264}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Ni et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF01264

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023, Lodge et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^dsx.KI

(Redhai et al., 2016)

abnormal size | adult stage, with Scer\GAL4^esg.PU

(Redhai et al., 2016)

abnormal size | larval stage | somatic clone, with Scer\GAL4^Act5C.PP

(Bakopoulos et al., 2023)

Phenotype Manifest In

embryonic/larval fat body | larval stage, with Scer\GAL4^r4

(Bakopoulos et al., 2023)

lysosome | adult stage, with Scer\GAL4^dsx.KI

(Redhai et al., 2016)

male accessory gland secondary cell, with Scer\GAL4^dsx.KI

(Redhai et al., 2016)

male accessory gland secondary cell, with Scer\GAL4^esg.PU

(Redhai et al., 2016)

multivesicular body | adult stage, with Scer\GAL4^dsx.KI

(Redhai et al., 2016)

nucleus | adult stage, with Scer\GAL4^esg.PU

(Redhai et al., 2016)

secretory vesicle | adult stage, with Scer\GAL4^dsx.KI

(Redhai et al., 2016)

wing disc | somatic clone, with Scer\GAL4^Act5C.PP

(Bakopoulos et al., 2023)

Detailed Description

Statement

Reference

The adulthood-only expression of Mad^JF01264 under the control of Scer\GAL4^esg.PU (in combination with Gal80[ts], for temporal regulation of expression) leads to a significant decrease in the relative and absolute size of secondary cell nuclei compared to neighboring main cells and wild-type controls; this expression does not affect the size of main cell nuclei compared to controls. The adulthood-only expression under the control of Scer\GAL4^dsx.KI (and Gal80[ts]) leads to a significant decrease in the number of secretory vesicles within accessory gland secondary cells, associated with a significant decrease in the size of the largest multivesicular body/lysosome, as compared to controls; there is also a severe decrease in the secretion of (CD63-positive) exosomes into the accessory gland lumen.

(Redhai et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Scer\GAL4^Mhc.PK/Mad^JF01264 is a non-suppressor of atrophy | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Scer\GAL4^r4/Mad^JF01264 is a non-suppressor of atrophy | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Mad^JF01264, Scer\GAL4^r4 has embryonic/larval fat body | larval stage phenotype, non-enhanceable by Pi3K21B^UAS.Tag:HA, Scer\GAL4^r4

(Bakopoulos et al., 2023)

NOT suppressed by

Statement

Reference

Mad^JF01264, Scer\GAL4^r4 has embryonic/larval fat body | larval stage phenotype, non-suppressible by Pi3K21B^UAS.Tag:HA, Scer\GAL4^r4

(Bakopoulos et al., 2023)

Suppressor of

Statement

Reference

Scer\GAL4^r4/Mad^JF01264 is a suppressor of extracellular matrix | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

InR^A1325D.UAS, Scer\GAL4^r4, Mad^JF01264 is a suppressor | partially of muscle attachment site | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Scer\GAL4^r4/Mad^JF01264 is a suppressor | partially of muscle attachment site | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Scer\GAL4^r4/Mad^JF01264 is a suppressor | partially of larval muscle system | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

InR^A1325D.UAS, Scer\GAL4^r4, Mad^JF01264 is a suppressor | partially of larval muscle system | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Scer\GAL4^r4/Mad^JF01264 is a suppressor of embryonic/larval fat body | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Mhc.PK/Mad^JF01264 is a non-suppressor of A1-7 ventral longitudinal muscle 3 | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Scer\GAL4^r4/Mad^JF01264 is a non-suppressor of A1-7 ventral longitudinal muscle 3 | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Scer\GAL4^Mhc.PK/Mad^JF01264 is a non-suppressor of muscle attachment site | larval stage phenotype of Ncra\QF^QF2.Act5C.PL, Ras85D^V12.QUAS.cLa, scrib^{QUAS.HMS01490}

(Bakopoulos et al., 2023)

Mad^JF01264, Scer\GAL4^tin.CΔ4 is a non-suppressor of adult heart phenotype of Scer\GAL4^tin.CΔ4, yki^{S168A.UAS.Tag:V5}

(Yu et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Mad^JF01264 does not suppress the cardiac hypertrophy seen when yki^{S168A.Scer\UAS.T:SV5\V5} is expressed under the control of Scer\GAL4^tin.CΔ4.

(Yu et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

31316

y¹ v¹; P{TRiP.JF01264}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Mad^JF01264

mad^Ri

(Lodge et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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