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General Information
Symbol
Dmel\mahj1
Species
D. melanogaster
Name
FlyBase ID
FBal0246066
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Comment:

Estimated boundaries of a 1633 bp deletion resulting from the imprecise excision of P{GT1}CR42547GT-000304, which removes part of exon 9, exons 10 and 11 and the 3' UTR of mahj.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion, resulting in a 1633bp deletion that includes part of exon 9, exons 10 and 11 and the 3' UTR of mahj.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

mahj1 homozygotes and mahj1/Df(2R)XE-2900 transheterozygotes exhibit signs of quiescence in the larval brain: neuroblasts show a significant decrease in cell divisions (DNA replication/EdU staining); are significantly decreased in number; and frequently show a primary cellular process, whereas control neuroblasts show a typically rounded morphology. In agreement, mahj1 homozygous larvae exhibit a small brain, as compared to controls.

Like in controls, mushroom body neuroblast lineages in mahj1 homozygous larvae are able to under cell division (DNA replication/EdU staining).

Homozygous and mahj1/Df(2R)XE-2900 animals develop more slowly than normal.

Homozygous larvae do not have any detectable morphological defects.

Analysis of homozygous clones in the wing disc shows that 72 hours after clone induction, both homozygous and wild-type sibling clones are readily detected. However, at 96 hours after clone induction, the homozygous clones are smaller than the sibling clones, and by 120 hours after clone induction, most of the homozygous clones have disappeared from the wing disc epithelium. Homozygous cells adjacent to wild type or heterozygous cells frequently undergo apoptosis, whereas homozygous cells that are not adjacent to wild type or heterozygous cells rarely undergo apoptosis. The majority of apoptotic cells are basally extruded from the disc epithelium, but some remain within the epithelial layer. Basally extruded homozygous cells which are not apoptotic are also sometimes seen.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Phenotype Manifest In
Suppressed by
NOT suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Expression of pucScer\UAS.cMa under the control of Scer\GAL4tub.PU strongly suppresses the apoptosis that is seen in mahj1 clones in the wing disc. Basal extrusion of mutant cells is not fully blocked in these discs.

Expression of l(2)glScer\UAS.T:Hsap\MYC under the control of Scer\GAL4tub.PU does not affect the phenotype of mahj1 clones in the wing disc; the cells undergo apoptosis and are basally extruded.

Xenogenetic Interactions
Statement
Reference

Expression of BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub.PU strongly suppresses the apoptosis that is seen in mahj1 clones in the wing disc. Basal extrusion of mutant cells is not fully blocked in these discs.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (3)