FB2025_01 , released February 20, 2025
Allele: Dmel\mahj1
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General Information
Symbol
Dmel\mahj1
Species
D. melanogaster
Name
FlyBase ID
FBal0246066
Feature type
allele
Associated gene
Dmel\mahj
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Tamori et al., 2010)
Progenitor genotype
P{GT1}asRNA:CR42547GT-000304
(Tamori et al., 2010)
Cytology
Description

Imprecise excision of the progenitor insertion, resulting in a 1633bp deletion that includes part of exon 9, exons 10 and 11 and the 3' UTR of mahj.

(Tamori et al., 2010)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2R:21,630,411..21,632,043 [+]
Comment:

Estimated boundaries of a 1633 bp deletion resulting from the imprecise excision of P{GT1}asRNA:CR42547GT-000304, which removes part of exon 9, exons 10 and 11 and the 3' UTR of mahj.

(Tamori et al., 2010)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      mahj1 homozygotes and mahj1/Df(2R)XE-2900 transheterozygotes exhibit signs of quiescence in the larval brain: neuroblasts show a significant decrease in cell divisions (DNA replication/EdU staining); are significantly decreased in number; and frequently show a primary cellular process, whereas control neuroblasts show a typically rounded morphology. In agreement, mahj1 homozygous larvae exhibit a small brain, as compared to controls.

      Like in controls, mushroom body neuroblast lineages in mahj1 homozygous larvae are able to under cell division (DNA replication/EdU staining).

      (Ly et al., 2019)

      Homozygous and mahj1/Df(2R)XE-2900 animals develop more slowly than normal.

      Homozygous larvae do not have any detectable morphological defects.

      Analysis of homozygous clones in the wing disc shows that 72 hours after clone induction, both homozygous and wild-type sibling clones are readily detected. However, at 96 hours after clone induction, the homozygous clones are smaller than the sibling clones, and by 120 hours after clone induction, most of the homozygous clones have disappeared from the wing disc epithelium. Homozygous cells adjacent to wild type or heterozygous cells frequently undergo apoptosis, whereas homozygous cells that are not adjacent to wild type or heterozygous cells rarely undergo apoptosis. The majority of apoptotic cells are basally extruded from the disc epithelium, but some remain within the epithelial layer. Basally extruded homozygous cells which are not apoptotic are also sometimes seen.

      (Tamori et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      NOT suppressed by
      Phenotype Manifest In
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      mahj1 has wing disc | somatic clone phenotype, non-suppressible | somatic clone by Scer\GAL4Tub.PU/l(2)glUAS.Tag:MYC

      (Tamori et al., 2010)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of pucScer\UAS.cMa under the control of Scer\GAL4tub.PU strongly suppresses the apoptosis that is seen in mahj1 clones in the wing disc. Basal extrusion of mutant cells is not fully blocked in these discs.

      Expression of l(2)glScer\UAS.T:Hsap\MYC under the control of Scer\GAL4tub.PU does not affect the phenotype of mahj1 clones in the wing disc; the cells undergo apoptosis and are basally extruded.

      (Tamori et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub.PU strongly suppresses the apoptosis that is seen in mahj1 clones in the wing disc. Basal extrusion of mutant cells is not fully blocked in these discs.

      (Tamori et al., 2010)
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      mahj1
      (Ly et al., 2019, Nagata et al., 2019, Tamori et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)