Allele Dmel\Rabex-5^VDRC.cUa

General Information
Symbol	Dmel\Rabex-5^VDRC.cUa	Species	D. melanogaster
Name		FlyBase ID	FBal0246621
Feature type	allele	Associated gene	Dmel\Rabex-5

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - RNAi
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Yan et al., 2010) in vitro construct - RNAi (Yan et al., 2010)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an RNAi construct targeting Rabex-5. (Yan et al., 2010)
Carried in construct	P{VDRC.Rabex-5.U} (Yan et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	large body, with Scer\GAL4^Act.PU (Yan et al., 2010) lethal \| larval stage, with Scer\GAL4^unspecified (Yan et al., 2010) lethal \| P-stage, with Scer\GAL4^unspecified (Yan et al., 2010) melanotic mass phenotype \| larval stage, with Scer\GAL4^unspecified (Yan et al., 2010) melanotic mass phenotype \| P-stage, with Scer\GAL4^unspecified (Yan et al., 2010) visible, with Rabex-5^ex42, Scer\GAL4^C-765 (Yan et al., 2010) visible, with Scer\GAL4^Act.PU (Yan et al., 2010) visible, with Scer\GAL4^C-765 (Yan et al., 2010)
Phenotype Manifest In
	posterior crossvein, with Rabex-5^ex42, Scer\GAL4^C-765 (Yan et al., 2010) posterior crossvein, with Scer\GAL4^Act.PU (Yan et al., 2010) posterior crossvein, with Scer\GAL4^C-765 (Yan et al., 2010) wing, with Scer\GAL4^Act.PU (Yan et al., 2010) wing vein L2, with Rabex-5^ex42, Scer\GAL4^C-765 (Yan et al., 2010) wing vein L2, with Scer\GAL4^Act.PU (Yan et al., 2010) wing vein L2, with Scer\GAL4^C-765 (Yan et al., 2010)
Detailed Description
	Statement Reference Larvae and pupae expressing Rabex-5[VDRC.cUa] driven by strong, constitutive drivers die as giant larvae or giant pupae, often containing melanotic tumors. Expression of Rabex-5[VDRC.cUa] driven by Scer\GAL4[Act.PU] results in viable adults with about a 10% increase in length, weight and wing size compared with controls. Expression of Rabex-5[VDRC.cUa] driven by Scer\GAL4[Act.PU] or Scer\GAL4[C-765] results in extra posterior crossveins and extra longitudinal veins compared to controls. Rabex-5[ex42]/+ enhances the wing phenotype resulting from Rabex-5[VDRC.cUa] expression driven by Scer\GAL4[C-765]. (Yan et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Dcr-2^Scer\UAS.cDa, Rabex-5^VDRC.cUa, Scer\GAL4^ey.PU has visible phenotype, suppressible \| partially by Ras85D^e1B/Ras85D[+] (Yan et al., 2010) Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has large body phenotype, suppressible \| partially by Ras85D^e1B/Ras85D[+], Scer\GAL4^Act.PU (Yan et al., 2010) Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has visible phenotype, suppressible by Ras85D^e1B/Ras85D[+], Scer\GAL4^Act.PU (Yan et al., 2010) Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has visible phenotype, suppressible by Ras85D^e2F/Ras85D[+], Scer\GAL4^Act.PU (Yan et al., 2010) Rabex-5^VDRC.cUa, Scer\GAL4^C-765 has visible phenotype, suppressible by Ras85D^e1B/Ras85D[+], Scer\GAL4^C-765 (Yan et al., 2010)
Enhancer of
Statement Reference Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Egfr^{U.A887T.Scer\UAS}, Scer\GAL4^GMR.PU (Yan et al., 2010) Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU is an enhancer of visible phenotype of Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^GMR.PU (Yan et al., 2010)
NOT Enhancer of
Statement Reference Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PU, phl^{Scer\UAS.F179} (Yan et al., 2010)
Other
Statement Reference Dcr-2^Scer\UAS.cDa, Rabex-5^VDRC.cUa, Scer\GAL4^ey.PU has visible phenotype (Yan et al., 2010) Egfr^{U.A887T.Scer\UAS}, Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU has lethal phenotype (Yan et al., 2010) Rabex-5^VDRC.cUa, Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^GMR.PU has lethal phenotype (Yan et al., 2010)
Phenotype Manifest In
Suppressed by
Statement Reference Dcr-2^Scer\UAS.cDa, Rabex-5^VDRC.cUa, Scer\GAL4^ey.PU has eye phenotype, suppressible \| partially by Ras85D^e1B/Ras85D[+] (Yan et al., 2010) Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has posterior crossvein phenotype, suppressible by Ras85D^e1B/Ras85D[+] (Yan et al., 2010) Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has posterior crossvein phenotype, suppressible by Ras85D^e2F/Ras85D[+] (Yan et al., 2010) Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has wing phenotype, suppressible \| partially by Ras85D^e1B/Ras85D[+], Scer\GAL4^Act.PU (Yan et al., 2010) Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has wing vein L2 phenotype, suppressible by Ras85D^e1B/Ras85D[+] (Yan et al., 2010) Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU has wing vein L2 phenotype, suppressible by Ras85D^e2F/Ras85D[+] (Yan et al., 2010) Rabex-5^VDRC.cUa, Scer\GAL4^C-765 has posterior crossvein phenotype, suppressible by Ras85D^e1B/Ras85D[+] (Yan et al., 2010) Rabex-5^VDRC.cUa, Scer\GAL4^C-765 has wing vein L2 phenotype, suppressible by Ras85D^e1B/Ras85D[+] (Yan et al., 2010)
Enhancer of
Statement Reference Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Egfr^{U.A887T.Scer\UAS}, Scer\GAL4^GMR.PU (Yan et al., 2010) Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^GMR.PU (Yan et al., 2010)
NOT Enhancer of
Statement Reference Rabex-5^VDRC.cUa, Scer\GAL4^GMR.PU is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PU, phl^{Scer\UAS.F179} (Yan et al., 2010)
Other
Statement Reference Dcr-2^Scer\UAS.cDa, Rabex-5^VDRC.cUa, Scer\GAL4^ey.PU has antenna phenotype (Yan et al., 2010) Dcr-2^Scer\UAS.cDa, Rabex-5^VDRC.cUa, Scer\GAL4^ey.PU has eye phenotype (Yan et al., 2010) Rabex-5^VDRC.cUa, Ras85D^{V12.S35.Scer\UAS}, Scer\GAL4^GMR.PU has antenna phenotype (Yan et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Co-expression of Rabex-5[VDRC.cUa] with Dcr-2[Scer\UAS.cDa] in the early eye driven by Scer\GAL4[ey.PU] leads to a fraction of the eyes with severe phenotypes, including overgrown eyes, outgrowths, small or lost eyes, and extra antennae. Ras85D[e1B] and Ras85D[e2F] dominantly suppress the extra wing veins caused by constitutive expression of Rabex-5[VDRC.cUa] via Scer\GAL4[Act.PU]. Ras85D[e1B] dominantly suppresses the extra wing veins caused by wing-specific expression of Rabex-5[VDRC.cUa] via Scer\GAL4[C-765]. A Ras85D[e1B]/+ genetic background results in fewer flies with severe eye phenotypes upon co-expression of Rabex-5[VDRC.cUa] and Dcr-2[Scer\UAS.cDa] driven by Scer\GAL4[ey.PU]. Ras85D[e1B] dominantly suppresses the increased body size, body length and wing area resulting from the expression of Rabex-5[VDRC.cUa] driven by Scer\GAL4[Act.PU]. Body weight of all flies and wing area in female flies are no longer statistically different from controls. Co-expression of Rabex-5[VDRC.cUa] significantly enhances the number of eyes with black tissue and significantly increases lethality resulting from the expression of Ras85D[V12.S35.Scer\UAS] driven by Scer\GAL4[GMR.PU]. The overgrowth, extra antennae, and ectopic eye phenotypes of Ras85D[V12.S35.Scer\UAS] overexpression are also enhanced by Rabex-5[VDRC.cUa]-expression. Co-expression of Rabex-5[VDRC.cUa] significantly enhances the number of eyes with black tissue and significantly increases lethality resulting from the expression of Egfr[U.A887T.Scer\UAS] driven by Scer\GAL4[GMR.PU]. Co-expression of Rabex-5[VDRC.cUa] fails to enhance the eye phenotypes resulting from the overexpression of phl[Scer\UAS.F179] driven by Scer\GAL4[GMR.PU]. (Yan et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	This construct is originally from the Vienna Drosophila RNAi Center (VDRC). (Yan et al., 2010)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Rabex-5^VDRC.cUa
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Yan et al., 2010, Curr. Biol. 20(15): 1378--1382 Rabex-5 Ubiquitin Ligase Activity Restricts Ras Signaling to Establish Pathway Homeostasis in Drosophila. [FBrf0211477]

Allele Dmel\Rabex-5VDRC.cUa

Allele Dmel\Rabex-5^VDRC.cUa