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General Information
Symbol
Hsap\MAPTE14.UAS
Species
H. sapiens
Name
FlyBase ID
FBal0246848
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-TauE14, tauE14, UAS-hTauE14
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of Hsap\MAPT, carrying mutations in fourteen disease-associated Ser-Thr-Pro sites (Thr 111, Thr 153, Ser 175, Thr 181, Ser 199, Ser 202, Thr 205, Thr 212, Thr 217, Thr 231, Ser 235, Ser 396, Ser 404, and Ser 422) to glutamate.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

4 week old flies expressing Hsap\MAPTE14.Scer\UAS under the control of Scer\GAL4ple.PF show a significant loss of PPL1 dopaminergic neurons in the brain. No significant loss of these neurons is seen in younger flies.

The mitochondria in the neurons of adult brains expressing Hsap\MAPTE14.Scer\UAS under the control of Scer\GAL4elav.PU are markedly elongated. Mitochondria length is on average greater than twice that of controls.

Overexpression of Hsap\MAPTE14.UAS under the control of Scer\GAL4RapGAP1-OK6 in larval segmental nerves effects synaptic vesicle kinetics (there is a significant increase in instant velocity); there is no significant reduction in MT density; no effect on the number of mobile vesicles within axons. Emerging flies have normal wing expansion.

Compared to controls, expression of Hsap\MAPTE14.Scer\UAS under the control of Scer\GAL4elav.PU results in severe mushroom body (MB) abnormalities. However, although the number of MB neurons is severely reduced, they are not completely eliminated.

Expression of Hsap\MAPTE14.Scer\UAS in the developing eye under the control of Scer\GAL4GMR.PF generates a severe rough eye phenotype. The orderly ommatidial architecture is disorganized and photoreceptors are fused with missing mechanosensory bristles.

Expression of Hsap\MAPTE14.Scer\UAS in the developing external sensory organs under the control of Scer\GAL4Eq1 generates a moderate degree of bristle loss.

Expression of Hsap\MAPTE14.Scer\UAS under the control of Scer\GAL4GMR.PF results in a severe rough eye phenotype.

Expression of Hsap\MAPTE14.Scer\UAS under the control of Scer\GAL4elav.PU results in a rough eye phenotype.

Conditional expression of Hsap\MAPTE14.Scer\UAS only during the adult stages under the control of Scer\GAL4elav.PU and Scer\GAL80ts.αTub84B substantially and significantly elongates the lifespan of flies compared with wild-type.

Expression of Hsap\MAPTE14.Scer\UAS under the control of Scer\GAL4elav.PU results in neurodegeneration of both the cortex and neuropil in 5-day old flies, with these regions exhibiting an increase in neuron apoptosis, as compared to controls.

Expression of Hsap\MAPTE14.Scer\UAS in the retina under the control of Scer\GAL4GMR.PF results in a rough-eye phenotype.

Expression of Hsap\MAPTE14.Scer\UAS under the control of Scer\GAL4GMR.PF results in a rough-eye phenotype.

Expression of Hsap\MAPTE14.Scer\UAS in the brain under the control of Scer\GAL4elav.PLu results in neurodegeneration, and increased cell cycle activation is also observed.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Either Trxr-1Δ1 heterozygotes or Sod2n283 heterozygotes expressing Hsap\MAPTE14.Scer\UAS under the control of Scer\GAL4elav.PU are short lived as compared to controls.

Expression of Act5CScer\UAS.T:Avic\GFP in animals expressing Hsap\MAPTE14.Scer\UAS in the retina under the control of Scer\GAL4GMR.PF enhances the rough-eye phenotype.

The rough-eye phenotype observed in animals expressing Hsap\MAPTE14.Scer\UAS in the retina under the control of Scer\GAL4GMR.PF is enhanced in a Act5CG0010/+ background.

Expression of Hsap\APPAβ42.Scer\UAS in flies expressing Hsap\MAPTE14.Scer\UAS under the control of Scer\GAL4GMR.PF does not enhance the rough-eye phenotype.

The rough-eye phenotype observed in animals expressing Hsap\MAPTE14.Scer\UAS under the control of Scer\GAL4GMR.PF is suppressed by co-expression of dapScer\UAS.cdNa and RbfScer\UAS.cDa.

The rough-eye phenotype observed in animals expressing Hsap\MAPTE14.Scer\UAS under the control of Scer\GAL4GMR.PF is enhanced by co-expression of CycDScer\UAS.cMa.

The rough-eye phenotype observed in animals expressing Hsap\MAPTE14.Scer\UAS under the control of Scer\GAL4GMR.PF is enhanced by co-expression of CycEScer\UAS.cUa.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Hsap\MAPTE14.Scer\UAS
Hsap\MAPTE14.UAS
Name Synonyms
Secondary FlyBase IDs
    References (14)