Allele Dmel\Nlg1^I960

General Information
Symbol	Dmel\Nlg1^I960	Species	D. melanogaster
Name		FlyBase ID	FBal0246573
Feature type	allele	Associated gene	Dmel\Nlg1

Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Banovic et al., 2010)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: K242@. (Banovic et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| larval stage (Banovic et al., 2010) neuroanatomy defective \| larval stage (with Df(3R)dsx29) (Banovic et al., 2010) neuroanatomy defective \| larval stage (with Nlg1^ex2.3) (Banovic et al., 2010, Mosca et al., 2012) neuroanatomy defective \| larval stage (with Nlg1^H324) (Banovic et al., 2010) neurophysiology defective \| larval stage (with Nlg1^H324) (Banovic et al., 2010) viable (with Nlg1^ex2.3) (Mosca et al., 2012)
Phenotype Manifest In
	neuromuscular junction \| larval stage (Banovic et al., 2010) neuromuscular junction \| larval stage (with Nlg1^ex2.3) (Banovic et al., 2010) neuromuscular junction \| larval stage (with Nlg1^H324) (Banovic et al., 2010) NMJ bouton (with Nlg1^ex2.3) (Mosca et al., 2012) NMJ bouton \| larval stage (Banovic et al., 2010) NMJ bouton \| larval stage (with Df(3R)dsx29) (Banovic et al., 2010) NMJ bouton \| larval stage (with Nlg1^ex2.3) (Banovic et al., 2010) NMJ bouton \| larval stage (with Nlg1^H324) (Banovic et al., 2010) subsynaptic reticulum (with Nlg1^H324) (Banovic et al., 2010)
Detailed Description
	Statement Reference Mutants have smaller neuromuscular junctions than normal. The percentage of non-growing branches at the neuromuscular junction is increased compared to controls at both the first and third larval instar stages. The neuromuscular junctions of Nlg1[H324]/Nlg1[I960] and Nlg1[ex2.3]/Nlg1[I960] larvae are smaller than normal. The average bouton number per muscle area and average number of boutons normalized to synaptic branch length are significantly reduced, and the average diameter of the largest bouton at a given neuromuscular junction is slightly but significantly increased in homozygous, Nlg1[I960]/Nlg1[H324], Nlg1[I960]/Df(3R)dsx29 and Nlg1[I960]/Nlg1[ex2.3] mutants. Ultrastructurally, presynaptic active zones are still formed in Nlg1[H324]/Nlg1[I960] mutant boutons. The mutant active zones contain T-bars and clustered synaptic vesicles. The synaptic vesicles are present at approximately normal size and density, with large vesicle diameters in slightly higher numbers than normal. A subset of the mutant boutons show a reduction in thickness of the subsynaptic reticulum (SSR). The relative SSR area is significantly reduced in the mutant boutons compared to controls, and in extreme cases, boutons appear to be in "direct contact" with the contractile muscle filaments without being surrounded by SSR. Active zones are still present at locations lacking SSR and maintain the tight apposition of presynaptic and postsynaptic membranes. The number of membrane ruffles in active zones is strongly increased in the mutants, while the distance of these ruffles to the centre of the T-bar is unchanged. The mean amplitude of the evoked excitatory junctional current (EJC) from neuromuscular junctions of Nlg1[H324]/Nlg1[I960] third instar larvae is significantly reduced compared to controls at both 0.5mM and 1mM extracellular Ca[2+]. The mean amplitude of spontaneous miniature EJCs at the mutant neuromuscular junction is unchanged relative to wild type. (Banovic et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Df(1)Ten-a, Nlg1^I960/Nlg1^ex2.3 has lethal \| larval stage phenotype (Mosca et al., 2012)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	Nlg1^I960/Nlg1^H324 is rescued by Scer\GAL4^Mef2.PR/Nlg1^{Scer\UAS.T:Avic\GFP-EGFP} (Banovic et al., 2010)
Not rescued by	Nlg1^I960/Nlg1^H324 is not rescued by Scer\GAL4^elav.PU/Nlg1^{Scer\UAS.T:Avic\GFP-EGFP} (Banovic et al., 2010) Nlg1^I960/Nlg1^H324 is not rescued by Scer\GAL4^Mef2.PR/Nlg1^{Δextra.Scer\UAS.T:Hsap\MYC,T:Avic\GFP-EGFP} (Banovic et al., 2010)
Comments	Expression of Nlg1[Scer\UAS.T:Avic\GFP-EGFP] under the control of Scer\GAL4[Mef2.PR], but not under the control of Scer\GAL4[elav.PU], rescues the neuromuscular junction defects of Nlg1[I960]/Nlg1[H324] larvae. Expression of Nlg1[Δextra.Scer\UAS.T:Hsap\MYC,T:Avic\GFP-EGFP] under the control of Scer\GAL4[Mef2.PR] does not rescue the neuromuscular junction defects of Nlg1[I960]/Nlg1[H324] larvae. (Banovic et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	dnlg1^I960 (Banovic et al., 2010) I960 (Mosca et al., 2012) Nlg1^I960
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Mosca et al., 2012, Nature 484(7393): 237--241 Trans-synaptic Teneurin signalling in neuromuscular synapse organization and target choice. [FBrf0218053] Banovic et al., 2010, Neuron 66(5): 724--738 Drosophila Neuroligin 1 Promotes Growth and Postsynaptic Differentiation at Glutamatergic Neuromuscular Junctions. [FBrf0211035]
Supplementary material	Mosca et al., 2012, Nature 484(7393): Online methods. [FBrf0219881]

Allele Dmel\Nlg1I960

Allele Dmel\Nlg1^I960