Allele Dmel\Acsl^KO

General Information
Symbol	Dmel\Acsl^KO	Species	D. melanogaster
Name		FlyBase ID	FBal0247206
Feature type	allele	Associated gene	Dmel\Acsl
Also Known As	dAcsl^KO

Allele class	loss of function allele
Mutagen	FLPase
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	loss of function allele (Zhang et al., 2011)
Mutagen	FLPase (Zhang and Wang, 2010.11.5)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	PBac{RB}Acsl^e02676 (Zhang and Wang, 2010.11.5) PBac{WH}f02764 (Zhang and Wang, 2010.11.5)
Nature of the lesion	Statement Reference Recombination between the two progenitor insertions has resulted in the deletion of the sequence between them. (Zhang and Wang, 2010.11.5)
Caused by aberration	Df(2R)Acsl^KO (Zhang and Wang, 2010.11.5)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| first instar larval stage \| recessive (Liu et al., 2011) neuroanatomy defective \| larval stage (with Acsl⁰⁵⁸⁴⁷) (Liu et al., 2011) neurophysiology defective (with Acsl⁰⁵⁸⁴⁷) (Liu et al., 2011)
Phenotype Manifest In
	bouton (with Acsl⁰⁵⁸⁴⁷) (Liu et al., 2011) embryonic/larval neuromuscular junction (with Acsl⁰⁵⁸⁴⁷) (Liu et al., 2011) larval abdominal segment 6 (with Acsl⁰⁵⁸⁴⁷) (Liu et al., 2011) larval abdominal segment 7 (with Acsl⁰⁵⁸⁴⁷) (Liu et al., 2011) segmental nerve (with Acsl⁰⁵⁸⁴⁷) (Liu et al., 2011) synapse (with Acsl⁰⁵⁸⁴⁷) (Liu et al., 2011)
Detailed Description
	Statement Reference Acsl[KO] is an early larval lethal allele. In the cross section of the larval segmental nerves of Acsl[KO]/Acsl[05847] mutants, there are conspicuous axonal swellings packed with heterogeneous membranous organelles. These aggregates, most often observed in posterior axons, include dark, prelysosomal and multivesicular bodies. In some mutant larvae, autophagosomes are also observed where a cluster of large clear-core vesicles are surrounded by a double-layer membrane. The mutant nerves also display lamellated bodies which resemble autolysosomes. In other cases, clusters of large clear-core vesicles are observed. These membrane aggregates are rarely found in wild-type axons. Acsl[KO]/Acsl[05847] mutants display pronounced dystrophy of larval neuromuscular junction 4 (NMJ4) in the posterior abdominal segments A6 and A7, while the muscle and larval size are comparable to wild-type. The bouton number and the synaptic area are significantly reduced in Acsl[KO]/Acsl[05847] mutants compared with wild-type. The synaptic area of Acsl[KO]/Acsl[05847] in Acsl[KO]/Acsl[05847] mutants is comparable to that of wild-types 2 days after egg laying (AEL). Synapse growth during 2-3.5 days AEL of Acsl[KO]/Acsl[05847] mutants is markedly slower than that in wild-types. From 3.5 to 5 days AEL, the mutant NMJ synapses apparently retract. The evoked excitatory junction potentials (EJPs) are significantly reduced in Acsl[KO]/Acsl[05847] mutant larvae recorded from muscle 6 in the posterior abdominal segment. Miniature EJPs (mEJPs, also known as quantal size) are normal in Acsl[KO]/Acsl[05847] mutants. Quantal content is significantly reduced in Acsl[KO]/Acsl[05847] mutants. (Liu et al., 2011)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Acsl⁰⁵⁸⁴⁷/Acsl^KO has neuroanatomy defective \| larval stage phenotype, suppressible by Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}/Scer\GAL4^αTub84B.PL (Liu et al., 2011) Acsl⁰⁵⁸⁴⁷/Acsl^KO has neurophysiology defective phenotype, suppressible \| partially by Scer\GAL4^Mhc.PW/Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} (Liu et al., 2011) Acsl⁰⁵⁸⁴⁷/Acsl^KO has neurophysiology defective phenotype, suppressible by Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC}/Scer\GAL4^αTub84B.PL (Liu et al., 2011) Acsl⁰⁵⁸⁴⁷/Acsl^KO has neurophysiology defective phenotype, suppressible by Scer\GAL4^elav.PU/Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} (Liu et al., 2011)
Phenotype Manifest In
Suppressed by
Statement Reference Acsl⁰⁵⁸⁴⁷/Acsl^KO has bouton phenotype, suppressible by Scer\GAL4^elav.PU/Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} (Liu et al., 2011) Acsl⁰⁵⁸⁴⁷/Acsl^KO has synapse phenotype, suppressible by Scer\GAL4^{elav.Switch.PO}/Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} (Liu et al., 2011)
NOT suppressed by
Statement Reference Acsl⁰⁵⁸⁴⁷/Acsl^KO has bouton phenotype, non-suppressible by Scer\GAL4^Mhc.PW/Hsap\ACSL4^{Scer\UAS.L.T:Hsap\MYC} (Liu et al., 2011)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] in postsynaptic muscles driven by Scer\GAL4[Mhc.PW] does not suppress the dystrophic neuromuscular junctions of Acsl[KO]/Acsl[05847] mutants for bouton number. Neuronal expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] at 2.5 days after egg laying (AEL) fully suppresses the reduced synaptic area phenotype of Acsl[KO]/Acsl[05847] mutants. Neuronal expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] driven by Scer\GAL4[elav.PU] restores the bouton number to wild-type levels in Acsl[KO]/Acsl[05847] mutants. The neuromuscular junction phenotypes of Acsl[KO]/Acsl[05847] mutants can be fully suppressed by ubiquitous expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] under the control of Scer\GAL4[αTub84B.PL]. The reduced EJPs and quantal content are fully rescued by Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] expressed in either ubiquitously via Scer\GAL4[αTub84B.PL] or pan-neuronally via Scer\GAL4[elav.PU]. EJPs in animals with Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] expressed pan-neuronally are mildly but significantly higher than that in wild-types. Postsynaptic expression of Hsap\ACSL4[Scer\UAS.L.T:Hsap\MYC] driven by Scer\GAL4[Mhc.PW] in Acsl[KO]/Acsl[05847] mutant background slightly suppressed the EJP phenotype. (Liu et al., 2011)
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	32331 y¹ w^*; Df(2R)Acsl^KO, PBac{RB3.WH3}Acsl^KO Acsl^KO/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7; +/TM6B, Tb¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Acsl^KO (Zhang and Wang, 2010.11.5) dAcsl^KO (Liu et al., 2011, Zhang et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 3 )
Research paper	Liu et al., 2011, J. Neurosci. 31(6): 2052--2063 Drosophila Acyl-CoA Synthetase Long-Chain Family Member 4 Regulates Axonal Transport of Synaptic Vesicles and Is Required for Synaptic Development and Transmission. [FBrf0212968] Zhang et al., 2011, Dev. Biol. 353(2): 259--265 Drosophila long-chain acyl-CoA synthetase acts like a gap gene in embryonic segmentation. [FBrf0213522]
Personal communication to FlyBase	Zhang and Wang, 2010.11.5, Acsl Df from Yi Zhang and Zhaohui Wang. Acsl Df from Yi Zhang and Zhaohui Wang. [FBrf0212314]

Allele Dmel\AcslKO

Allele Dmel\Acsl^KO