FlyBase Allele Report: Dmel\Stat92E[ΔNΔC.UASp.Tag:HA]

General Information

Symbol

Dmel\Stat92E^{ΔNΔC.UASp.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0246109

Feature type

allele

Associated gene

Dmel\Stat92E

Associated Insertion(s)

Carried in Construct

P{UASp-Stat92E.ΔNΔC}

Also Known As

UAS-3HA-stat92E^ΔNΔC, UAS-stat92e^ΔNΔC, UAS-Stat92EΔNΔC

Key Links

Transgenic product class

inframe_deletion

(Ekas et al., 2010)

missense_variant

(Ekas et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Ekas et al., 2010)

missense_variant

(Ekas et al., 2010)

Mutagen

in vitro construct

(Ekas et al., 2010)

Progenitor genotype

Stat92E^UASp.Tag:HA

(Ekas et al., 2010)

Carried in construct

P{UASp-Stat92E.ΔNΔC}

Cytology

Description

Deletion of sequences encoding amino acid residues 1-333 and 725-761.

(Ekas et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Stat92E

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with scrib^KK101128, RhoGEF2^HMS01118

(Tamori et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by l(2)gl^GD4047

(Tamori et al., 2016)

modeled by scrib^KK101128

(Tamori et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

melanotic mass phenotype | somatic clone, with Scer\GAL4^Act5C.PI

(Ekas et al., 2010)

visible, with Scer\GAL4^ey.PH

(Ekas et al., 2010)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Ekas et al., 2010)

larval optic anlage, with Scer\GAL4^c768

(Zhou and Luo, 2013)

melanotic mass | somatic clone, with Scer\GAL4^Act5C.PI

(Ekas et al., 2010)

Detailed Description

Statement

Reference

The clonal expression of Stat92E^{ΔNΔC.UASp.Tag:HA} under the control of Scer\GAL4^Act5C.PI does not lead to any obvious third instar larval wing discs, as compared to controls.

(Tamori et al., 2016)

Expression of Stat92E^{ΔNΔC.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^c768 results in overgrowth of the neuroepithelium in the third larval instar optic lobe.

(Zhou and Luo, 2013)

Expression of Stat92E^{ΔNΔC.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^ey.PH results in moderate eye overgrowth.

Expression of Stat92E^{ΔNΔC.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^Act5C.PI in otherwise wild-type clones results in melanotic tumours in third instar larvae. Expression of Stat92E^{ΔNΔC.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^Act5C.PI in Stat92E^85C9 clones does not result in melanotic tumours.

(Ekas et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^Act5C.PI/Stat92E^{ΔNΔC.UASp.Tag:HA} is an enhancer of neoplasia | third instar larval stage | somatic clone phenotype of Scer\GAL4^Act5C.PI, l(2)gl^GD4047

(Tamori et al., 2016)

Scer\GAL4^Act5C.PI/Stat92E^{ΔNΔC.UASp.Tag:HA} is an enhancer of neoplasia | third instar larval stage | somatic clone phenotype of Scer\GAL4^Act5C.PI, scrib^KK101128

(Tamori et al., 2016)

Other

Statement

Reference

RhoGEF2^HMS01118, Scer\GAL4^Act5C.PI, Stat92E^{ΔNΔC.UASp.Tag:HA}, scrib^KK101128 has neoplasia | somatic clone | third instar larval stage phenotype

(Tamori et al., 2016)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^Act5C.PI/Stat92E^{ΔNΔC.UASp.Tag:HA} is an enhancer of wing disc | third instar larval stage | somatic clone phenotype of Scer\GAL4^Act5C.PI, l(2)gl^GD4047

(Tamori et al., 2016)

Scer\GAL4^Act5C.PI/Stat92E^{ΔNΔC.UASp.Tag:HA} is an enhancer of wing hinge primordium | third instar larval stage | somatic clone phenotype of Scer\GAL4^Act5C.PI, l(2)gl^GD4047

(Tamori et al., 2016)

Scer\GAL4^Act5C.PI/Stat92E^{ΔNΔC.UASp.Tag:HA} is an enhancer of wing disc | third instar larval stage | somatic clone phenotype of Scer\GAL4^Act5C.PI, scrib^KK101128

(Tamori et al., 2016)

Scer\GAL4^Act5C.PI/Stat92E^{ΔNΔC.UASp.Tag:HA} is an enhancer of wing hinge primordium | third instar larval stage | somatic clone phenotype of Scer\GAL4^Act5C.PI, scrib^KK101128

(Tamori et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^Hml.Δ/Stat92E^{ΔNΔC.UASp.Tag:HA} is a suppressor of embryonic/larval lymph gland phenotype of Scer\GAL4^Hml.Δ, bip1^NIG.7574R

(Mondal et al., 2014)

NOT Suppressor of

Statement

Reference

Stat92E^{ΔNΔC.UASp.Tag:HA}, Scer\GAL4^c768 is a non-suppressor of larval optic anlage phenotype of RPA3^GD4799, Scer\GAL4^c768

(Zhou and Luo, 2013)

Other

Statement

Reference

RhoGEF2^HMS01118, Scer\GAL4^Act5C.PI, Stat92E^{ΔNΔC.UASp.Tag:HA}, scrib^KK101128 has wing pouch | somatic clone | third instar larval stage phenotype

(Tamori et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Stat92E^{ΔNΔC.UASp.Tag:HA} enhances the wing disc hinge overgrowths induced by the clonal expression of either l(2)gl^GD4047 or scrib^KK101128 under the control of Scer\GAL4^Act5C.PI (together with Dicer-2, for efficient RNAi), leading to large tumor masses; this co-expression still does not lead to overgrowths in the wing pouch area and does not affect the basal delamination/extrusion observed upon scrib^KK101128 single expression.

The clonal co-expression of scrib^KK101128, RhoGEF2^HMS01118 and Stat92E^{ΔNΔC.UASp.Tag:HA} under the control of Scer\GAL4^Act5C.PI (together with Dicer-2, for efficient RNAi) in third instar larval wing discs leads to overgrowths in the wing pouch domain, which does not occur upon scrib^KK101128 single expression or scrib^KK101128, RhoGEF2^HMS01118 double expression.

(Tamori et al., 2016)

Co-expression of Stat92E^{ΔNΔC.Scer\UAS.P\T.T:Ivir\HA1} does not rescue the loss of neuroepithelium cells seen in the optic lobe of third instar larvae expressing RPA3^GD4799 under the control of Scer\GAL4^c768.

(Zhou and Luo, 2013)

The small eye phenotype but not the outstretched wing phenotype of Df(1)os¹/Y animals is rescued by expression of Stat92E^{ΔNΔC.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^ey.PH.

(Ekas et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Stat92E^{ΔNΔC.UASp.Tag:HA}/Scer\GAL4^ey.PH rescues Stat92E^85C9

(Ekas et al., 2010)

Partially rescues

Scer\GAL4^Tub.PU/Stat92E^{ΔNΔC.UASp.Tag:HA} partially rescues Stat92E^85C9

(Ekas et al., 2010)

Comments

The mutant eye phenotype seen in mosaic animals in which the eye-antennal disc is almost completely homozygous for Stat92E^85C9 is completely rescued by Stat92E^{ΔNΔC.Scer\UAS.P\T.T:Ivir\HA1} expressed under the control of Scer\GAL4^ey.PH.

Expression of Stat92E^{ΔNΔC.Scer\UAS.P\T.T:Ivir\HA1} under the control of Scer\GAL4^tub.PU significantly delays the lethal phase of Stat92E^85C9 homozygotes, such that most of the animals die as third instar larvae or early pupae.

(Ekas et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

STAT^CA

(Tamori et al., 2016)

Stat92E^{ΔNΔC.Scer\UAS.P\T.T:Ivir\HA1}

Stat92E^{ΔNΔC.UASp.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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