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General Information
Symbol
Mmus\PrnpP101L.UAS.Tag:3F4
Species
M. musculus
Name
FlyBase ID
FBal0247618
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UASt regulatory sequences drive expression of the open reading frame of Mmus\Prnp with a Pro to Leu substitution at residue 101 and tagged with Tag:3F4 at residues 109M-112M..
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Flies expressing Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 (ubiquitously, under the control of Scer\GAL4αTub84B.PL) increases susceptibility to DTT. After exposure to 25mM DTT for 144 hours, more than 70% of control flies are alive, whereas less than 50% of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 flies survive. These flies also show significantly increased resistance to H[[2]]O[[2]] compared to controls. After treatment with 1% H[[2]]O[[2]], more than 70% of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 flies survive, while less than 60% of control flies remain. Conversely, sensitivity to paraquat is significantly increased in Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 flies. After 36 hours of paraquat exposure, less than 30% of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 flies are alive, while more than 60% of control flies survive. After 60 hours of exposure to 20mM 3-MA, the number of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4-expressing (under the control of Scer\GAL4αTub84B.PL) flies alive is significantly reduced compared to control flies. However, 40υM wortmannin treatment does not induce any significant difference in the number of live flies between Scer\GAL4αTub84B.PL/Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 and control flies. Scer\GAL4αTub84B.PL/Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 flies treated with 40υM LY294002 do not show any difference from control flies. Scer\GAL4αTub84B.PL/Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 flies show a significantly increased susceptibility to LiCl but not rapamycin. Scer\GAL4αTub84B.PL/Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 flies also show an increased susceptibility to the simultaneous treatment of rapamycin and LiCl that is similar to that of LiCl alone.
Expression of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 driven by Scer\GAL4αTub84B.PL induces severe defects in climbing ability and early death. Type 1 glutamatergic larval neuromusclular junctions expressing Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 driven by Scer\GAL4αTub84B.PL have more small-sized satellite boutons than those of controls. Expression of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 driven by Scer\GAL4Act5C.PU induces severe defects in climbing ability and early death. When Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 is expressed via by Scer\GAL4ChAT.7.4, more than 90% of flies fail the climbing test at 20 days of age. When Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 is expressed via by Scer\GAL4elav-C155, more than 90% of flies fail the climbing test at 55 days of age. Flies expressing Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 driven by one of the drivers Scer\GAL4Ddc.PL or Scer\GAL4Tab2-201Y or Scer\GAL4Gli.PS or Scer\GAL4C57 do not lead to any significant climbing defects, as compared to controls.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Expression of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 significantly enhances the pathogenic eye defects seen upon expression of Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 in the eye (both lines under the control of Scer\GAL4GMR.PF), with approximately 53% of ommatidia displaying aberrant structures. Expression of Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 significantly enhances the pathogenic eye defects seen upon expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 in the eye (both lines under the control of Scer\GAL4GMR.PF), with approximately 92% of ommatidia displaying aberrant structures. Expression of Hsap\MJDtr.Q27.Scer\UAS.T:Ivir\HA1 and Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 in the developing eye under the control of Scer\GAL4GMR.PF does not result in any significant structural defects in the eye. Expression of Zzzz\CAG20Q.Scer\UAS.T:Ivir\HA1 and Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 in the developing eye under the control of Scer\GAL4GMR.PF does not result in any significant structural defects in the eye.
Co-expression of BacA\p35Scer\UAS.cUa with Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 driven by Scer\GAL4Cha.7.4 does not suppress the Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4-associated climbing defects.
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4
Mmus\PrnpP101L.UAS.Tag:3F4
Name Synonyms
Secondary FlyBase IDs
    References (2)