Expression of shits.Scer\UAS.cUa under the control of Scer\GAL4upd-E132 at 29[o]C (restrictive temperature) lengthens the period of locomotor activity by approximately 0.7 hours under constant darkness conditions. Locomotor activity rhythms revert back to normal after the flies are shifted to 21[o]C (permissive temperature).
Animals carrying shits.Scer\UAS.cUa and Scer\GAL4repo.PU raised at 18[o]C and then subsequently shifted as adults to 30[o]C show 100% lethality within 3 days of the temperature shift.
Expression of shits.Scer\UAS.cUa under the control of Scer\GAL4Mz709 at 30[o]C strongly suppresses glial clearance of axonal debris from the central nervous system after severing of olfactory receptor neurons. Expression of shits.Scer\UAS.cUa under the control of Scer\GAL4alrm.PD at 30[o]C has no effect on axon debris clearance after severing of olfactory receptor neurons.
Animals carrying shits.Scer\UAS.cUa expressed under the control of Scer\GAL4en-e16E and shifted to the restrictive temperature of 31[o]C at the third larval instar show a dramatic induction of wing hairs. If the shift to the restrictive temperature occurs at 26 hours after puparium formation, a milder wing-hair duplication phenotype is seen in the resulting adults.