FlyBase Allele Report: Hsap\APP[Aβ40.UAS.Tag:SS(nec)]

General Information

Symbol

Hsap\APP^{Aβ40.UAS.Tag:SS(nec)}

Species

H. sapiens

Name

FlyBase ID

FBal0248068

Feature type

allele

Associated gene

Hsap\APP

Associated Insertion(s)

Carried in Construct

P{UAS-Aβ.1-40}

Also Known As

UAS-Aβ40, Aβ₄₀, Aβ40, Aβ_1-40, Alz40, UAS-Aβ_1-40

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Crowther et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Aβ.1-40}

Cytology

Description

UASt regulatory sequences drive expression of the Tag:SS(nec) secretion signal peptide fused to the Aβ40 peptide.

(Crowther et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\APP

Tagged with

Tag:SS(nec)

signal sequence

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease 1

CEC

(Wacker et al., 2014)

CEA

(López-Arias et al., 2017, Tabuchi et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^shot-OK307

(Lin et al., 2014)

abnormal neurophysiology | adult stage | progressive, with Scer\GAL4^shot-OK307

(Zhao et al., 2010)

Phenotype Manifest In

dorsal longitudinal indirect flight muscle cell, with Scer\GAL4^shot-OK307

(Lin et al., 2014)

tergosternal muscle cell, with Scer\GAL4^shot-OK307

(Lin et al., 2014)

Detailed Description

Statement

Reference

At ages less than 3 weeks, the latency of evoked dorsal longitudinal flight muscle (DLM) and Tergotrochanter muscle (TTM) excitatory junctional potentials (EJPs) is about 0.8ms in flies expressing one copy of Hsap\APP^{1-40.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^OK307 (compared to 1.2ms in controls). At ages over 3 weeks, the latencies of evoked DLM and TTM EJPs in flies expressing one copy of Hsap\APP^{1-40.Scer\UAS.T:SS-nec} are slightly but not significantly increased, suggesting that ageing does not cause a significant slowdown of neurotransmission in wild-type flies and in flies expressing Hsap\APP^{1-40.Scer\UAS.T:SS-nec}.

(Lin et al., 2014)

Expression of Hsap\APP^{1-40.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^elav.PU does not significantly shorten lifespan compared to controls. No climbing defects are observed in these flies.

Flies expressing Hsap\APP^{1-42.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^elav.PU do not show any defects in locomotor rhythm in LD (12 hours light, 12 hours dark). In DD (total darkness) no different from controls is seen at 5, 35 days or 50 days, but a substantial difference in rhythm is seen at 50 days.

(Long et al., 2014)

Adult flies expressing Hsap\APP^{1-40.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^elav-C155 show similar median lifespan as controls and their climbing ability is also normal.

(Costa et al., 2011)

Flies expressing Hsap\APP^{1-40.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^{elav.Switch.PO} from 2 day post-eclosion onwards (expression induced by feeding flies RU486) do not exhibit any effect on lifespan.

(Sofola et al., 2010)

Expression of Hsap\APP^{1-40.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^shot-OK307 does not significantly change either the flight ability or the lifespan of adult flies. It does however slightly hasten the age-related decline in neurotransmission functionality: the success rate of eliciting excitatory junction potentials (EJP) in the dorsal longitudinal muscle (DLM) fibers in response to brain stimulation is lower compared to controls.

(Zhao et al., 2010)

Flies expressing Hsap\APP^{1-40.Scer\UAS.T:nec} under the control of Scer\GAL4^unspecified have a median survival of about 30 days.

(Luheshi et al., 2007)

Flies expressing Hsap\APP^{1-40.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155 have normal eyes.

Expressing one copy of Hsap\APP^{1-40.Scer\UAS.T:nec} has no effect on survival.

(Crowther et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\APP^{1-40.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^elav.PU in a per⁰¹ mutant background does not significantly shorten lifespan compared to controls. Climbing ability is also unaffected.

(Long et al., 2014)

Expression of Fer1HCH^VDRC.cUa in flies expressing Hsap\APP^{1-40.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155 reduces hatching efficiency.

Expression of Fer2LCH^VDRC.cUa in flies expressing Hsap\APP^{1-40.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155 does not reduce hatching efficiency.

(Liu et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Aβ40

(Costa et al., 2011)

Hsap\APP^{1-40.Scer\UAS.T:SS-nec}

Hsap\APP^{1-40.Scer\UAS.T:nec}

Hsap\APP^{Aβ40.Scer\UAS.T:SS-nec}

Hsap\APP^{Aβ40.UAS.Tag:SS(nec)}

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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