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General Information
Symbol
Dmel\crbGX24w-
Species
D. melanogaster
Name
FlyBase ID
FBal0247912
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
crbGX24, crbko
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
complex substitution
Comment:

A 9944 bp deletion removing most of the coding exons of crb was created by homologous recombination. A 50bp version of the attP site and a loxP site are present in place of the deleted sequence. The deletion was reported to map to 3R:24 ,304,580..24,314,523 in release 5 coordinates.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Most of the crb coding region has been deleted. A 50bp version of the attP site and a loxP site are present in place of the deleted sequence.

Insertion components
TI{TI}crbGX24w-
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

A majority of embryos expressing crbY10A in a crbGX24w-/crbGX24w- background exhibit lethality due to dorsal closure defects, ranging in severity from open cuticle, dorsal holes, closed cuticles but failure to hatch, or kinked larvae. These embryos also exhibit major defects in germ band retraction; a highly disorganized amnioserosa, with an increase in large processes, an increase in the number of apoptotic cells, and aberrant contractions of amnioserosal cells; and exhibit disorganization of elongation of the dorsal-most epidermal cells, and erratic extension of the filopodia extended by these cells. The amnioserosa in these embryos shows fragmentation of the zonula adherens.

A majority of embryos expressing crbY10A in a crbGX24w-/crb11A22 background exhibit lethality due to dorsal closure defects, ranging in severity from open cuticle, dorsal holes, closed cuticles but failure to hatch, or kinked larvae.

Embryos expressing crb+tfos in a crbGX24w-/crbGX24w- background successfully complete dorsal closure, and exhibit normal elongation and filpodia extension of the dorsal-most epidermal cells.

Embryos expressing crbY10F in a crbGX24w-/crbGX24w- background successfully complete dorsal closure and do not show obvious defects in the amnioserosal cells.

Mutant embryos lack a continuous cuticle and only grains of cuticle can be detected. Defects are seen in the tracheal system, with abnormal branching, discontinuities and lack of a discernible lumen being seen.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Other
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Embryos expressing crb+tfos in a crbGX24w-/crbGX24w-, yrt75/yrt75 background exhibit defects in dorsal closure mainly after germ band retraction, although overall amnioserosa integrity is preserved during dorsal closure and most of the zippering is completed, leaving a hole only at the posterior canthus.

Expression of flwScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4332.3 does not lead to any obvious phenotype in embryos expressing crbY10F in a crbGX24w-/crb11A22 background.

yrt75/yrt75 fails to ameliorate the germ band retraction and amnioserosal integrity defects seen in embryos expressing crbY10A in a crbGX24w-/crbGX24w- background.

Expression of flwScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4332.3 largely suppresses the embryonic lethality and dorsal closure defects in embryos expressing crbY10A in a crbGX24w-/crb11A22 background, although some embryos exhibit irregular zippering at the posterior canthus and bunching of the epidermis.

flw6/flw6 or flw6 hemizygosity enhances the embryonic lethality and dorsal closure defects seen in embryos expressing crbY10A in a crbGX24w-/crb11A22 background.

Arp3EP3640/+, or expression of Rho1N19.Scer\UAS, RokCAT-KG.Scer\UAS or MoeK.T559D.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4332.3 partially suppresses the embryonic lethality and dorsal closure defects seen in embryos expressing crbY10A in a crbGX24w-/crb11A22 background.

Rho11B hemizygosity partially suppresses the embryonic lethality and dorsal closure defects seen in embryos expressing crbY10A in a crbGX24w-/crbGX24w- background.

Arpc1Q25st/+, Rok2/Rok2 or Rok2 hemizygosity, or expression of Rac1N17.Scer\UAS or MoeScer\UAS.T:Hsap\MYC under the control of Scer\GAL4332.3, fails to suppress the embryonic lethality and dorsal closure defects seen in embryos expressing crbY10A in a crbGX24w-/crb11A22 background.

Expression of PakAID.Scer\UAS.T:Zzzz\FLAG or shgScer\UAS.cSa under the control of Scer\GAL4332.3 partially suppresses the embryonic lethality and dorsal closure defects seen in embryos expressing crbY10A in a crbGX24w-/crb11A22 background, resulting in some adult survival.

SCARΔ37/+ partially suppresses the embryonic lethality and dorsal closure defects seen in embryos expressing crbY10A in a crbGX24w-/crbGX24w- background, resulting in some adults surviving, although these adults show defects in abdominal development.

Expression of flwScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4332.3 does not lead to any obvious phenotype in embryos expressing crb+tfos in a crbGX24w-/crb11A22 background.

SCARΔ37/+ or Arp3EP3640/+ does not lead to any dorsal closure defects in embryos expressing crb+tfos in a crbGX24w-/crb11A22 background.

Expression of PakScer\UAS.T:Myr-Src64B under the control of Scer\GAL4332.3 in embryos expressing crb+tfos in a crbGX24w-/crb11A22 background leads to complete embryonic lethality with most embryos exhibiting dorsal closure defects ranging in severity from open cuticle and dorsal holes, to closed cuticles but failure to hatch.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by

crbGX24w- is partially rescued by crb+tfos

crbGX24w- is partially rescued by crbEGFP

crbGX24w- is partially rescued by crbY10F

crbGX24w- is partially rescued by crbY10A

Not rescued by
Comments

Expression of crbScer\UAS.cWa under the control of Scer\GAL4332.3 fails to significantly rescue the lethality and dorsal closure defects in embryos expressing crbY10A in a crbGX24w-/crb11A22 background.

87% of animals homozygous for crbGX24w- and carrying two copies of crb+tfos hatch. The embryonic cuticle defect is completely rescued. The adult flies are fertile and do not show any obvious mutant phenotype.

75% of animals homozygous for crbGX24w- and carrying two copies of crbT:Avic\GFP-EGFP hatch. The adult flies are fertile and do not show any obvious mutant phenotype.

82% of animals homozygous for crbGX24w- and carrying two copies of crbY10F hatch and give rise to viable and fertile adults.

crbGX24w- embryos carrying two copies of crbY10A fail to hatch. Some rescue of the embryo cuticle phenotype is seen, with the embryos forming continuous anterior and ventral cuticle with rather fully developed denticle belts. The amnioserosa shows defects from stage 13 onwards and is completely lost at stage 15. The embryos show impaired germ band retraction, head involution and dorsal closure. The leading edge of the epidermis appear uneven and wiggling during dorsal closure. Segments show variable widths.

crbY10A.ΔERLI fails to rescue the cuticle defects of crbGX24w- embryos.

crbGX24w- embryos carrying two copies of crbΔERLI develop only small patches of continuous cuticle.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (11)