|Name||Saccharomyces cerevisiae UAS construct a of Sanders||FlyBase ID||FBal0247664|
|Feature type||allele||Associated gene||Hsap\GALE|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
|Carried in construct|
|Phenotype Manifest In|
Hsap\GALEScer\UAS.cSa, Scer\GAL4Act5C.PI, Scer\GAL80ts.αTub84B is a suppressor | heat sensitive of lethal | heat sensitive phenotype of GaleNIG.12030R, Scer\GAL4Act5C.PI, Scer\GAL80ts.αTub84B
Hsap\GALEScer\UAS.cSa, Scer\GAL4Act5C.PI is a suppressor of lethal phenotype of GaleNIG.12030R, Scer\GAL4Act5C.PI
Hsap\GALEScer\UAS.cSa, Scer\GAL4Act5C.PI is a suppressor of short lived | chemical conditional phenotype of GaleNIG.12030R, Scer\GAL4Act5C.PI
|NOT Suppressor of|
|Phenotype Manifest In|
Expression of Hsap\GALE[Scer\UAS.cSa] under the control of Scer\GAL4[Act5C.PI] rescues the lethality caused by expression of Gale[NIG.12030R] under the control of Scer\GAL4[Act5C.PI]. The rescued males and females show normal fecundity (number of offspring produced when mated to wild-type flies of the opposite sex). The short lived phenotype seen in adult males and females expressing Gale[NIG.12030R] under the control of Scer\GAL4[Act5C.PI] from the late larval or early-to-mid-pupal stage onwards (the temperature sensitive Scer\GAL80[ts.αTub84B] allele is used to regulate the timing of expression) in the presence of 175mM galactose is completely suppressed by expression of Hsap\GALE[Scer\UAS.cSa] under the control of Scer\GAL4[Act5C.PI].
Expression of Hsap\GALE[Scer\UAS.cSa] using either Scer\GAL4[arm.PS] or Scer\GAL4[Act5C.PI] rescues the lethality of Gale[Δy]/Gale[f00624] animals. Rescued animals are viable, fertile, and appear morphologically normal. Expression of Hsap\GALE[Scer\UAS.cSa] using Scer\GAL4[Act5C.PI] rescues the lethality of Gale[NIG.12030R], Scer\GAL4[Act5C.PI] animals. Rescued animals are viable, fertile, and appear morphologically normal. Expression of Hsap\GALE[Scer\UAS.cSa] using Scer\GAL4[drm.7.1] partially rescues the lethality of Gale[Δy]/Gale[f00624] animals, whereas use of Scer\GAL4[elav-C155] or Scer\GAL4[c754] is completely insufficient to rescue or delay lethality of this genotype.
|Complementation & Rescue Data|
|Stocks ( 1 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
Saccharomyces cerevisiae UAS construct a of Sanders
|Secondary FlyBase IDs|
|References ( 2 )|