Allele Dmel\ST6Gal^L22

General Information
Symbol	Dmel\ST6Gal^L22	Species	D. melanogaster
Name		FlyBase ID	FBal0246662
Feature type	allele	Associated gene	Dmel\ST6Gal

Allele class
Mutagen	recombination, ends-out gene targeting
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ends-out gene targeting (Repnikova et al., 2010) recombination (Repnikova et al., 2010)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Generated by ends-out targeting of the ST6Gal locus, resulting in a deletion that removes most of the coding sequence. The design of the targeting construct used to generate this allele was aimed at creating a mutant allele with Avic\GFP expression driven by the ST6Gal promoter (by inserting the Avic\GFP coding sequence), however the Avic\GFP coding sequence was damaged by a short insertion of approximately 30bp during the targeting event, and there is no Avic\GFP expression in the mutant. (Repnikova et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| larval stage (Repnikova et al., 2010)
Phenotype Manifest In
	embryonic/larval neuromuscular junction (Repnikova et al., 2010) NMJ bouton \| larval stage (Repnikova et al., 2010)
Detailed Description
	Statement Reference Mutant larvae have a decreased number of muscle synaptic branches and boutons at the neuromuscular junction of the dorsal abdominal muscle 1 (which is innervated by motor neuron MN1-Ib). (Repnikova et al., 2010)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments	ST6Gal[t8] rescues the locomotor defects of ST6Gal[S23] larvae and rescues the shortened longevity, bang sensitivity and temperature sensitive paralytic phenotype of ST6Gal[S23] adults. The reduced number of boutons at the neuromuscular junction of dorsal abdominal muscle 1 which is seen in ST6Gal[S23] larvae is partially rescued by ST6Gal[t8]. (Repnikova et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	L22 (Repnikova et al., 2010) ST6Gal^L22
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Repnikova et al., 2010, J. Neurosci. 30(18): 6466--6476 Sialyltransferase regulates nervous system function in Drosophila. [FBrf0210736]

Allele Dmel\ST6GalL22

Allele Dmel\ST6Gal^L22