FB2025_01 , released February 20, 2025
Allele: Dmel\Mdh2Tag:MYC
Open Close
General Information
Symbol
Dmel\Mdh2Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0246555
Feature type
allele
Associated gene
Dmel\Mdh2
Associated Insertion(s)
Carried in Construct
P{Mdh2-myc.W}
Also Known As
Mdh2-myc
Key Links
Genomic Maps

Transgenic product class
genomic_DNA
(Wang et al., 2010)
wild_type
(Wang et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Wang et al., 2010)
wild_type
(Wang et al., 2010)
Mutagen
in vitro construct
(Wang et al., 2010)
Progenitor genotype
Carried in construct
P{Mdh2-myc.W}
Cytology
Description

A 5.7 kb fragment encompassing the entire Mdh2 locus, including approximately 1.1 kb upstream of the transcription start site and approximately 2.3 kb downstream of the transcription stop site, using the genomic clone BACR27G04 as the template, and including a tandem Tag:MYC tag at the 3' end of the Mdh2 coding region.

(Wang et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
Mdh2
Mdh2_Myc-tag-5.7kb
Encoded product / tool
Mdh2
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
rescue_region
3R:18,225,299..18,231,052 [+]
Comment:

A 5.7 kb fragment encompassing the entire Mdh2 locus, including approximately 1.1 kb upstream of the transcription start site and approximately 2.3 kb downstream of the transcription stop site, with a tandem T:Hsap \MYC tag inserted at the 3' end of the Mdh2 coding region.

(Wang et al., 2010)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter or direct label
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      wandering third instar larval stage
      embryonic/larval salivary gland
      (Wang et al., 2010)
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Mdh2
      (Wang et al., 2010)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Mdh2Tag:MYC rescues Mdh22/Mdh21

      (Wang et al., 2010)
      Comments

      One copy of Mdh2T:Hsap\MYC restores normal salivary gland cell death and efficiently rescues pupal lethality in Mdh21/Mdh22 animals.

      (Wang et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Mdh2T:Hsap\MYC
      Mdh2Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (3)