T28825619A
L472term | WASp-PA; L472term | WASp-PB; L439term | WASp-PC; L472term | WASp-PD
?472term
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
WASp3D3-035 alleles carry a point mutation that leads to loss of the CA domain of the VCA module, essential for binding of the Arp2/3 complex.
Amino acid replacement: ?472term.
The premature stop codon is predicted to result in a protein that lacks the CA domain and part of one of the V domains, removing the Arp2/3 complex binding domain and resulting in a dominant negative effect.
WASp3D3-035 mutants exhibit normal visceral mesoderm development, resulting in normal gut development.
Ultrathin sections of WASp3D3-035 embryos reveal that the membrane breakdown step in myoblast fusion fails to complete.
WASp3D3-035 mutants display a striking myoblast fusion defect. Small mini muscles are surrounded by myoblasts that fail to fuse and often do not attach correctly,
WASp3D3-035/Df(3R)3450 mutants exhibit myoblast fusion as well as attachment defects.
The majority of founder cells are correctly specified in WASp3D3-035/Df(3R)3450 transheterozygous mutants.
An average of 3 to 4 nuclei are found in DA1 mini muscles in WASp3D3-035/Df(3R)3450 mutant embryos compared to up to 14 nuclei in wild-type. WASp3D3-035/Df(3R)3450 transheterozygous mutants appear to often stop fusion after precursor cell formation.
WASp3D3-035/WASp3 and WASp3D3-035/WASp1 transheterozygotes exhibit a myoblast fusion phenotype.
WASp3D3-035 has lethal | embryonic stage phenotype, non-enhanceable by Hem[+]/HemJ4-48
WASp3D3-035 has lethal | embryonic stage phenotype, non-suppressible by Hem[+]/HemJ4-48
WASp3D3-035 has dorsal acute muscle cell | embryonic stage phenotype, enhanceable by Arp3schwachling
WASp3D3-035 has myoblast | embryonic stage phenotype, enhanceable by Arp3schwachling
WASp3D3-035 has dorsal acute muscle cell | embryonic stage phenotype, enhanceable by Arp3EP3640
WASp3D3-035 has myoblast | embryonic stage phenotype, enhanceable by Arp3EP3640
WASp3D3-035 has embryonic myoblast phenotype, non-enhanceable by Hem[+]/HemJ4-48
WASp3D3-035 has muscle attachment site phenotype, non-enhanceable by Hem[+]/HemJ4-48
WASp3D3-035 has embryonic myoblast phenotype, non-suppressible by Hem[+]/HemJ4-48
WASp3D3-035 has muscle attachment site phenotype, non-suppressible by Hem[+]/HemJ4-48
WASp3D3-035 is an enhancer of dorsal acute muscle cell | embryonic stage phenotype of Arp3EP3640
WASp3D3-035 is an enhancer of myoblast | embryonic stage phenotype of Arp3EP3640
WASp3D3-035 is an enhancer of dorsal acute muscle cell | embryonic stage phenotype of Arp3schwachling
WASp3D3-035 is an enhancer of myoblast | embryonic stage phenotype of Arp3schwachling
WASp3D3-035 is a non-enhancer of embryonic myoblast phenotype of HemJ4-48
WASp3D3-035/WASp[+] is a suppressor | partially of embryonic myoblast phenotype of HemJ4-48
Arp3schwachling, WASp3D3-035 has embryonic myoblast | embryonic stage 16 phenotype
Aberrant migration of the longitudinal visceral muscle founder cells is observed in stage 13 WASp3D3-035;Arp3schwachling double homozygote embryos and many unfused somatic myoblasts are seen at stage 16 but both the longitudinal visceral muscles and gut morphology appear normal.
Arp66Bschwachling WASp3D3-035 double mutants exhibit normal visceral mesoderm development, resulting in normal gut development.
The DA1 muscle in Arp66Bschwachling WASp3D3-035 double mutants is mainly mononucleated, indicating that myoblasts fail to fuse completely.
The myoblast-fusion phenotype found in WASp3D3-035 or Arp66Bschwachling single mutant embryos is strongly enhanced in the double mutant. Analysis of the DA1 muscle in the double mutant reveals only one nucleus per hemisegment, indicating that fusion is completely blocked in the double mutant.
Myoblast fusion does not occur in DA1 muscles in Arp66BEP3640, WASp3D3-035 double mutants.
HemJ4-48/WASp3D3-035 double mutants exhibit a similar myoblast fusion phenotype as HemJ4-48 single mutants. A HemJ4-48 heterozygous background does not influence the WASp3D3-035 homozygous mutant phenotype. A WASp3D3-035 heterozygous background partially suppresses the block of fusion observed in HemJ4-48 homozygous mutants.
Df(3R)3450/WASp3D3-035 is rescued by Scer\GAL4twi.PG/WASpUAS.cBa
Df(3R)3450/WASp3D3-035 is not rescued by Scer\GAL4kirre-rP298-G4/WASpUAS.cBa
Expression of WASpScer\UAS.cBa in WASp3D3-035/Df(3R)3450 mutants under the control of Scer\GAL4twi.PG restores almost the complete muscle pattern.
Expression of WASpScer\UAS.cBa specifically in founder cells (under the control of Scer\GAL4kirre-rP298) in WASp3D3-035/Df(3R)3450 mutants does not restore a wild-type muscle pattern. Instead muscles look thinner compared to wild-type and some muscles are even missing.