Scer\GAL4NP6301 drives expression in the ventral layers of the fan-shaped body, which the authors identify as layers 6-7, respectively.
Scer\GAL4NP6301 labels two s-LNv Pdf neurons. These two s-LNv Pdf neurons are also labeled by expression of sNPF protein. Expression of Scer\GAL4NP6301 was also observed in a small set of Pdf-negative neurons with cell bodies in the eye disc and axons terminating in the larval optic neuropil.
Expression of Scer\GAL4NP6301 is similar in distribution to that seen with sNPF-XP. Scer\GAL4NP6301 appears to be expressed in most of the approximately 600 larval Kenyon cells, and in 190-200 cells of the larval central nervous system, 70 of which are in the brain (including the subesophageal ganglion) and a minimum of 120 in the thoracic and abdominal neuromeres. Three pairs of intensely labelled cells (referred to as LNCs) located dorsally in the protocerebrum are probably neurosecretory cells of the pars lateralis and have axons terminating in the ring gland and anterior aorta. Processes of Scer\GAL4NP6301 immunopositive larval cells are seen in neuropils of the protocerebrum, deutocerebrum, subesophageal ganglion, and most densely in the lobes of the mushroom body. Further processes are seen in the larval optic center, non-glomerular neuropils surrounding the mushroom body lobes, and the pars intercerebralis and lateralis. In the abdominal and thoracic neuromeres of the larval ventral nerve cord, Scer\GAL4NP6301 expressing cells are located dorsally, ventrally and laterally. In larval abdominal neuromeres A7 and A8 there are additional labelled cell bodies with large diameters, and a pair of large dorso-medially located cell bodies expressing Scer\GAL4NP6301 are found in abdominal segment A1. Two bundles of Scer\GAL4NP6301 labelled fibres are found in the ventral ganglia associated with the dors-median and ventro-median tracts. Expression of Scer\GAL4NP6301 in the adult brain generally colocalizes with sNPF protein, though the expression in photoreceptors R7 and R8 is ectopic. Expression of Scer\GAL4NP6301 in the lobula is seen in a set a of columnar neurons with cell bodies in the ventral base of the neuropil and a termination area in the lateral protocerebrum. Expression is strong in the distal lobula.