Allele Dmel\kis^LM27

General Information
Symbol	Dmel\kis^LM27	Species	D. melanogaster
Name		FlyBase ID	FBal0246953
Feature type	allele	Associated gene	Dmel\kis

Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Melicharek et al., 2008)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	neuroanatomy defective \| adult stage \| somatic clone (Melicharek et al., 2010) neuroanatomy defective \| pupal stage \| somatic clone (Melicharek et al., 2010) neuroanatomy defective \| somatic clone \| third instar larval stage (Melicharek et al., 2010)
Phenotype Manifest In
	dorsal cluster neuron \| adult stage \| somatic clone (Melicharek et al., 2010) dorsal cluster neuron \| somatic clone \| third instar larval stage (Melicharek et al., 2010) eye photoreceptor cell \| somatic clone \| third instar larval stage (Melicharek et al., 2010) mushroom body alpha-lobe \| adult stage \| somatic clone (Melicharek et al., 2010) mushroom body beta-lobe \| adult stage \| somatic clone (Melicharek et al., 2010) mushroom body gamma-lobe \| adult stage \| somatic clone (Melicharek et al., 2010) mushroom body gamma-lobe \| pupal stage \| somatic clone (Melicharek et al., 2010)
Detailed Description
	Statement Reference Homozygous γ Kenyon neurons undergo proper dendritic pruning during pupal development, but they display defects in their axonal pruning and continue to display unpruned γ axons projecting into the α lobes 40% of the time in pupal brains. Cells in homozygous clones in the α, β and γ lobes of the mushroom body have normal dendrite morphology, but show axonal migration defects with axons crossing the midline (55% penetrance), cell position defects (36% penetrance) and defects in the morphology of the lobe (55% penetrance). Homozygous dorsal cluster neuron clones in the larva show a number of defects; 53% show abnormalities in position within the brain, 32% show defects in axon migration (the neurons fail to migrate to their proper contralateral targets) and 32% of cell bodies are not part of the larger cell body cluster. Homozygous adult dorsal cluster neurons do not show defects in dendrite formation. However, they show a severe reduction in the number of axons extending into the lobula and subsequently extending from the lobula into the medulla. Homozygous clones in the developing third instar larval retina show abnormal axonal migration into the developing brain, with photoreceptor axons extending beyond their normal targets and radiating out into the surrounding brain areas. These axons also show abnormal defasciculation of axonal bundles in the optic stalk. External eye morphology appears normal in adults containing homozygous clones in the retina. (Melicharek et al., 2010)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Statement Reference kis[+]/kis^LM27 is an enhancer of eye \| heat sensitive phenotype of Df(3R)p13/ato¹⁰⁹⁰ (Melicharek et al., 2008) kis[+]/kis^LM27 is an enhancer of photoreceptor cell \| heat sensitive phenotype of Df(3R)p13/ato¹⁰⁹⁰ (Melicharek et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference A kis[LM27] heterozygous background gives a dominant mild enhancement to the ato[1090]/Df(3R)p13 eye phenotype at 25[o]C. (Melicharek et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	kis¹ (Melicharek et al., 2008) kis^k13416 (Melicharek et al., 2008)
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	kis^LM27 (Melicharek et al., 2010, Melicharek et al., 2008)
Name Synonym	kismet^LM27 (Melicharek et al., 2008)
Secondary FlyBase IDs

References ( 2 )
Research paper	Melicharek et al., 2010, Hum. Mol. Genet. 19(21): 4253--4264 Kismet/CHD7 regulates axon morphology, memory and locomotion in a Drosophila model of CHARGE syndrome. [FBrf0212001] Melicharek et al., 2008, Genetics 180(4): 2095--2110 Identification of novel regulators of atonal expression in the developing Drosophila retina. [FBrf0207360]

Allele Dmel\kisLM27

Allele Dmel\kis^LM27