FB2025_01 , released February 20, 2025
Allele: Dmel\LrrkRC.UAS.Tag:FLAG
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General Information
Symbol
Dmel\LrrkRC.UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0246345
Feature type
allele
Associated gene
Dmel\Lrrk
Associated Insertion(s)
Carried in Construct
P{UAS-Lrrk.RC}
Key Links
Genomic Maps

Transgenic product class
missense_variant
(Lee et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Lee et al., 2007)
Mutagen
in vitro construct
(Lee et al., 2007)
Progenitor genotype
Carried in construct
P{UAS-Lrrk.RC}
Cytology
Description

UAS regulatory sequences drive expression of a modified form of Lrrk, carrying a R1069C mutation, tagged at the N-terminal with Tag:FLAG.

(Lee et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Lrrk
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:20,639,575..20,639,577 [+]
Nucleotide change:

AGG20639575TGC

Amino acid change:

R1069C | Lrrk-PB; R1046C | Lrrk-PC; R1137C | Lrrk-PD

Reported amino acid change:

R1069C

Comment:

Analogous R1441C mutation in human LRRK2 implicated in Parkinson's disease 8; mutation carried on in vitro construct; site of nucleotide substitution in fly gene inferred by FlyBase curator based on reported amino acid change.

(Lee et al., 2007)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  Parkinson's disease
      CEA
      (Godena et al., 2014)
      DOES NOT model  Parkinson's disease 8
      CEA
      (Lee et al., 2007)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      This allele represents a human variant implicated in disease.
      (Lee et al., 2007)
      LRRK2:p.Arg1441Cys
      (FlyBase curators, 2019-)
      Variants Synonym(s)
      Associated human disease model(s)
      External database links
      ClinVar: LRRK2_1938
      Comments concerning this variant
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Axonal transport of mitochondria is disrupted in larval motor neurons expressing LrrkRC.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4D42.

      Flies expressing LrrkRC.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4D42 show locomotor defects.

      (Godena et al., 2014)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      LrrkRC.Scer\UAS.T:Zzzz\FLAG
      LrrkRC.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (3)