Allele Dmel\th^{N21G.N22V.Scer\UAS.T:Mmus\Cd8a.T:Disc\RFP-mRFP.T:SV40\nls2.T:Avic\GFP-EGFP}

General Information
Symbol	Dmel\th^{N21G.N22V.Scer\UAS.T:Mmus\Cd8a.T:Disc\RFP-mRFP.T:SV40\nls2.T:Avic\GFP-EGFP}	Species	D. melanogaster
Name		FlyBase ID	FBal0246319
Feature type	allele	Associated gene	Dmel\th

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - coding region fusion, in vitro construct - site directed mutagenesis
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Bardet et al., 2008) in vitro construct - regulatory fusion (Bardet et al., 2008) in vitro construct - site directed mutagenesis (Bardet et al., 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a fusion protein comprised of (from the amino- to the carboxy-terminus) a T:Mmus\Cd8a transmembrane domain, T:Disc\RFP[mRFP], a caspase sensitive site from th, a T:SV40\nls2 nuclear targeting signal and T:Avic\GFP[EGFP]. The actual cleavage site in the th fragment used (amino acids 1-146) is mutated (N21 to G and N22 to V) to prevent degradation of the cleaved product by the N-end rule pathway. (Bardet et al., 2008)
Carried in construct	P{UAS-Apoliner} (Berzsenyi et al., 2011, Zhai et al., 2012, Bardet et al., 2008, Suzanne et al., 2010)
Tagged with	T:Avic\GFP^EGFP (Bardet et al., 2008) T:Disc\RFP^mRFP (Bardet et al., 2008) T:Mmus\Cd8a (Bardet et al., 2008) T:SV40\nls2 (Bardet et al., 2008)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 3 )
Bloomington	32122 w^; P{UAS-Apoliner}5 32121 y¹ w^; P{UAS-Apoliner}9 32123 w^*; P{UAS-Apoliner}8/TM3, Sb¹
Notes on Origin
Discoverer

Comments
	th[N21G.N22V.Scer\UAS.T:Mmus\Cd8a.T:Disc\RFP-mRFP.T:SV40\nls2.T:Avic\GFP-EGFP] can be used as a reporter of caspase activity. (Bardet et al., 2008)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	th^{N21G.N22V.Scer\UAS.T:Mmus\Cd8a.T:Disc\RFP-mRFP.T:SV40\nls2.T:Avic\GFP-EGFP}
Name Synonym
Secondary FlyBase IDs

References ( 4 )
Research paper	Zhai et al., 2012, PLoS Genet. 8(3): e1002582 Antagonistic regulation of apoptosis and differentiation by the cut transcription factor represents a tumor-suppressing mechanism in Drosophila. [FBrf0217859] Berzsenyi et al., 2011, J. Neurosci. 31(39): 13722--13727 Homeostatic interactions at the front of migration control the integrity and the efficiency of a migratory glial chain. [FBrf0216274] Suzanne et al., 2010, Curr. Biol. 20(19): 1773--1778 Coupling of apoptosis and L/R patterning controls stepwise organ looping. [FBrf0212013] Bardet et al., 2008, Proc. Natl. Acad. Sci. U.S.A. 105(37): 13901--13905 A fluorescent reporter of caspase activity for live imaging. [FBrf0205998]

Allele Dmel\thN21G.N22V.Scer\UAS.T:Mmus\Cd8a.T:Disc\RFP-mRFP.T:SV40\nls2.T:Avic\GFP-EGFP

Allele Dmel\th^{N21G.N22V.Scer\UAS.T:Mmus\Cd8a.T:Disc\RFP-mRFP.T:SV40\nls2.T:Avic\GFP-EGFP}