Allele Dmel\metro⁴⁷

General Information
Symbol	Dmel\metro⁴⁷	Species	D. melanogaster
Name		FlyBase ID	FBal0246670
Feature type	allele	Associated gene	Dmel\metro
Also Known As	metro^*47

Allele class
Mutagen	P-element activity
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	P-element activity (Bachmann et al., 2010)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	P{GT1}metro^BG02148 (Bachmann et al., 2010)
Nature of the lesion	Statement Reference Imprecise excision of the progenitor insertion, resulting in a deletion that lacks exons 2-5 and part of exon 6 of metro. (Bachmann et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	fertile (Bachmann et al., 2010) neuroanatomy defective (Bachmann et al., 2010) viable (Bachmann et al., 2010)
Phenotype Manifest In
	neuromuscular junction (Bachmann et al., 2010)
Detailed Description
	Statement Reference Mutants show a 35% reduction in the number of type Ib boutons/muscle surface area at the neuromuscular junction (NMJ) compared to controls. There is also an increased frequency of extra large and irregularly shaped boutons at the mutant NMJs compared to controls. Ultrastructurally, synaptic contacts at mutant boutons appear largely normal with respect to electron density and the presence of presynaptic T-bars. The length of individual electron dense zones observed on single sections of mutant boutons is mostly within the range seen in controls. However, tightly juxtaposed electron dense zones are frequently observed on sections of mutant boutons. The mean surface area of receptor fields is considerably increase in homozygous and hemizygous mutants compared to controls. The frequency and amplitude of spontaneous miniature excitatory junctional currents at the mutant neuromuscular junction is not significantly different from that of controls. The amplitude of excitatory junctional currents evoked by low-frequency stimulation of motoneurons is not significantly different from that of controls. (Bachmann et al., 2010)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	metro⁴⁷ is rescued by Scer\GAL4^αTub84B.PL/metro^B.Scer\UAS (Bachmann et al., 2010)
Comments	Expression of metro[B.Scer\UAS] under the control of Scer\GAL4[αTub84B.PL] rescues the reduction in bouton number and increase in bouton size which is seen at the neuromuscular junction in metro[47] mutants. (Bachmann et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	metro⁴⁷ metro^*47 (Bachmann et al., 2010, Bachmann et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Bachmann et al., 2010, J. Neurosci. 30(17): 5811--5824 A perisynaptic ménage à trois between Dlg, DLin-7, and Metro controls proper organization of Drosophila synaptic junctions. [FBrf0210640]
Supplementary material	Bachmann et al., 2010, J. Neurosci. 30(17): Supplementary material. [FBrf0212098]

Allele Dmel\metro47

Allele Dmel\metro⁴⁷