FB2025_01 , released February 20, 2025
Allele: Dmel\trcK122A.UAS.L.Tag:FLAG
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General Information
Symbol
Dmel\trcK122A.UAS.L.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0246461
Feature type
allele
Associated gene
Dmel\trc
Associated Insertion(s)
Carried in Construct
P{UAS-trc.L.K122A.FLAG}
Key Links
Transgenic product class
missense_variant
(Emoto et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Emoto et al., 2004)
Mutagen
in vitro construct
(He et al., 2005, Emoto et al., 2004)
Progenitor genotype
trcUAS.Tag:FLAG
(Emoto et al., 2004)
Carried in construct
P{UAS-trc.L.K122A.FLAG}
Cytology
Description

Amino acid replacement: K122A.

(He et al., 2005)

UASt regulatory sequences drive expression of a mutated form of the long isoform of trc with the above amino acid substitution. This mutation is at a residue in the active site of the expressed product and therefore should destroy the kinase activity of the enzyme.

(He et al., 2005)

Construct: Amino acid replacement: K122A.

(Emoto et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
trc
Tagged with
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  Parkinson's disease
      modeled by Pink1RNAi.UAS.cYa
      (Wu et al., 2013)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      multidendritic neuron & dendrite, with Scer\GAL4477

      (Emoto et al., 2004)

      multidendritic neuron & dendrite | supernumerary, with Scer\GAL4477

      (Emoto et al., 2004)
      Detailed Description
      Statement
      Reference

      Expression of trcK122A.Scer\UAS under the control of Scer\GAL4ap-md544 does not cause a strong multiple wing hair phenotype.

      (He et al., 2005)

      When trcK122A.Scer\UAS.T:Zzzz\FLAG is driven by Scer\GAL4477 a highly penetrant increase in terminal branches is seen in ddaC dendrites. Tiling defects are also seen.

      (Emoto et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhanced by
      Statement
      Reference

      Scer\GAL4477, trcK122A.UAS.L.Tag:FLAG has multidendritic neuron & dendrite | supernumerary phenotype, non-enhanceable by Rac1N17.UAS, Scer\GAL4477

      (Emoto et al., 2004)

      Scer\GAL4477, trcK122A.UAS.L.Tag:FLAG has multidendritic neuron & dendrite phenotype, non-enhanceable by Rac1N17.UAS, Scer\GAL4477

      (Emoto et al., 2004)
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Scer\GAL4477, trcK122A.UAS.L.Tag:FLAG has multidendritic neuron & dendrite | supernumerary phenotype, non-suppressible by Rac1N17.UAS, Scer\GAL4477

      (Emoto et al., 2004)

      Scer\GAL4477, trcK122A.UAS.L.Tag:FLAG has multidendritic neuron & dendrite phenotype, non-suppressible by Rac1N17.UAS, Scer\GAL4477

      (Emoto et al., 2004)
      Enhancer of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of trcK122A.Scer\UAS.T:Zzzz\FLAG enhances the defects in flight ability caused by expression of Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW.

      The abnormal wing posture phenotype seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is enhanced by co-expression of trcK122A.Scer\UAS.T:Zzzz\FLAG.

      The ability of trcK122A.Scer\UAS.T:Zzzz\FLAG to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is blocked by co-expression of either MarfmiRNA.cUa.Scer\UAS or Atg1Scer\UAS.cSa.

      The ability of trcK122A.Scer\UAS.T:Zzzz\FLAG to enhance the abnormal wing posture phenotype seen in flies expressing Pink1dsRNA.Scer\UAS under the control of Scer\GAL4Mhc.PW is partially suppressed by co-expression of MiroKK102189.

      (Wu et al., 2013)

      trcK122A.Scer\UAS.T:Zzzz\FLAG suppresses the overbranching phenotype seen in trcK122A.Scer\UAS.T:Zzzz\FLAG, Scer\GAL4477 animals but not the tiling defects.

      (Emoto et al., 2004)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      trcK122A.Scer\UAS.L.T:Zzzz\FLAG
      trcK122A.Scer\UAS.T:Zzzz\FLAG
      trcK122A.Scer\UAS
      trcK122A.UAS.L.Tag:FLAG
      trcK122A
      (He et al., 2005)
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0177399
      • FBal0215629
      References (4)