Allele Dmel\trc^{K122A.Scer\UAS.T:Zzzz\FLAG}

General Information
Symbol	Dmel\trc^{K122A.Scer\UAS.T:Zzzz\FLAG}	Species	D. melanogaster
Name		FlyBase ID	FBal0246461
Feature type	allele	Associated gene	Dmel\trc

Allele class
Mutagen	in vitro construct - site directed mutagenesis, in vitro construct - regulatory fusion, in vitro construct - amino acid replacement
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - amino acid replacement (He et al., 2005) in vitro construct - regulatory fusion (He et al., 2005) in vitro construct - site directed mutagenesis (Emoto et al., 2004, He et al., 2005)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	trc^{Scer\UAS.T:Zzzz\FLAG} (Emoto et al., 2004)
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of a mutated form of trc with the above amino acid substitution. This mutation is at a residue in the active site of the expressed product and therefore should destroy the kinase activity of the enzyme. (He et al., 2005) Amino acid replacement: K122A. (He et al., 2005) Construct: Amino acid replacement: K122A. (Emoto et al., 2004)
Carried in construct	P{UAS-trc.K122A} (He et al., 2005, Emoto et al., 2004)
Tagged with	T:Zzzz\FLAG (Emoto et al., 2004)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In
	multidendritic neuron & dendrite, with Scer\GAL4⁴⁷⁷ (Emoto et al., 2004) multidendritic neuron & dendrite \| supernumerary, with Scer\GAL4⁴⁷⁷ (Emoto et al., 2004)
Detailed Description
	Statement Reference When trc^{K122A.Scer\UAS.T:Zzzz\FLAG} is driven by Scer\GAL4⁴⁷⁷ a highly penetrant increase in terminal branches is seen in ddaC dendrites. Tiling defects are also seen. (Emoto et al., 2004)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhanced by
Statement Reference Scer\GAL4⁴⁷⁷, trc^{K122A.Scer\UAS.T:Zzzz\FLAG}/trc^{K122A.Scer\UAS.T:Zzzz\FLAG} has multidendritic neuron & dendrite \| supernumerary phenotype, non-enhanceable by Rac1^N17.Scer\UAS, Scer\GAL4⁴⁷⁷ (Emoto et al., 2004) Scer\GAL4⁴⁷⁷, trc^{K122A.Scer\UAS.T:Zzzz\FLAG}/trc^{K122A.Scer\UAS.T:Zzzz\FLAG} has multidendritic neuron & dendrite phenotype, non-enhanceable by Rac1^N17.Scer\UAS, Scer\GAL4⁴⁷⁷ (Emoto et al., 2004)
Suppressed by
Statement Reference Scer\GAL4⁴⁷⁷, trc^{K122A.Scer\UAS.T:Zzzz\FLAG}/trc^{K122A.Scer\UAS.T:Zzzz\FLAG} has multidendritic neuron & dendrite \| supernumerary phenotype, suppressible by Rac1^N17.Scer\UAS, Scer\GAL4⁴⁷⁷ (Emoto et al., 2004)
NOT suppressed by
Statement Reference Scer\GAL4⁴⁷⁷, trc^{K122A.Scer\UAS.T:Zzzz\FLAG}/trc^{K122A.Scer\UAS.T:Zzzz\FLAG} has multidendritic neuron & dendrite \| supernumerary phenotype, non-suppressible by Rac1^N17.Scer\UAS, Scer\GAL4⁴⁷⁷ (Emoto et al., 2004) Scer\GAL4⁴⁷⁷, trc^{K122A.Scer\UAS.T:Zzzz\FLAG}/trc^{K122A.Scer\UAS.T:Zzzz\FLAG} has multidendritic neuron & dendrite phenotype, non-suppressible by Rac1^N17.Scer\UAS, Scer\GAL4⁴⁷⁷ (Emoto et al., 2004)
Additional Comments
Genetic Interactions
Statement Reference trc^{K122A.Scer\UAS.T:Zzzz\FLAG} suppresses the overbranching phenotype seen in trc^{K122A.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4⁴⁷⁷ animals but not the tiling defects. (Emoto et al., 2004)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to rescue	trc^{K122A.Scer\UAS.T:Zzzz\FLAG}, Scer\GAL4⁴⁷⁷, trc^{K122A.Scer\UAS.T:Zzzz\FLAG} fails to rescue trc¹ (Emoto et al., 2004)
Comments
Stocks ( 1 )
Bloomington	32082 w^*; P{UAS-trc.K122A}2/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	trc^{K122A.Scer\UAS.T:Zzzz\FLAG} trc^{K122A.Scer\UAS} trc^K122A (He et al., 2005)
Name Synonym
Secondary FlyBase IDs
	FBal0177399 FBal0215629
References ( 3 )
Research paper	He et al., 2005, Mol. Biol. Cell 16(2): 689--700 The tricornered Ser/Thr protein kinase is regulated by phosphorylation and interacts with furry during Drosophila wing hair development. [FBrf0184141] Emoto et al., 2004, Cell 119(2): 245--256 Control of dendritic branching and tiling by the Tricornered-kinase/Furry signaling pathway in Drosophila sensory neurons. [FBrf0180024]
Personal communication to FlyBase	Adler, 2010.9.10, Merge of UAS-trc[K122A] transgenes. Merge of UAS-trc[K122A] transgenes. [FBrf0211805]

Allele Dmel\trcK122A.Scer\UAS.T:Zzzz\FLAG

Allele Dmel\trc^{K122A.Scer\UAS.T:Zzzz\FLAG}