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General Information
Symbol
Dmel\Diap1Apoliner.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0246319
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Apoliner
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of 'Apoliner', a fluorescent sensor for caspase activity. The sensor is comprised of two fluorescent proteins (mRFP1 and EGFP) separated by sequence derived from the Diap1 gene. The Diap1 sequence corresponds to amino acids 1-146 and includes a caspase cleavage site, DQVDGV (this site has been mutated from the original DQVDNN to prevent degradation of the cleaved product by the N-end rule pathway). In addition, a transmembrane domain (Tag:M(mCd8a)) is fused to the N-terminal end of the mRFP1 sequence and a nuclear localization signal (Tag:NLS(SV40-largeT)) is fused to the N-terminal end of the EGFP sequence. In the absence of caspase activity, the transmembrane domain tethers both fluorescent proteins to the membrane. Upon cleavage of the DQVDGV site by active caspase, the EGFP protein is released, and can be targeted to the nucleus via the Tag:NLS(SV40-largeT) signal, while the mRFP1 protein remains tethered at the membrane.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
Comments
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Diap1Apoliner.Scer\UAS
Diap1Apoliner.UAS
thApoliner.Scer\UAS
thN21G.N22V.Scer\UAS.T:Mmus\Cd8a.T:Disc\RFP-mRFP.T:SV40\nls2.T:Avic\GFP-EGFP
Name Synonyms
Secondary FlyBase IDs
    References (11)