Allele Zzzz\lef^Scer\UAS.cGa

General Information
Symbol	Zzzz\lef^Scer\UAS.cGa	Species	a. artificial
Name		FlyBase ID	FBal0247339
Feature type	allele	Associated gene	Zzzz\lef

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Guichard et al., 2006)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Zzzz\lef coding sequences where the bacterial signal sequences, comprising the first 33 amino acid residues have been removed and are replaced with a methionine preceded by an optimal Drosophila Kozak's sequence (GCCAGC). (Guichard et al., 2006)
Carried in construct	P{UAS-Zzzz\lef.G} (Guichard et al., 2006, Guichard et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	lethal, with Scer\GAL4^Act.PU (Guichard et al., 2006) lethal, with Scer\GAL4^tsh-md621 (Guichard et al., 2006) lethal, with Scer\GAL4^twi.PG (Guichard et al., 2006) partially lethal - majority die, with Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Guichard et al., 2006)
Phenotype Manifest In
	embryo, with Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Guichard et al., 2006) embryo, with Scer\GAL4^pnr-MD237 (Guichard et al., 2006) embryonic/first instar larval cuticle, with Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Guichard et al., 2006) embryonic head, with Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Guichard et al., 2006) embryonic leading edge cell, with Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Guichard et al., 2006) filamentous actin, with Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} (Guichard et al., 2006) wing, with Scer\GAL4^Bx-MS1096 (Guichard et al., 2006) wing vein, with Scer\GAL4^Bx-MS1096 (Guichard et al., 2006)
Detailed Description
	Statement Reference Expression of Zzzz\lef[Scer\UAS.cGa] in the wing disc primordium under the control of Scer\GAL4[bs-1348] produces no obvious phenotype. Expression of Zzzz\lef[Scer\UAS.cGa] in the wing disc primordium under the control of Scer\GAL4[Bx-MS1096] produces no obvious phenotype. (Guichard et al., 2010) Expression of Zzzz\lef[Scer\UAS.cGa] under the control of Scer\GAL4[tsh-md621], Scer\GAL4[twi.PG] or Scer\GAL4[Act.PU] results in almost complete lethality. Expression of Zzzz\lef[Scer\UAS.cGa] under the control of Scer\GAL4[mat.αTub67C.T:Hsim\VP16] results in near complete lethality. Embryos exhibit a range of developmental defects. The least severely affected embryos have a U-shaped phenotype, which is typical of mutations interfering with dorsal closure. More severely affected embryos are greatly reduced in size, have dorsal holes in the cuticle, and lack all head skeletal structures. In addition, these embryos exhibit severe defects in the F-actin network in leading-edge cells, which forms a regular line in wild-type embryos. Expression of Zzzz\lef[Scer\UAS.cGa] under the control of Scer\GAL4[pnr-MD237] results in a dorsal cleft phenotype. Strong expression of Zzzz\lef[Scer\UAS.cGa] in the wing disc under the control of Scer\GAL4[Bx-MS1096] causes small scooped wings, which are elongated along the proximal-distal axis and distal vein truncations. Males with three copies of the Zzzz\lef[Scer\UAS.cGa] transgene, driven by Scer\GAL4[Bx-MS1096] at 29[o]C exhibit the small wing vein phenotype with almost 100% penetrance, approximately 50% of these individuals also exhibit vein truncations. (Guichard et al., 2006)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement Reference Zzzz\lef^Scer\UAS.cGa has wing phenotype, enhanceable by Dsor1^r1/Scer\GAL4^Bx-MS1096 (Guichard et al., 2006) Zzzz\lef^Scer\UAS.cGa has wing vein phenotype, enhanceable by Dsor1^r1/Scer\GAL4^Bx-MS1096 (Guichard et al., 2006)
Suppressor of
Statement Reference Scer\GAL4^CY2/Zzzz\lef^Scer\UAS.cGa is a suppressor of wing vein \| ectopic phenotype of phl^Act.Scer\UAS (Guichard et al., 2006) Scer\GAL4^CY2/Zzzz\lef^Scer\UAS.cGa is a suppressor of wing vein L5 phenotype of hep^CA.Scer\UAS (Guichard et al., 2006)
NOT Suppressor of
Statement Reference Zzzz\lef^Scer\UAS.cGa/Scer\GAL4^Bx-MS1096 is a non-suppressor of wing vein \| ectopic phenotype of rl^Sem.Scer\UAS (Guichard et al., 2006)
Other
Statement Reference Rab11^{S25N.Scer\UAS.P\T.T:Avic\GFP-YFP}, Scer\GAL4^Bx-MS1096, Zzzz\lef^Scer\UAS.cGa has wing margin phenotype (Guichard et al., 2010) Scer\GAL4^bs-1348, Zzzz\EF^Scer\UAS.cGa, Zzzz\lef^Scer\UAS.cGa has wing margin phenotype (Guichard et al., 2010) Scer\GAL4^Bx-MS1096, Zzzz\EF^Scer\UAS.cGa, Zzzz\lef^Scer\UAS.cGa has wing margin phenotype (Guichard et al., 2010) Scer\GAL4^Bx-MS1096, Zzzz\lef^Scer\UAS.cGa, kni^ri-1 has wing vein phenotype (Guichard et al., 2006) Scer\GAL4^Bx-MS1096, Zzzz\lef^Scer\UAS.cGa, rho^ve-1 has wing vein phenotype (Guichard et al., 2006) Scer\GAL4^Bx-MS1096, Zzzz\lef^Scer\UAS.cGa, vn^unspecified has wing vein phenotype (Guichard et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference Co-expression of Zzzz\EF[Scer\UAS.cGa] and Zzzz\lef[Scer\UAS.cGa] in the wing disc primordium under the control of Scer\GAL4[bs-1348] produces a strong and penetrant wing margin notching phenotype. Co-expression of Zzzz\EF[Scer\UAS.cGa] and Zzzz\lef[Scer\UAS.cGa] in the wing disc primordium under the control of Scer\GAL4[Bx-MS1096] produces a strong and penetrant wing margin notching phenotype. Co-expression of Rab11[S25N.Scer\UAS.P\T.T:Avic\GFP-YFP] with Zzzz\lef[Scer\UAS.cGa] in the wing disc primordium produces a strong and penetrant wing margin notching phenotype (when both are under the control of Scer\GAL4[Bx-MS1096]). (Guichard et al., 2010) Moderate expression of Zzzz\lef[Scer\UAS.cGa] under the control of Scer\GAL4[Bx-MS1096] in rho[ve-1] heterozygotes results in wing vein loss, while moderate Zzzz\lef[Scer\UAS.cGa] expression (with Scer\GAL4[Bx-MS1096]) alone doesn't have this phenotype. Moderate expression of Zzzz\lef[Scer\UAS.cGa] under the control of Scer\GAL4[Bx-MS1096] in vn[unspecified] heterozygotes results in wing vein loss, while moderate Zzzz\lef[Scer\UAS.cGa] expression (with Scer\GAL4[Bx-MS1096]) alone doesn't have this phenotype. Moderate expression of Zzzz\lef[Scer\UAS.cGa] under the control of Scer\GAL4[Bx-MS1096] in kni[ri-1] heterozygotes results in wing vein loss, while moderate Zzzz\lef[Scer\UAS.cGa] expression (with Scer\GAL4[Bx-MS1096]) alone doesn't have this phenotype. Expression of Zzzz\lef[Scer\UAS.cGa] does not suppress the ectopic vein phenotype of rl[Sem.Scer\UAS] under the control of Scer\GAL4[Bx-MS1096]. Expression of Zzzz\lef[Scer\UAS.cGa] suppresses the strong ectopic vein phenotype of phl[Act.Scer\UAS] under the control of Scer\GAL4[CY2]. Moderate expression of Zzzz\lef[Scer\UAS.cGa] under the control of Scer\GAL4[Bx-MS1096] in Dsor1[r1] results in an aggravated Zzzz\lef[Scer\UAS.cGa] (Scer\GAL4[Bx-MS1096]) phenotype consisting of more pronounced scooped wings and distal vein truncations. (Guichard et al., 2006)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Zzzz\lef^Scer\UAS.cGa
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Guichard et al., 2010, Nature 467(7317): 854--858 Anthrax toxins cooperatively inhibit endocytic recycling by the Rab11/Sec15 exocyst. [FBrf0212093] Guichard et al., 2006, Proc. Natl. Acad. Sci. U.S.A. 103(9): 3244--3249 Anthrax lethal factor and edema factor act on conserved targets in Drosophila. [FBrf0191441]

Allele Zzzz\lefScer\UAS.cGa

Allele Zzzz\lef^Scer\UAS.cGa