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General Information
Symbol
Scer\GAL4NP6301
Species
S. cerevisiae
Name
FlyBase ID
FBal0246516
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
snpf-Gal4, NP6301
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference
    Insertion components
    P{GawB}sNPFNP6301
    Product class / Tool use(s)
    Expression Data
    Reporter Expression
    distribution deduced from reporter (Gal4 UAS)
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Additional Information
    Statement
    Reference

    Scer\GAL4NP6301 drives expression in the ventral layers of the fan-shaped body, which the authors identify as layers 6-7, respectively.

    Scer\GAL4NP6301 drives expression in distinct glomeruli in the adult antennal lobe. Except for expression in glomeruli DM2, VA2 and V, labelling is the same as the one seen with sNPF protein.

    Scer\GAL4NP6301 labels two s-LNv Pdf neurons. These two s-LNv Pdf neurons are also labeled by expression of sNPF protein. Expression of Scer\GAL4NP6301 was also observed in a small set of Pdf-negative neurons with cell bodies in the eye disc and axons terminating in the larval optic neuropil.

    Expression of Scer\GAL4NP6301 is similar in distribution to that seen with sNPF-XP. Scer\GAL4NP6301 appears to be expressed in most of the approximately 600 larval Kenyon cells, and in 190-200 cells of the larval central nervous system, 70 of which are in the brain (including the subesophageal ganglion) and a minimum of 120 in the thoracic and abdominal neuromeres. Three pairs of intensely labelled cells (referred to as LNCs) located dorsally in the protocerebrum are probably neurosecretory cells of the pars lateralis and have axons terminating in the ring gland and anterior aorta. Processes of Scer\GAL4NP6301 immunopositive larval cells are seen in neuropils of the protocerebrum, deutocerebrum, subesophageal ganglion, and most densely in the lobes of the mushroom body. Further processes are seen in the larval optic center, non-glomerular neuropils surrounding the mushroom body lobes, and the pars intercerebralis and lateralis. In the abdominal and thoracic neuromeres of the larval ventral nerve cord, Scer\GAL4NP6301 expressing cells are located dorsally, ventrally and laterally. In larval abdominal neuromeres A7 and A8 there are additional labelled cell bodies with large diameters, and a pair of large dorso-medially located cell bodies expressing Scer\GAL4NP6301 are found in abdominal segment A1. Two bundles of Scer\GAL4NP6301 labelled fibres are found in the ventral ganglia associated with the dors-median and ventro-median tracts. Expression of Scer\GAL4NP6301 in the adult brain generally colocalizes with sNPF protein, though the expression in photoreceptors R7 and R8 is ectopic. Expression of Scer\GAL4NP6301 in the lobula is seen in a set a of columnar neurons with cell bodies in the ventral base of the neuropil and a termination area in the lateral protocerebrum. Expression is strong in the distal lobula.

     
    Marker for
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference
    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (2)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (1)
    Reported As
    Symbol Synonym
    Scer\GAL4NP6301
    Name Synonyms
    Secondary FlyBase IDs
      References (9)