FlyBase Allele Report: Dmel\WASp[3D3-035]

General Information

Symbol

Dmel\WASp^3D3-035

Species

D. melanogaster

Name

FlyBase ID

FBal0246285

Feature type

allele

Associated gene

Dmel\WASp

Associated Insertion(s)

Carried in Construct

Genomic Maps

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Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Schafer et al., 2007)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point mutation

3R:28,825,619..28,825,619 [-]

Nucleotide change:

T28825619A

Amino acid change:

L472term | WASp-PA; L472term | WASp-PB; L439term | WASp-PC; L472term | WASp-PD

Reported amino acid change:

?472term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Schafer et al., 2007)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

WASp^3D3-035 alleles carry a point mutation that leads to loss of the CA domain of the VCA module, essential for binding of the Arp2/3 complex.

(Berger et al., 2008)

Amino acid replacement: ?472term.

(Schafer et al., 2007)

The premature stop codon is predicted to result in a protein that lacks the CA domain and part of one of the V domains, removing the Arp2/3 complex binding domain and resulting in a dominant negative effect.

(Schafer et al., 2007)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

lethal - all die before end of embryonic stage

(Schafer et al., 2007)

some die during embryonic stage

(Schafer et al., 2007)

Phenotype Manifest In

dorsal acute muscle (with Df(3R)3450)

(Schafer et al., 2007)

dorsal acute muscle | embryonic stage

(Berger et al., 2008)

embryonic myoblast

(Schafer et al., 2007)

embryonic myoblast (with Df(3R)3450)

(Schafer et al., 2007)

embryonic myoblast (with WASp¹)

(Schafer et al., 2007)

embryonic myoblast (with WASp³)

(Schafer et al., 2007)

muscle attachment site

(Schafer et al., 2007)

muscle attachment site (with Df(3R)3450)

(Schafer et al., 2007)

myoblast | embryonic stage

(Berger et al., 2008)

plasma membrane | embryonic stage

(Berger et al., 2008)

Detailed Description

Statement

Reference

WASp^3D3-035 mutants exhibit normal visceral mesoderm development, resulting in normal gut development.

(Eriksson et al., 2010)

Ultrathin sections of WASp^3D3-035 embryos reveal that the membrane breakdown step in myoblast fusion fails to complete.

(Berger et al., 2008)

WASp^3D3-035 mutants display a striking myoblast fusion defect. Small mini muscles are surrounded by myoblasts that fail to fuse and often do not attach correctly,

WASp^3D3-035/Df(3R)3450 mutants exhibit myoblast fusion as well as attachment defects.

The majority of founder cells are correctly specified in WASp^3D3-035/Df(3R)3450 transheterozygous mutants.

An average of 3 to 4 nuclei are found in DA1 mini muscles in WASp^3D3-035/Df(3R)3450 mutant embryos compared to up to 14 nuclei in wild-type. WASp^3D3-035/Df(3R)3450 transheterozygous mutants appear to often stop fusion after precursor cell formation.

WASp^3D3-035/WASp³ and WASp^3D3-035/WASp¹ transheterozygotes exhibit a myoblast fusion phenotype.

(Schafer et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

WASp^3D3-035 has lethal | embryonic stage phenotype, non-enhanceable by Hem[+]/Hem^J4-48

(Schafer et al., 2007)

NOT suppressed by

Statement

Reference

WASp^3D3-035 has lethal | embryonic stage phenotype, non-suppressible by Hem[+]/Hem^J4-48

(Schafer et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

WASp^3D3-035 has dorsal acute muscle | embryonic stage phenotype, enhanceable by Arp3^schwachling

(Berger et al., 2008)

WASp^3D3-035 has myoblast | embryonic stage phenotype, enhanceable by Arp3^schwachling

(Berger et al., 2008)

WASp^3D3-035 has dorsal acute muscle | embryonic stage phenotype, enhanceable by Arp3^EP3640

(Berger et al., 2008)

WASp^3D3-035 has myoblast | embryonic stage phenotype, enhanceable by Arp3^EP3640

(Berger et al., 2008)

NOT Enhanced by

Statement

Reference

WASp^3D3-035 has embryonic myoblast phenotype, non-enhanceable by Hem[+]/Hem^J4-48

(Schafer et al., 2007)

WASp^3D3-035 has muscle attachment site phenotype, non-enhanceable by Hem[+]/Hem^J4-48

(Schafer et al., 2007)

NOT suppressed by

Statement

Reference

WASp^3D3-035 has embryonic myoblast phenotype, non-suppressible by Hem[+]/Hem^J4-48

(Schafer et al., 2007)

WASp^3D3-035 has muscle attachment site phenotype, non-suppressible by Hem[+]/Hem^J4-48

(Schafer et al., 2007)

Enhancer of

Statement

Reference

WASp^3D3-035 is an enhancer of dorsal acute muscle | embryonic stage phenotype of Arp3^EP3640

(Berger et al., 2008)

WASp^3D3-035 is an enhancer of myoblast | embryonic stage phenotype of Arp3^EP3640

(Berger et al., 2008)

WASp^3D3-035 is an enhancer of dorsal acute muscle | embryonic stage phenotype of Arp3^schwachling

(Berger et al., 2008)

WASp^3D3-035 is an enhancer of myoblast | embryonic stage phenotype of Arp3^schwachling

(Berger et al., 2008)

NOT Enhancer of

Statement

Reference

WASp^3D3-035 is a non-enhancer of embryonic myoblast phenotype of Hem^J4-48

(Schafer et al., 2007)

Suppressor of

Statement

Reference

WASp^3D3-035/WASp[+] is a suppressor | partially of embryonic myoblast phenotype of Hem^J4-48

(Schafer et al., 2007)

Other

Statement

Reference

Arp3^schwachling, WASp^3D3-035 has embryonic myoblast | embryonic stage 16 phenotype

(Rudolf et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Aberrant migration of the longitudinal visceral muscle founder cells is observed in stage 13 WASp^3D3-035;Arp3^schwachling double homozygote embryos and many unfused somatic myoblasts are seen at stage 16 but both the longitudinal visceral muscles and gut morphology appear normal.

(Rudolf et al., 2014)

Arp66B^schwachling WASp^3D3-035 double mutants exhibit normal visceral mesoderm development, resulting in normal gut development.

(Eriksson et al., 2010)

The DA1 muscle in Arp66B^schwachling WASp^3D3-035 double mutants is mainly mononucleated, indicating that myoblasts fail to fuse completely.

The myoblast-fusion phenotype found in WASp^3D3-035 or Arp66B^schwachling single mutant embryos is strongly enhanced in the double mutant. Analysis of the DA1 muscle in the double mutant reveals only one nucleus per hemisegment, indicating that fusion is completely blocked in the double mutant.

Myoblast fusion does not occur in DA1 muscles in Arp66B^EP3640, WASp^3D3-035 double mutants.

(Berger et al., 2008)

Hem^J4-48/WASp^3D3-035 double mutants exhibit a similar myoblast fusion phenotype as Hem^J4-48 single mutants. A Hem^J4-48 heterozygous background does not influence the WASp^3D3-035 homozygous mutant phenotype. A WASp^3D3-035 heterozygous background partially suppresses the block of fusion observed in Hem^J4-48 homozygous mutants.

(Schafer et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

WASp³

(Schafer et al., 2007)

WASp¹

(Schafer et al., 2007)

Rescued by

Df(3R)3450/WASp^3D3-035 is rescued by Scer\GAL4^twi.PG/WASp^UAS.cBa

(Schafer et al., 2007)

Not rescued by

Df(3R)3450/WASp^3D3-035 is not rescued by Scer\GAL4^{kirre-rP298-G4}/WASp^UAS.cBa

(Schafer et al., 2007)

Comments

Expression of WASp^Scer\UAS.cBa in WASp^3D3-035/Df(3R)3450 mutants under the control of Scer\GAL4^twi.PG restores almost the complete muscle pattern.

Expression of WASp^Scer\UAS.cBa specifically in founder cells (under the control of Scer\GAL4^kirre-rP298) in WASp^3D3-035/Df(3R)3450 mutants does not restore a wild-type muscle pattern. Instead muscles look thinner compared to wild-type and some muscles are even missing.

(Schafer et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

WASp^3D3-035

fm^3D3-035

(Schafer et al., 2007)

wasp^3D3-035

(Hamp et al., 2016, Rudolf et al., 2014, Eriksson et al., 2010, Berger et al., 2008, Schafer et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (5)

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