FlyBase Allele Report: Dmel\Eph[UAS.Tag:MYC]

General Information

Symbol

Dmel\Eph^UAS.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0246162

Feature type

allele

Associated gene

Dmel\Eph

Associated Insertion(s)

Carried in Construct

P{UAS-Eph.myc}

Also Known As

UAS-eph-myc

Key Links

Transgenic product class

cDNA

(Scully et al., 1999)

wild_type

(Scully et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Scully et al., 1999)

wild_type

(Scully et al., 1999)

Mutagen

in vitro construct

(Scully et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-Eph.myc}

Cytology

Description

UAS regulatory sequences drive expression of the Eph coding region which is tagged at the C-terminal end with five copies of Tag:MYC. The 5' and 3' untranslated regions are not included in the construct, and nucleotides 475-486 of the cDNA sequence have been replaced with a Cavener consensus start site placed directly in front of the putative signal sequence (TTTCGAGAATGT has been changed to CAATCAAAATGG).

(Scully et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Eph

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates tauopathy

modeled by Hsap\MAPT^4xA.UAS.2N4R

(Dourlen et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm, with Scer\GAL4^tim.PE

(Buhl et al., 2022)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ey-OK107

(Buhl et al., 2022)

abnormal neurophysiology | adult stage, with Scer\GAL4^tim.PE

(Buhl et al., 2022)

abnormal sleep, with Scer\GAL4^tim.PE

(Buhl et al., 2022)

viable, with Scer\GAL4^A80

(Scully et al., 1999)

viable, with Scer\GAL4^elav.PLu

(Scully et al., 1999)

viable, with Scer\GAL4^elav.PLu, Scer\GAL4^sca-537.4

(Scully et al., 1999)

viable, with Scer\GAL4^elav-C155

(Scully et al., 1999)

viable, with Scer\GAL4^ftz.ng

(Scully et al., 1999)

viable, with Scer\GAL4^GMR.PF

(Scully et al., 1999)

viable, with Scer\GAL4^sca-537.4

(Scully et al., 1999)

Phenotype Manifest In

adult mushroom body alpha'-lobe, with Scer\GAL4^ey-OK107

(Buhl et al., 2022)

adult mushroom body alpha-lobe, with Scer\GAL4^ey-OK107

(Buhl et al., 2022)

l-LNv neuron, with Scer\GAL4^tim.PE

(Buhl et al., 2022)

Detailed Description

Statement

Reference

Expression of Eph^{Scer\UAS.T:Hsap\MYC} under the control of any one of Scer\GAL4^elav-C155, Scer\GAL4^sca-537.4, Scer\GAL4^elav.PLu, Scer\GAL4^ftz.ng, Scer\GAL4^A80 or Scer\GAL4^GMR.PF does not result in a mutant phenotype.

Expression of Eph^{Scer\UAS.T:Hsap\MYC} under the simultaneous control of Scer\GAL4^sca-537.4 and Scer\GAL4^elav.PLu does not result in a mutant phenotype.

(Scully et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^GH146/Eph^UAS.Tag:MYC is an enhancer of abnormal neuroanatomy | adult stage | somatic clone phenotype of meigo¹

(Sekine et al., 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^GH146/Eph^UAS.Tag:MYC is an enhancer of adult antennal lobe projection neuron | somatic clone phenotype of meigo¹

(Sekine et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Eph^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GH146 enhances the targeting defects seen in homozygous meigo¹ DL1 projection neuron clones.

(Sekine et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

59844

w^*; P{UAS-Eph.myc}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Eph^{Scer\UAS.T:Hsap\MYC}

Eph^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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