Imprecise excision of the progenitor insertion, resulting in a 357bp deletion starting at position -33. The deletion removes all of exon 1, the intervening intron and part of exon 2.
357 bp deletion resulting from the imprecise excision of P{EPgy2}Mob4EY23407. Reported as extending into the gene from the insertion site 33bp upstream of the Mob4 AUG.
Less than 10% of homozygotes and Mob4EYΔL3/Df(2R)42 animals survive to the third larval instar, most die as first or second instar larvae.
15% of Mob4EYΔL3/Mob4EY23407 animals develop to the third larval instar, 3% pupate but do not eclose.
Mob4EYΔL307/Mob4EYΔL3 adults have a significantly reduced lifespan compared to controls.
Third instar mutant larvae have a "tail-flip" phenotype.
Mob4EYΔL3/Df(2R)42 and Mob4EYΔL3/Mob4EY23407 third instar larvae have supernumerary boutons at the neuromuscular junctions of muscles 6/7 and 4 compared to controls.
Ultrastructurally, multiple abnormal cisterna-like endocytic structures are seen budding from the plasma membrane of presynaptic terminals at the neuromuscular junctions of Mob4EYΔL3/Df(2R)42 larvae. In peripheral nerves of these animals, accumulations of microtubule-associated vesicles with larger diameters than in controls are seen.
Mob4EYΔL3 third larval instar neuromuscular junctions show a small (approximately 15%) reduction in evoked EPSC amplitude (measured at low stimulation frequency in 2mM extracellular Ca[2+]). The mutants show relatively normal synaptic depression during 10 Hz stimulation but complete failure in EPSCs are seen after 4 seconds with a faster 50Hz stimulation train.
Mutants have abnormal microtubule morphology at the neuromuscular junction and in peripheral nerves and muscles.
Mob4EYΔL3 has lethal | larval stage phenotype, suppressible by Scer\GAL4Act.PU/Hsap\MOB41.UAS
Expression of Hsap\MOBKL31.Scer\UAS under the control of Scer\GAL4Act.PU rescues the larval lethality of Mob4EYΔL3 homozygotes; the rescued animals being able to pupate and eclose as adults.
Mob4EYΔL3 is rescued by Scer\GAL4Act.PU/Mob4UAS.N.mCherry
Mob4EYΔL3 is rescued by Mob4UAS.N.mCherry/Scer\GAL4Tub.PU
Mob4EYΔL3 is rescued by Scer\GAL4Act.PU/Mob4UAS.C.mCherry
Mob4EYΔL3 is rescued by Scer\GAL4Tub.PU/Mob4UAS.C.mCherry
Mob4EYΔL3 is rescued by Scer\GAL4Act.PU/Mob4UAS.cSa
Mob4EYΔL3 is partially rescued by Scer\GAL4elav-C155/Mob4UAS.cSa
Mob4EYΔL3 is not rescued by Scer\GAL4Mhc.PW/Mob4UAS.cSa
The larval lethality of Mob4EYΔL3 is rescued by the expression of either Mob4Scer\UAS.N.T:Disc\RFP-mCherry or Mob4Scer\UAS.C.T:Disc\RFP-mCherry under the control of either Scer\GAL4Act.PU or Scer\GAL4tub.PU.
Expression of Mob4Scer\UAS.cSa under the control of Scer\GAL4Act.PU rescues the larval lethality of Mob4EYΔL3 homozygotes; the rescued animals being able to pupate and eclose as adults.
Expression of Mob4Scer\UAS.cSa under the control of Scer\GAL4elav-C155 partially rescues the larval lethality of Mob4EYΔL3 homozygotes; the rescued animals are able to pupate but do not eclose as adults.
Expression of Mob4Scer\UAS.cSa under the control of Scer\GAL4Mhc.PW fails to rescue the larval lethality of Mob4EYΔL3 homozygotes.
Expression of Mob4Scer\UAS.cSa under the control of Scer\GAL4elav-C155 partially rescues the supernumerary bouton phenotype observed at the neuromuscular junction in Mob4EYΔL3 mutant larvae.
Expression of Mob4Scer\UAS.cSa under the control of Scer\GAL4elav-C155 rescues the EPSC phenotype of seen at the neuromuscular junction in Mob4EYΔL3 larvae, but expression of Mob4Scer\UAS.cSa under the control of Scer\GAL4Mhc.PW does not rescue this phenotype.
