FlyBase Allele Report: Dmel\mars[91]

General Information

Symbol

Dmel\mars⁹¹

Species

D. melanogaster

Name

FlyBase ID

FBal0246631

Feature type

allele

Associated gene

Dmel\mars

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Zhang et al., 2009)

Mutagen

Delta2-3 transposase

(Zhang et al., 2009)

Progenitor genotype

P{EP}drk^EP2477

(Zhang et al., 2009)

Cytology

Description

Imprecise excision of the progenitor insertion results in a deletion which removes 531 bp of the first exon of mars, including the start codon.

(Zhang et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:13,503,016..13,503,546 [+]

Comment:

531bp deletion resulting from imprecise excision of P{EP}drk^EP2477

(Zhang et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Zhang et al., 2013)

abnormal mitotic cell cycle | conditional

(Zhang et al., 2009)

chemical sensitive

(Zhang et al., 2013)

fertile

(Zhang et al., 2009)

fertile (with Df(2R)CX1)

(Zhang et al., 2009)

grandchildless | partially

(Zhang et al., 2009)

grandchildless | partially (with Df(2R)CX1)

(Zhang et al., 2009)

viable

(Zhang et al., 2009)

viable (with Df(2R)CX1)

(Zhang et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

When treated with a low concentration of demecolcine, which partially depolymerises microtubles from mitotic spindles, approximately 50% of mars⁹¹ mutant embryos completely lose microtubules from around the chromosomes, while the remaining embryos exhibit much weaker staining of the microtubules. mars⁹¹ mutants are less resistant to demecolcine microtubule depolymerisation.

mars⁹¹ embryonic cells exhibit dissociation of centrosomes from mitotic spindles. The mitotic spindles without centrosomes tend to collapse into monopolar spindles.

(Zhang et al., 2013)

90.8% of embryos from homozygous mars⁹¹ parents die during embryogenesis, with the majority of embryos displaying defects in cellularisation. 9.2% hatch into larvae, but only 5.5% of embryos survive to adulthood. Embryos from mars⁹¹ homozygous parents display severe mitotic defects during the rapid nuclear divisions in early syncytial blastoderm stage embryos. Centrosomes frequently detach from spindles and from the nuclear envelope and nucleate astral microtubules in ectopic positions.

mars⁹¹/Df(2R)CX1 animals are viable and fertile. Offspring of mars⁹¹/Df(2R)CX1 have severe embryonic defects and the majority die during embryogenesis.

(Zhang et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mars⁹¹ is rescued by mars^UASp.N.GFP

(Zhang et al., 2013)

mars⁹¹ is rescued by mars^UASp.EGFP/Scer\GAL4^{VP16.mat.αTub67C}

(Zhang et al., 2009)

Not rescued by

mars⁹¹ is not rescued by Scer\GAL4^da.G32/mars^{1-430.UASp.N.GFP}

(Zhang et al., 2013)

mars⁹¹ is not rescued by mars^{431-921.UASp.N.GFP}

(Zhang et al., 2013)

Comments

Expression of mars^{1-430.Scer\UAS.P\T.N.T:Avic\GFP} fails to rescue the lethality associated with mars⁹¹ mutants but is able to largely maintain the mitotic spindle morphology in the absence of endogenous mars.

(Zhang et al., 2013)

Expression of mars^{Scer\UAS.P\T.T:Avic\GFP-EGFP} under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} fully rescues the embryogenesis defects in offspring from mars⁹¹ homozygous parents.

(Zhang et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mars⁹¹

(Zhang et al., 2013, Zhang et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (2)

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