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General Information
Symbol
BacA\p35UAS.cUa
Species
N. Autographa californica nucleopolyhedrovirus
Name
Saccharomyces cerevisiae UAS construct a of Unknown
FlyBase ID
FBal0247620
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-p35, UAS::P35
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS regulatory sequences drive expression of BacA\p35.
Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
The expression of BacA\p35Scer\UAS.cUa under the control of either Scer\GAL4Hsp83.PA or Scer\GAL4nos.PU does not lead to a significant proportion of hyperplastic testes, as compared to controls.
Third instar larvae expressing BacA\p35Scer\UAS.cUa under the control of Scer\GAL4477 show no defects in the field coverage and branching of the dendritic arbor in class IV dendritic arborizing neurons compared to controls.
Expression of BacA\p35Scer\UAS.cUa under the control of Scer\GAL4bun-GSG5961 and Scer\GAL4GSG5966 (along with RU486 to induce expression via the GeneSwitch system) does not affect the growth of wild type clones in the adult posterior midgut.
Expression of BacA\p35Scer\UAS.cUa under the control of Scer\GAL4esg-NP5130 (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) does not significantly change the number of mitotic cells in the midgut.
Expression of BacA\p35Scer\UAS.cUa under the control of Scer\GAL4e22c does not alter the number of nuclei in the central syncytium that forms around a puncture wound in the epidermis of third instar larvae, nor does it induce syncytia formation in unwounded epithelium (either with the Scer\GAL4e22c driver or with Scer\GAL4pnr-MD237 and tub-Gal80[ts] to limit the time of expression to third instar).
Transplantation of wing disc tissue expressing BacA\p35Scer\UAS.cUa under the control of Scer\GAL4ap.PU into the abdomen of adult females (and maintained for 12 days) does not result in tissue growth.
Under control conditions, the expression of BacA\p35UAS.cUa under the control of Scer\GAL4ppk.PG does not significantly affect the number of sensory axons per nerve in third instar larvae, as compared to controls.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
BacA\p35UAS.cUa/Scer\GAL4so.PU is a non-suppressor of eye phenotype of gl60j
BacA\p35UAS.cUa/Scer\GAL4hs.PU is a non-suppressor of eye phenotype of gl60j
Other
Additional Comments
Genetic Interactions
Statement
Reference
The dendritic field coverage defects and loss of terminal branches observed in class IV dendritic arborizing neurons of third instar larvae expressing nosdsRNA.Scer\UAS.WIZ under the control of Scer\GAL4477 is suppressed by co-expression of BacA\p35Scer\UAS.cUa.
72.5% of flies expressing DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8 under the control of Scer\GAL4ey.PU have eye tumours, with 4.9% having macroscopically visible secondary tumour growths derived from the developing retina. The incidence of primary and secondary tumours in these "eyeful" flies is slightly enhanced by co-expression of ctdsRNA.cGa.Scer\UAS, but is greatly enhanced if the "eyeful" flies also co-express both ctdsRNA.cGa.Scer\UAS and BacA\p35Scer\UAS.cUa. Flies co-expressing ctdsRNA.cGa.Scer\UAS and DlScer\UAS.cDa under the control of Scer\GAL4ey.PU show a low frequency of primary eye tumours and secondary tumours. The incidence of primary and secondary tumours is enhanced by co-expression of BacA\p35Scer\UAS.cUa. Flies co-expressing vvlJF02126, DlScer\UAS.cDa and BacA\p35Scer\UAS.cUa under the control BacA\p35Scer\UAS.cUaof Scer\GAL4ey.PU show a low frequency of primary eye tumours and of secondary tumours.
Xenogenetic Interactions
Statement
Reference
The salivary gland tissue persistence phenotype observed in hrmΔ1/Df(2R)BSC696 transheterozygous pupae is enhanced by the expression of BacA\p35UAS.cUa under the control of Scer\GAL4fkh.PH salivary gland tissue, leading to more intact gland fragments 24h after puparium formation; an enhanced phenotype is also observed in hrmΔ1 heterozygotes expressing BacA\p35UAS.cUa under the control of Scer\GAL4fkh.PH.
Flies co-expressing Hsap\APP2xAPP.SP.Scer\UAS and BacA\p35Scer\UAS.cUa under either Scer\GAL4Toll-6-D42 or Scer\GAL4e22c are lethal and do not reach adulthood.
Co-expression of BacA\p35Scer\UAS.cUa suppresses the reduced size and disruption of adult eyes in flies with DCAF12Scer\UAS.cHa driven by Scer\GAL4GMR.PU. BacA\p35Scer\UAS.cUa expression suppresses the apoptotic phenotype (of limited clonal growth) in picPL12c/picPL12c eye clones.
The co-expression of BacA\p35UAS.cUa enhances the cell dispersion within the ptc-expressing domain of the third instar larval wing disc induced by the expression of tsrdsRNA.UAS under the control of Scer\GAL4ptc.PU, even leading to the enlargement of the ptc domain and to wrinkles in the disc. The co-expression of tsrdsRNA.UAS and BacA\p35UAS.cUa under the control of Scer\GAL4ptc.PU leads to a significant increase in mitotic index (phospho-H3 immunofluorescence) in the ptc-expressing domain within the third instar larval wing disc, as compared to controls.
Expression of BacA\p35Scer\UAS.cUa under the control of Scer\GAL4Act.PU suppresses the apoptosis phenotype, but fails to rescue the delayed photoreceptor differentiation phenotype of Ubr3B/Ubr3B mutant clones.
Expression of BacA\p35Scer\UAS.cUa under the control of Scer\GAL4so.PU or Scer\GAL4hs.PU results in a decrease in cell death at 48 h APF, but did not rescue the eye morphology, retina structure, or photoreceptor differentiation defects of gl60j/gl60j mutants.
Co-expression of BacA\p35Scer\UAS.cUa partially rescues the wing atrophy phenotype of flies expressing Lsd-1GD6641 under the control of Scer\GAL4Bx-MS1096.
Co-expression of BacA\p35Scer\UAS.cUa suppresses the reduction of eye pigments and weakly suppresses the abnormal formation of ommatidia and bristles and reduced eye size seen in Scer\GAL4GMR.PS>Hsap\BCL2L13Scer\UAS.cNa flies. Co-expression of BacA\p35Scer\UAS.cUa in Scer\GAL4GMR.PS>Hsap\BCL2L13ΔTM.Scer\UAS flies does not result in eye phenotypes. Co-expression of BacA\p35Scer\UAS.cUa partially suppresses aberrant morphology of cone and pigment cells in the Scer\GAL4GMR.PS>Hsap\BCL2L13Scer\UAS.cNa pupal retina 42hr after puparium formation.
The small clone size as well as the lack of differentiated enterocytes and enteroendocrine cells in GATAe1 homozygote MARCM clone in adult posterior midgut is not rescued by expression of BacA\p35Scer\UAS.cUa under the control of Scer\GAL4Act5C.PI. The loss of esg-positive cells as well as their aberrant morphology in the adult posterior midgut expressing GATAeGD4152 under the control of Scer\GAL4NP6267 (using tub-Gal80[ts] to limit the time of expression) cannot be rescued by the combined co-expression of both Diap1Scer\UAS.T:Hsap\MYC and BacA\p35Scer\UAS.cUa.
Co-expression of both aPKCdsRNA.cRa.Scer\UAS and BacA\p35Scer\UAS.cUa under the control of Scer\GAL4ap.PU results in tissue overgrowth and loss of apicobasal polarity in the wing disc.
Expression of BacA\p35Scer\UAS.cUa driven by Scer\GAL4tub.PU does not significantly increase the number of cells per clone in Dis32/Dis32 second instar larval wing discs.
Expression of BacA\p35Scer\UAS.cUa under the control of Scer\GAL4bun-GSG5961 and Scer\GAL4GSG5966 (along with RU486 to induce expression via the GeneSwitch system) in the gut fully restores growth of wild type clones in the presence of ApcQ8/ApcQ8, Apc2g10/Apc2g10 mutant clones in the posterior midgut, and suppresses the growth of ApcQ8/ApcQ8, Apc2g10/Apc2g10 mutant clones; however, ApcQ8/ApcQ8, Apc2g10/Apc2g10 mutant clones expressing BacA\p35Scer\UAS.cUa under the control of Scer\GAL4tub.PU do not have suppressed growth.
Co-expression of BacA\p35Scer\UAS.cUa rescues heart structural defects seen in Scer\GAL4tin.CΔ4>ScoxGD898 flies.
Co-expression of BacA\p35Scer\UAS.cUa does not rescue the decreased cell proliferation observed in the midguts of flies expressing bunGD1392 or MadmGD7155 under the control of Scer\GAL4esg-NP5130.
Expression of BacA\p35Scer\UAS.cUa driven by Scer\GAL4en.PU in wing discs suppresses increased cell death seen in Ipk220B/Ipk220B mutants. Ipk220B/Ipk220B suppresses the enlarged eyes seen in pharate adults with expression of upd1Scer\UAS.cZa driven by Scer\GAL4GMR.PU, even with co-expression of BacA\p35Scer\UAS.cUa.
Co-expression of BacA\p35Scer\UAS.cUa suppresses the increased cell death induced by expression of Hsap\APLP1Scer\UAS.cMa driven along the anterior/posterior compartment boundary in third instar larval wing discs by Scer\GAL4ptc-559.1.
The formation of abnormal large syncytia in larval epidermis characteristic for third instar larvae expressing stckNIG.7954R under the control of Scer\GAL4e22c is not suppressed by co-expression of any BacA\p35Scer\UAS.cUa. The formation of giant syncytia in the patches of dorsal larval epidermis expressing hepCA.Scer\UAS under the control of Scer\GAL4pnr-MD237 (using tub-Gal80ts to limit the expression to 16 hr-long pulse during the third instar) is not suppressed by co-expression of BacA\p35Scer\UAS.cUa, the number of nuclei within the syncytia is also not changed.
Expression of cmetNIG.6392R and BacA\p35Scer\UAS.cUa in the dorsal compartment of the wing disc, under the control of Scer\GAL4ap.PU, results in a high percentage of cells with DNA content higher than 4n (up to 30%) when compared to control cells. This is accompanied by some tissue overgrowth. The resulting wing primordia are massively overgrown, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35Scer\UAS.cUa-expressing control larvae and ultimately die. Expression of cmetGD11911 and BacA\p35Scer\UAS.cUa in the dorsal compartment of the wing disc, under the control of Scer\GAL4ap.PU, results in a high percentage of cells with DNA content higher than 4n (up to 30%) when compared to control cells. This is accompanied by some tissue overgrowth, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35Scer\UAS.cUa-expressing control larvae and ultimately die. Expression of cmetHMJ21427 and BacA\p35Scer\UAS.cUa in the dorsal compartment of the wing disc, under the control of Scer\GAL4ap.PU, results in a high percentage of cells with DNA content higher than 4n (up to 30%) when compared to control cells. This is accompanied by some tissue overgrowth, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35Scer\UAS.cUa-expressing control larvae and ultimately die. Expression of Kmn1KK111826 and BacA\p35Scer\UAS.cUa in the dorsal compartment of the wing disc, under the control of Scer\GAL4ap.PU, results in a high percentage of cells with DNA content higher than 4n (up to 30%) when compared to control cells. This is accompanied by some tissue overgrowth, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35Scer\UAS.cUa-expressing control larvae and ultimately die. Expression of Kmn1GD9062 and BacA\p35Scer\UAS.cUa in the dorsal compartment of the wing disc, under the control of Scer\GAL4ap.PU, results in a high percentage of cells with DNA content higher than 4n (up to 30%) when compared to control cells. This is accompanied by some tissue overgrowth, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35Scer\UAS.cUa-expressing control larvae and ultimately die. Expression of cmetGD11911 and BacA\p35Scer\UAS.cUa in the posterior compartment of the wing disc, under the control of Scer\GAL4en.PU, results in some tissue overgrowth. The resulting wing primordia are massively overgrown, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35Scer\UAS.cUa-expressing control larvae and ultimately die. Expression of cmetHMJ21427 and BacA\p35Scer\UAS.cUa in the posterior compartment of the wing disc, under the control of Scer\GAL4en.PU, results in some tissue overgrowth. The resulting wing primordia are massively overgrown, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35Scer\UAS.cUa-expressing control larvae and ultimately die. Expression of cmetNIG.6392R and BacA\p35Scer\UAS.cUa in the posterior compartment of the wing disc, under the control of Scer\GAL4en.PU, results in some tissue overgrowth. The resulting wing primordia are massively overgrown, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35Scer\UAS.cUa-expressing control larvae and ultimately die. Expression of Kmn1KK111826 and BacA\p35Scer\UAS.cUa in the posterior compartment of the wing disc, under the control of Scer\GAL4en.PU, results in some tissue overgrowth. The resulting wing primordia are massively overgrown, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35Scer\UAS.cUa-expressing control larvae and ultimately die. Expression of Kmn1GD9062 and BacA\p35Scer\UAS.cUa in the posterior compartment of the wing disc, under the control of Scer\GAL4en.PU, results in some tissue overgrowth. The resulting wing primordia are massively overgrown, and the cell population subjected to genetically induced CIN invade the neighboring wild-type territory. Larvae continue growing for longer than BacA\p35Scer\UAS.cUa-expressing control larvae and ultimately die. Wing disc tissue expressing cmetGD11911 and BacA\p35Scer\UAS.cUa under the control of Scer\GAL4ap.PU that has been transplanted into the abdomen of adult female flies (and maintained there for 12 days) grows several times larger than controls expressing BacA\p35Scer\UAS.cUa alone and exhibits disorganised tissue architecture with extensive folding. Wing disc tissue expressing Kmn1KK111826 and BacA\p35Scer\UAS.cUa under the control of Scer\GAL4ap.PU that has been transplanted into the abdomen of adult female flies (and maintained there for 12 days) grows several times larger than controls expressing BacA\p35Scer\UAS.cUa alone and exhibits disorganised tissue architecture with extensive folding.
Coexpression of BacA\p35UAS.cUa and p53UAS.DΔNp53 under the control of Scer\GAL4nub.PU results in tissue overgrowths in the wing disc; this phenotype is reduced by the additional expression of NTRiP.cUa.
Expression of BacA\p35Scer\UAS.cUa under the control of Scer\GAL4alrm.PD in htlAB42 embryos does not rescue infiltration of astrocyte processes into the neuropil or migration of the ventral-most astrocyte.
Expression of BacA\p35Scer\UAS.cUa under the control of Scer\GAL4sage.PV rescues the salivary gland cell death seen in sageko embryos. The rescued salivary glands have lumen irregularities.
Co-expression of mrnGD4089 and BacA\p35Scer\UAS.cUa under the control of Scer\GAL4Bx-MS1096 results in wings containing a large blister. Co-expression of BacA\p35Scer\UAS.cUa suppresses the increased number of apoptotic cells seen in the wing discs of animals expressing mrnGD4089 under the control of Scer\GAL4Bx-MS1096. The massive apoptosis seen in wing discs co-expressing p53Ct.GUS and mrnGD4089 under the control of Scer\GAL4Bx-MS1096 is suppressed by co-expression of BacA\p35Scer\UAS.cUa.
Inhibition of apoptosis, through co-expression of BacA\p35Scer\UAS.cUa does not affect the Mmus\Gria1Lc.Scer\UAS eye defect (when all are expressed under the control of Scer\GAL4hs.2sev.
Co-expression of BacA\p35Scer\UAS.cUa suppresses the cell death seen in the lymph glands of larvae expressing WScer\UAS.cUa under the control of Scer\GAL4Hml.Δ. Co-expression of BacA\p35Scer\UAS.cUa does not suppress the cell death seen in the lymph glands of larvae expressing NijAScer\UAS.cBa under the control of Scer\GAL4Hml.Δ.
Co-expression of BacA\p35Scer\UAS.cUa using Scer\GAL4bbg-C96 completely suppresses the wing notching phenotype caused by the expression of Zzzz\aopPScer\UAS.T:Hsap\MYC.
Co-expression of BacA\p35Scer\UAS.cUa mildly exacerbates the eye defects caused by co-expression of ZnT63CVDRC.cUa and Zip42C.1Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4GMR.PFa. Co-expression of BacA\p35Scer\UAS.cUa exacerbates the thoracic midline defects caused by co-expression of ZnT63CVDRC.cUa and Zip42C.1Scer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4pnr-MD237.
Co-expression of BacA\p35Scer\UAS.cUa fails to rescue the reduction in size of the posterior compartment of the wing which is caused by expression of PRAS40Scer\UAS.cCa under the control of Scer\GAL4en.PU.
Expression of BacA\p35Scer\UAS.cUa under the control of Scer\GAL4Act.PU rescues the reduction in cell number but not the axon mistargeting phenotypes seen in psidin1 Or59c olfactory receptor neuron (ORN) clones. Expression of BacA\p35Scer\UAS.cUa under the control of Scer\GAL4Act.PU does not rescue the axon mistargeting phenotypes seen in psidinIG978 Or59c ORN clones.
Co-expression of BacA\p35Scer\UAS.cUa suppresses the induction of apoptosis in the eye disc caused by co-expression of E2fScer\UAS.cNa and DpScer\UAS.cDa under the control of Scer\GAL4GMR.PF.
Expression of BacA\p35Scer\UAS.cUa suppresses the rough eye phenotype seen when Zzzz\aopPScer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4GMR.PF.
Complementation and Rescue Data
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Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
BacA\p35Scer\UAS.cUa
BacA\p35UAS.cUa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Unknown
Secondary FlyBase IDs
    References (67)