FB2025_01 , released February 20, 2025
Allele: Dmel\Ada2bUASp.EGFP
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General Information
Symbol
Dmel\Ada2bUASp.EGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0247963
Feature type
allele
Associated gene
Dmel\Ada2b
Associated Insertion(s)
Carried in Construct
P{UASp-Ada2b.EGFP}
Key Links
Transgenic product class
wild_type
(Zsindely et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Zsindely et al., 2009)
Mutagen
in vitro construct
(Zsindely et al., 2009)
Progenitor genotype
Carried in construct
P{UASp-Ada2b.EGFP}
Cytology
Description

In addition to producing a EGFP tagged version of the Ada2b L isoform, this construct also produces the native (untagged) Ada2b S isoform, due to the genomic origin of the transgene and alternative splicing of the transcript.

(Pankotai et al., 2013)

UASp regulatory sequences are fused upstream of sequences encoding the Ada2b L isoform, including the Ada2b regulatory region up to position -400. The Ada2b coding sequence is tagged at the C-terminal end with EGFP. The protein can be expressed either under the control of its own regulatory sequences or, if a GAL4 driver is used, under the control of the UASp regulatory sequences.

(Zsindely et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
Ada2b
UASp
Encoded product / tool
Ada2b
Tagged with
EGFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Ada2bUASp.EGFP partially rescues Ada2bd842

      (Pankotai et al., 2013)

      Ada2bUASp.EGFP partially rescues Ada2bd842

      (Zsindely et al., 2009)

      Scer\GAL4Act5C.PU/Ada2bUASp.EGFP partially rescues Ada2bd842

      (Zsindely et al., 2009)
      Comments

      85% of Ada2bd842 mutants carrying a single copy of Ada2bScer\UAS.P\T.T:Avic\GFP-EGFP emerge as adults.

      (Pankotai et al., 2013)

      Ada2bScer\UAS.P\T.T:Avic\GFP-EGFP is able to produce an 80% phenotypic rescue of Ada2bd842 mutants in the absence of a Scer\GAL4 driver. When expression of Ada2bScer\UAS.P\T.T:Avic\GFP-EGFP is driven by Scer\GAL4Act5C.PU the same degree of rescue of Ada2bd842 is seen as in the absence of the driver.

      (Zsindely et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ada2bScer\UAS.P\T.T:Avic\GFP-EGFP
      Ada2bUASp.EGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)