FlyBase Allele Report: Dmel\Impβ11[70]

General Information

Symbol

Dmel\Impβ11⁷⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0246520

Feature type

allele

Associated gene

Dmel\Impβ11

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Higashi-Kovtun et al., 2010)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q22term.

(Higashi-Kovtun et al., 2010)

Mutation is predicted to truncate the protein before the Ran-binding domain.

(Higashi-Kovtun et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:15,682,868..15,682,868 [+]

Nucleotide change:

C15682868T

Amino acid change:

Q22term | Impbeta11-PA

Reported amino acid change:

Q22term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Higashi-Kovtun et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology

(Higashi-Kovtun et al., 2010)

abnormal neurophysiology (with Df(2R)ΔM22-103)

(Higashi-Kovtun et al., 2010)

abnormal neurophysiology | somatic clone

(Higashi-Kovtun et al., 2010)

abnormal phototaxis

(Higashi-Kovtun et al., 2010)

lethal - all die before end of P-stage (with Df(2R)ΔM22-103)

(Higashi-Kovtun et al., 2010)

some die during pharate adult stage (with Df(2R)ΔM22-103)

(Higashi-Kovtun et al., 2010)

Phenotype Manifest In

bouton (with Df(2R)ΔM22-103)

(Higashi-Kovtun et al., 2010)

neuromuscular junction (with Df(2R)ΔM22-103)

(Higashi-Kovtun et al., 2010)

Detailed Description

Statement

Reference

Ranbp11⁷⁰ flies exhibit defects in phototaxis and abnormalities in the ERG.

Ranbp11⁷⁰ mutant eye clones lack 'on' and 'off' transients in their ERGs in response to brief exposures to light.

Ranbp11⁷⁰ mutant eyes do not exhibit any gross abnormalities in photoreceptor axon projections: R1-R6 endings appear to terminate appropriately in the lamina, and normal terminals appear in the layers of the medulla that receive the endings of photoreceptors R7 and R8.

Lamina from Ranbp11⁷⁰ mutant animals exhibit a wild-type structure, the R1-R6 photoreceptors appear normal, synapses can be seen with T-bar ribbons, presynaptic densities and clusters of vesicles. Small clear vesicles are normal in size but more numerous at mutant terminals. The cross-sectional area of the terminal profile is also significantly increased, resulting in normal vesicle density. In addition, capitate projections are almost absent from mutant terminals.

Maternal germline clones homozygous for Ranbp11⁷⁰ do not lay eggs.

Although the size of their muscle fibers is normal, Ranbp11⁷⁰/Df(2R)ΔM22-103 mutant larvae exhibit a 30% reduction in bouton number when compared to either control larvae or heterozygote mutant larvae. Ranbp11⁷⁰/Df(2R)ΔM22-103 mutants show a similar 30% decrease in bouton density. Synaptic footprints, a sign that boutons have retracted, were scant in both mutant and control. There is no preferential loss of Ib or Is boutons in Ranbp11⁷⁰/Df(2R)ΔM22-103 neuromuscular junctions, but arbor length and bouton diameter are both slightly decreased when compared to control. The geometry of the synapse is altered in Ranbp11⁷⁰/Df(2R)ΔM22-103 mutants with axonal branches remaining close to the inter-muscular cleft rather than spreading across the width of the muscle.

The amplitude of excitatory junctional potentials (EJPs) in larval muscle 6 is reduced by 40% in Ranbp11⁷⁰/Df(2R)ΔM22-103 mutants. Vesicles also fuse spontaneously at the NMJ, giving rise to quantal events or miniature EJPs. Ranbp11⁷⁰/Df(2R)ΔM22-103 mutants release vesicles at a lower frequency, about 65% less than control. There is also a small, but statistically significant decrease in the amplitude of the mEJPs. Quantal content, or the number of vesicles released per action potential, is reduced by 30% in Ranbp11⁷⁰/Df(2R)ΔM22-103 mutants when corrected for nonlinear summation.

(Higashi-Kovtun et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Impβ11⁷⁰ has abnormal neuroanatomy phenotype, enhanceable by wit^A12/wit[+]

(Higashi-Kovtun et al., 2010)

Impβ11⁷⁰ has abnormal neuroanatomy phenotype, enhanceable by wit[+]/wit^HA1

(Higashi-Kovtun et al., 2010)

NOT Enhanced by

Statement

Reference

Impβ11⁷⁰ has abnormal neuroanatomy phenotype, non-enhanceable by n-syb[+]/nSyb^F33

(Higashi-Kovtun et al., 2010)

Suppressed by

Statement

Reference

Impβ11⁷⁰, wit[+]/wit^HA1 has abnormal neuroanatomy phenotype, suppressible by wit^UAS.cMa/Scer\GAL4^elav.PLu

(Higashi-Kovtun et al., 2010)

Impβ11⁷⁰ has abnormal neurophysiology phenotype, suppressible by sax^{Q263D.UAS.Tag:HA}/Scer\GAL4^RapGAP1-OK6

(Higashi-Kovtun et al., 2010)

NOT suppressed by

Statement

Reference

Impβ11⁷⁰ has abnormal neuroanatomy phenotype, non-suppressible by n-syb[+]/nSyb^F33

(Higashi-Kovtun et al., 2010)

Impβ11⁷⁰ has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^RapGAP1-OK6/tkv^{Q253D.UAS.cNb}

(Higashi-Kovtun et al., 2010)

Impβ11⁷⁰ has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^G14/tkv^{Q253D.UAS.cNb}

(Higashi-Kovtun et al., 2010)

Impβ11⁷⁰ has abnormal neurophysiology phenotype, non-suppressible by Scer\GAL4^G14/sax^{Q263D.UAS.Tag:HA}

(Higashi-Kovtun et al., 2010)

Enhancer of

Statement

Reference

Impβ11⁷⁰ is an enhancer of abnormal neuroanatomy phenotype of wit^A12

(Higashi-Kovtun et al., 2010)

Impβ11⁷⁰ is an enhancer of abnormal neuroanatomy phenotype of wit^HA1

(Higashi-Kovtun et al., 2010)

NOT Enhancer of

Statement

Reference

Ranbp11[+]/Impβ11⁷⁰ is a non-enhancer of abnormal neuroanatomy phenotype of nSyb^F33

(Higashi-Kovtun et al., 2010)

NOT Suppressor of

Statement

Reference

Ranbp11[+]/Impβ11⁷⁰ is a non-suppressor of abnormal neuroanatomy phenotype of nSyb^F33

(Higashi-Kovtun et al., 2010)

Other

Statement

Reference

Impβ11⁷⁰, Mad¹⁰ has abnormal neuroanatomy phenotype

(Higashi-Kovtun et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Impβ11⁷⁰ has bouton phenotype, enhanceable by wit^A12/wit[+]

(Higashi-Kovtun et al., 2010)

Impβ11⁷⁰ has bouton phenotype, enhanceable by wit[+]/wit^HA1

(Higashi-Kovtun et al., 2010)

NOT Enhanced by

Statement

Reference

Impβ11⁷⁰ has bouton phenotype, non-enhanceable by n-syb[+]/nSyb^F33

(Higashi-Kovtun et al., 2010)

Suppressed by

Statement

Reference

Impβ11⁷⁰ has bouton phenotype, suppressible by Scer\GAL4^RapGAP1-OK6/tkv^{Q253D.UAS.cNb}

(Higashi-Kovtun et al., 2010)

Impβ11⁷⁰ has bouton phenotype, suppressible by sax^{Q263D.UAS.Tag:HA}/Scer\GAL4^RapGAP1-OK6

(Higashi-Kovtun et al., 2010)

Impβ11⁷⁰, wit[+]/wit^HA1 has bouton phenotype, suppressible by wit^UAS.cMa/Scer\GAL4^elav.PLu

(Higashi-Kovtun et al., 2010)

NOT suppressed by

Statement

Reference

Impβ11⁷⁰ has bouton phenotype, non-suppressible by n-syb[+]/nSyb^F33

(Higashi-Kovtun et al., 2010)

Impβ11⁷⁰ has bouton phenotype, non-suppressible by Scer\GAL4^G14/tkv^{Q253D.UAS.cNb}

(Higashi-Kovtun et al., 2010)

Impβ11⁷⁰ has bouton phenotype, non-suppressible by Scer\GAL4^G14/sax^{Q263D.UAS.Tag:HA}

(Higashi-Kovtun et al., 2010)

Enhancer of

Statement

Reference

Ranbp11[+]/Impβ11⁷⁰ is an enhancer of bouton phenotype of wit^A12

(Higashi-Kovtun et al., 2010)

Ranbp11[+]/Impβ11⁷⁰ is an enhancer of bouton phenotype of wit^HA1

(Higashi-Kovtun et al., 2010)

NOT Enhancer of

Statement

Reference

Ranbp11[+]/Impβ11⁷⁰ is a non-enhancer of bouton phenotype of nSyb^F33

(Higashi-Kovtun et al., 2010)

NOT Suppressor of

Statement

Reference

Ranbp11[+]/Impβ11⁷⁰ is a non-suppressor of bouton phenotype of nSyb^F33

(Higashi-Kovtun et al., 2010)

Other

Statement

Reference

Impβ11⁷⁰, Mad¹⁰ has bouton phenotype

(Higashi-Kovtun et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Ranbp11⁷⁰/Mad¹⁰ double mutants exhibit a 15% reduction in synaptic bouton number.

Ranbp11⁷⁰/+; wit^A12/+ double heterozygous larvae exhibit significantly fewer boutons than either wild-type or each heterozygous allele alone.

Ranbp11⁷⁰/+; wit^HA1/+ double heterozygous larvae exhibit significantly fewer boutons than either wild-type or each heterozygous allele alone.

Larvae heterozygous for Ranbp11⁷⁰ and n-syb^F33 exhibit normal synaptic arbors.

Neuronal expression of wit^Scer\UAS.cMa (under the control of Scer\GAL4^elav.PLu reverses the reduction in bouton number in Ranbp11⁷⁰/+ ; wit^HA1/+ larvae.

Expression of tkv^{Q253D.Scer\UAS.cNb} in motor neurons (under the control of Scer\GAL4^OK6) rescues bouton number and EJP phenotypes in Ranbp11⁷⁰ mutants, although mEJP frequency is not rescued.

Expression of tkv^{Q253D.Scer\UAS.cNb} in muscle (under the control of Scer\GAL4^G14) does not rescue bouton number or mEJP and EJP phenotypes in Ranbp11⁷⁰ mutants.

Expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} in motor neurons (under the control of Scer\GAL4^OK6) rescues bouton number and EJP phenotypes in Ranbp11⁷⁰ mutants, although mEJP frequency is not rescued.

Expression of sax^{Q263D.Scer\UAS.T:Ivir\HA1} in muscle (under the control of Scer\GAL4^G14) does not rescue bouton number or mEJP and EJP phenotypes in Ranbp11⁷⁰ mutants.

(Higashi-Kovtun et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Impβ11⁷⁰ is rescued by Scer\GAL4^ey.PH/Impβ11^UAS.EGFP

(Higashi-Kovtun et al., 2010)

Impβ11⁷⁰ is rescued by Scer\GAL4^elav.PLu/Impβ11^UAS.EGFP

(Higashi-Kovtun et al., 2010)

Impβ11⁷⁰ is rescued by Scer\GAL4^da.G32/Impβ11^UAS.EGFP

(Higashi-Kovtun et al., 2010)

Not rescued by

Impβ11⁷⁰ is not rescued by Scer\GAL4^G14/Impβ11^UAS.EGFP

(Higashi-Kovtun et al., 2010)

Comments

Expression of Ranbp11^{Scer\UAS.T:Avic\GFP-EGFP} in photoreceptors under the control of Scer\GAL4^ey.PH fully restores the on and off transients in Ranbp11⁷⁰ mutant eye clones.

Expression of Ranbp11^{Scer\UAS.T:Avic\GFP-EGFP} in all tissues (with a Scer\GAL4^da.G32 driver) or in neurons (with a Scer\GAL4^elav.PLu driver) rescues the reduction in bouton number/muscle surface area in Ranbp11⁷⁰ mutants. Expression of Ranbp11^{Scer\UAS.T:Avic\GFP-EGFP} in muscle (with a Scer\GAL4^G14 driver) does not rescue this phenotype in Ranbp11⁷⁰ mutants.

The observed defects in quantal content and EJP amplitude in Ranbp11⁷⁰ are fully rescued upon expression of Ranbp11^{Scer\UAS.T:Avic\GFP-EGFP} in the nervous system under the control of Scer\GAL4^elav.PLu. Although quantal content is somewhat increased by transgene expression in muscle (under the control of Scer\GAL4^G14), it does not correspond to a statistically significant restoration of EJP amplitude. Separately, neither neuronal nor muscle expression of Ranbp11^{Scer\UAS.T:Avic\GFP-EGFP} is adequate to restore mEJP frequency or amplitude to control levels; however, concurrent expression in both neurons and muscle significantly increases mini frequency. mEJP amplitude is not rescued.

(Higashi-Kovtun et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Impβ11⁷⁰

Ranbp11⁷⁰

imp-β11⁷⁰

(Restrepo et al., 2022)

impβ11⁷⁰

(Sulkowski et al., 2016, Higashi-Kovtun et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (3)

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