FlyBase Allele Report: Hsap\APP[Arctic.UAS.Tag:SS(nec)]

General Information

Symbol

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}

Species

H. sapiens

Name

FlyBase ID

FBal0248069

Feature type

allele

Associated gene

Hsap\APP

Associated Insertion(s)

Carried in Construct

P{UAS-Aβ.Arctic}

Also Known As

UAS-ArcAβ42, UAS-Arctic Aβ42, AlzArc, UAS-Arctic Aβ_1-42, UAS-Arctic Aβ₄₂, UAS-Aβ_arc, [UAS]Aβ_arc, UAS-Aβ₄₂^Arctic

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Crowther et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Aβ.Arctic}

Cytology

Description

UASt regulatory sequences drive expression of the Tag:SS(nec) secretion signal peptide fused to an Aβ42 peptide carrying the Arctic mutation (amino acid replacement E22G).

(Crowther et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\APP

Tagged with

Tag:SS(nec)

signal sequence

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Alzheimer's disease 1

CEA

(Elovsson et al., 2024, Twyning et al., 2024, Kaldun et al., 2021, Niccoli et al., 2021, Scholes et al., 2020, Briffa et al., 2017, López-Arias et al., 2017, Zhang et al., 2017, Niccoli et al., 2016, Sandin et al., 2016, Han et al., 2015, Tabuchi et al., 2015, Sofola-Adesakin et al., 2014)

CEC

(Catterson et al., 2023, Gandini et al., 2022, Hewitt et al., 2022)

model of Alzheimer's disease

CEA

(Long et al., 2014, Huang et al., 2013, Rogers et al., 2012, Costa et al., 2011, Luheshi et al., 2010, Zhao et al., 2010, Rival et al., 2009, Luheshi et al., 2007, Crowther et al., 2005)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Alzheimer's disease 1

is ameliorated by MCU¹

(Twyning et al., 2024)

is ameliorated by Pdzd8^NIG.10362R

(Hewitt et al., 2022)

is ameliorated by stmA¹

(Zhang et al., 2017)

is ameliorated by stmA^rev499

(Zhang et al., 2017)

is exacerbated by Glut1^HMS02152

(Niccoli et al., 2016)

is ameliorated by Glut1^UAS.cNa

(Niccoli et al., 2016)

is exacerbated by Ldh^HMS00039

(Niccoli et al., 2021)

is exacerbated by Ldh^UAS.cNa

(Niccoli et al., 2021)

is exacerbated by crc^JF02007

(Niccoli et al., 2021)

is ameliorated by LanB1^EP-600

(Catterson et al., 2023)

is ameliorated by LanB1^UAS.mRFP1

(Catterson et al., 2023)

is ameliorated by LanB2^UAS.GFP

(Catterson et al., 2023)

is exacerbated by Xbp1^KK102632

(Catterson et al., 2023)

is ameliorated by Avic\GFP^{UAS.Tag:ER(KDEL)}

(Catterson et al., 2023)

is exacerbated by Avic\GFP^{UAS.Tag:SS(wg)}

(Catterson et al., 2023)

is ameliorated by Col4a1^UAS.mRFP1

(Catterson et al., 2023)

is ameliorated by LanA^EY02207

(Catterson et al., 2023)

is ameliorated by Hsap\LYZ^{UAS.Tag:SS(nec)}

(Sandin et al., 2016)

model of Alzheimer's disease

is ameliorated by Saur\spa^{2.ZAβ3.UAS.Tag:MYC,Tag:SS(nec)}

(Luheshi et al., 2010)

is ameliorated by Saur\spa^{ZAβ3.UAS.Tag:MYC,Tag:SS(nec)}

(Luheshi et al., 2010)

is ameliorated by Sod2^UAS.cMa

(Rival et al., 2009)

is ameliorated by Fer2LCH^UAS.cRa

(Rival et al., 2009)

is exacerbated by Sod1^UAS.cAa

(Rival et al., 2009)

is ameliorated by Sod1^RNAi.UAS.cHa

(Rival et al., 2009)

is ameliorated by GstS1^UASp.cWa

(Rival et al., 2009)

is ameliorated by Fer1HCH^UAS.cRa

(Rival et al., 2009)

is ameliorated by Cat^UAS.cAa

(Rival et al., 2009)

is ameliorated by Sod1ⁿ¹

(Rival et al., 2009)

is NOT exacerbated by per⁰¹

(Long et al., 2014)

is ameliorated by NT1^Cysknot.UAS

(Lim et al., 2015)

is ameliorated by sgg^S9E.UAS

(Sofola et al., 2010)

is ameliorated by tau^MR22

(Sofola et al., 2010)

is ameliorated by tau^EP3203

(Sofola et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

This allele represents a human variant implicated in disease.

(Crowther et al., 2005)

This construct carries the amyloid Aβ42 peptide; variant described relative to the full-length protein and in terms of the Aβ42 peptide.

APP:p.Glu693Gly

(FlyBase curators, 2019-)

Variants Synonym(s)

APP(Aβ42 peptide):p.Glu22Gly

Associated human disease model(s)

External database links

ClinVar: APP_18098

Comments concerning this variant

Phenotypic Data

Phenotypic Class

abnormal flight | progressive, with Scer\GAL4^shot-OK307

(Zhao et al., 2010)

abnormal locomotor behavior, with Scer\GAL4^elav-C155

(Rival et al., 2009)

abnormal locomotor behavior | adult stage, with Scer\GAL4^{elav.Switch.PO}

(Piper et al., 2014, Sofola-Adesakin et al., 2014)

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav-C155

(Lim et al., 2015)

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^elav.PU

(Long et al., 2014)

abnormal locomotor behavior | adult stage | progressive, with Scer\GAL4^nSyb.PS

(Hewitt et al., 2022)

abnormal locomotor behavior | adult stage | progressive | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Sofola et al., 2010)

abnormal locomotor behavior | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Rogers et al., 2012)

abnormal locomotor rhythm | adult stage | progressive, with Scer\GAL4^elav-C155

(Long et al., 2014)

abnormal neuroanatomy, with Scer\GAL4^elav.PU

(Long et al., 2014)

abnormal neurophysiology, with Scer\GAL4^shot-OK307

(Huang et al., 2013)

abnormal neurophysiology | adult stage | progressive, with Scer\GAL4^elav.PLu

(Zhao et al., 2010)

abnormal neurophysiology | adult stage | progressive, with Scer\GAL4^shot-OK307

(Zhao et al., 2010)

abnormal neurophysiology | progressive | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Sofola et al., 2010)

decreased rate of adult locomotory behavior | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Catterson et al., 2023)

decreased rate of feeding behavior | adult stage | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Catterson et al., 2023)

short lived, with Scer\GAL4^elav.PU

(Long et al., 2014)

short lived, with Scer\GAL4^{elav.Switch.PO}

(Sofola-Adesakin et al., 2014)

short lived, with Scer\GAL4^elav-C155

(Lim et al., 2015, Luheshi et al., 2010, Rival et al., 2009, Crowther et al., 2005)

short lived, with Scer\GAL4^shot-OK307

(Zhao et al., 2010)

short lived | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Catterson et al., 2023, Rogers et al., 2012, Sofola et al., 2010)

short lived | RU486 conditional, with Scer\GAL4^GS.nSyb.PB

(Catterson et al., 2023)

visible, with Scer\GAL4^elav-C155

(Crowther et al., 2005)

visible, with Scer\GAL4^GMR.PF

(Luheshi et al., 2010)

visible | adult stage, with Scer\GAL4^GMR.PF

(Costa et al., 2011)

Phenotype Manifest In

adult brain, with Scer\GAL4^elav.PU

(Long et al., 2014)

eye, with Scer\GAL4^elav-C155

(Crowther et al., 2005)

eye, with Scer\GAL4^GMR.PF

(Luheshi et al., 2010)

eye | adult stage, with Scer\GAL4^GMR.PF

(Costa et al., 2011)

mitochondrion | adult stage | progressive, with Scer\GAL4^shot-OK307

(Zhao et al., 2010)

synaptic vesicle | adult stage | progressive, with Scer\GAL4^shot-OK307

(Zhao et al., 2010)

Detailed Description

Statement

Reference

Expression of Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^elav-C155 significantly reduces the longevity of adult flies: their median survival is significantly decreased compared to controls at both 25[o]C and 29[o]C. It also reduces their locomotor abilities - their mean climbing velocity is lower than in controls and declines more rapidly with age.

(Lim et al., 2015)

The climbing ability of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^elav.PU declines more significantly with age compared with controls. This individuals are short lived compared to controls.

The brains of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^elav.PU show an increase in vacuole number compared to controls.

Flies expressing Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^elav.PU exhibit an impaired circadian activity rhythm in LD (12 house light, 12 hours dark) that becomes more severe with age. The average rhythm strength is also reduced in DD (total darkness).

The morphology and number of Pdf-positive flies is unaffected in the brains of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^elav.PU.

(Long et al., 2014)

Expression of Hsap\APP^{Arctic.Scer\UAS.T:SS-nec}, induced by RU486 thanks to expression under the control of the Scer\GAL4^{elav.Switch.PO} geneswitch system, is induced by lower doses of RU486 on a holidic (containing 50mM sucrose and 200mM biologically available nitrogen) diet compared to on a sugar-yeast diet. In a dose-response analysis, 25υM RU486 in the holidic medium reproduces both Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} expression levels and a similar defect in the flies' ability to perform negative geotaxis at old ages when receiving 200υM RU486 in sugar-yeast food.

(Piper et al., 2014)

Adult- onset expression of Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the Scer\GAL4^{elav.Switch.PO} driver (using RU486 food supplement to control the time of expression) significantly decreases adult lifespan and climbing abilities compared to controls.

(Sofola-Adesakin et al., 2014)

Expression of Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^OK307 induces an age-dependent decline in synaptic vesicle release probability.

Expression of Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^OK307 induces an age-dependent reduction in the density of presynaptic voltage-gated calcium channel clusters.

Expression of Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^OK307 induces an age-dependent increase in synaptic contact length.

(Huang et al., 2013)

Expression of Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^{elav.Switch.PO} (in combination with RU486 exposure to activate Gal4 expression) results in climbing defects and reduced lifespan, with increased severity upon longer RU486 exposure, as compared to untreated controls.

(Rogers et al., 2012)

Expression of Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the Scer\GAL4^GMR.PF driver results in a rough eye phenotype and a decrease in the adult eye size.

(Costa et al., 2011)

Flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155 display significantly increased survival when cultured on food containing 1000μ YM-F24 (an iron-specific metal chelator).

(Liu et al., 2011)

Expression of Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} in the brain under the control of Scer\GAL4^elav-C155 results in a drastic reduction in lifespan compared with controls.

(Luheshi et al., 2010)

Flies expressing Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^{elav.Switch.PO} from 2 day post-eclosion onwards (expression induced by feeding flies RU486) have a median lifespan that is shorter than controls by about 50%, and a maximum lifespan that is reduced by about 45%. Response latencies in the tergotrochanteral (TTM) and dorsal longitudinal (DLM) muscles following giant fibre stimulation are similar to controls at day 16, but are significantly increased at day 28. The stability of the TTM response to high frequency stimulation (100, 200 and 250 Hz) is inhibited. Age-related climbing behaviour is enhanced compared to controls, and this phenotype is rescued when the flies are fed lithium in adulthood.

(Sofola et al., 2010)

Expression of Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^shot-OK307 significantly accelerates the age-related decline in the flight ability of adult flies and shortens adult lifespan relative to controls. It also hastens the age-related decline in neurotransmission functionality: the success rate of eliciting excitatory junction potentials in the dorsal longitudinal muscle (DLM) fibers in response to brain stimulation is much lower compared to controls and the age-progressive increase in synaptic transmission fatigue rate is increased. Expression of Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} also leads to age-progressive depletion of synaptic vesicles, their increased size and heterogeneity, depletion of presynaptic mitochondria (as well as slightly higher frequency of damaged mitochondria) and accumulation of presynaptic vacuoles compared to age-matched controls. The number of axonal mitochondria is also reduced although their mean area is actually increased compared to controls and the rates of both anterograde and retrograde axonal transport in aged (but not young) adult flies is significantly decreased compared to age-matched controls.

The faster rate of age-related decline in neurotransmission along the the DLM pathway is also observed in flies expressing Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the Scer\GAL4^elav.PLu driver, although the decline rate is slower than with the Scer\GAL4^shot-OK307 driver.

(Zhao et al., 2010)

The metal chelator clioquinol reduces the toxic effects of Hsap\APP^{Arctic.Scer\UAS.T:nec}-driven Hsap\APP^{Scer\UAS.T:nec} overexpression.

(Rival et al., 2009)

Flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^unspecified have a median survival of about 8 days.

(Luheshi et al., 2007)

Expression of Hsap\APP^{Arctic.Scer\UAS.T:nec} driven by Scer\GAL4^elav-C155 results in a rough eye phenotype.

Flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155 show a dramatic decrease in longevity. Treatment with MK-801 markedly prolongs the survival of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.Feeding these flies with azo-dye Congo Red from the time of hatching results in a significant increase in longevity. Congo Red does not lengthen the lifespan of control flies.

(Crowther et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^GS.nSyb.PB has short lived | RU486 conditional phenotype, enhanceable by Xbp1^KK102632, Scer\GAL4^GS.nSyb.PB

(Catterson et al., 2023)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^GS.nSyb.PB has short lived | RU486 conditional phenotype, enhanceable by Avic\GFP^{UAS.Tag:SS(wg)}, Scer\GAL4^GS.nSyb.PB

(Catterson et al., 2023)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, enhanceable by NT1^Pro.UAS, Scer\GAL4^elav-C155

(Lim et al., 2015)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, enhanceable by Sod1^UAS.cAa, Scer\GAL4^elav-C155

(Rival et al., 2009)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has abnormal locomotor behavior phenotype, enhanceable by Sod1^UAS.cAa, Scer\GAL4^elav-C155

(Rival et al., 2009)

NOT Enhanced by

Statement

Reference

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav.PU has short lived phenotype, non-enhanceable by per⁰¹

(Long et al., 2014)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav.PU has abnormal neuroanatomy phenotype, non-enhanceable by per⁰¹

(Long et al., 2014)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav.PU has abnormal locomotor behavior | adult stage | progressive phenotype, non-enhanceable by per⁰¹

(Long et al., 2014)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, non-enhanceable by Saur\spa^{Z.UAS.Tag:MYC,Tag:SS(nec)}, Scer\GAL4^elav-C155

(Luheshi et al., 2010)

Suppressed by

Statement

Reference

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^{elav.Switch.PO} has short lived | RU486 conditional phenotype, suppressible by LanB1^EP-600, Scer\GAL4^{elav.Switch.PO}

(Catterson et al., 2023)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^{elav.Switch.PO} has short lived | RU486 conditional phenotype, suppressible by LanB2^HMC04076, Scer\GAL4^{elav.Switch.PO}

(Catterson et al., 2023)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^{elav.Switch.PO} has short lived | RU486 conditional phenotype, suppressible by LanB2^HMJ23628, Scer\GAL4^{elav.Switch.PO}

(Catterson et al., 2023)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^GS.nSyb.PB has short lived | RU486 conditional phenotype, suppressible by LanB1^UAS.mRFP1, Scer\GAL4^GS.nSyb.PB

(Catterson et al., 2023)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^{elav.Switch.PO} has decreased rate of adult locomotory behavior | RU486 conditional phenotype, suppressible by LanB1^EP-600, Scer\GAL4^{elav.Switch.PO}

(Catterson et al., 2023)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^{elav.Switch.PO} has decreased rate of feeding behavior | adult stage | RU486 conditional phenotype, suppressible by LanB1^EP-600, Scer\GAL4^{elav.Switch.PO}

(Catterson et al., 2023)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^{elav.Switch.PO} has short lived | RU486 conditional phenotype, suppressible by Col4a1^UAS.mRFP1, Scer\GAL4^{elav.Switch.PO}

(Catterson et al., 2023)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^{elav.Switch.PO} has short lived | RU486 conditional phenotype, suppressible | partially by LanA^EY02207, Scer\GAL4^{elav.Switch.PO}

(Catterson et al., 2023)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^GS.nSyb.PB has short lived | RU486 conditional phenotype, suppressible by LanB2^UAS.GFP, Scer\GAL4^GS.nSyb.PB

(Catterson et al., 2023)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^GS.nSyb.PB has short lived | RU486 conditional phenotype, suppressible by Avic\GFP^{UAS.Tag:ER(KDEL)}, Scer\GAL4^GS.nSyb.PB

(Catterson et al., 2023)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^{elav.Switch.PO} has short lived | RU486 conditional phenotype, suppressible by Avic\GFP^{UAS.Tag:ER(KDEL)}, Scer\GAL4^{elav.Switch.PO}

(Catterson et al., 2023)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^nSyb.PS has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible | partially by Pdzd8^NIG.10362R, Scer\GAL4^nSyb.PS

(Hewitt et al., 2022)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by NT1^Cysknot.UAS, Scer\GAL4^elav-C155

(Lim et al., 2015)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has abnormal locomotor behavior | adult stage phenotype, suppressible by NT1^Cysknot.UAS, Scer\GAL4^elav-C155

(Lim et al., 2015)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has abnormal locomotor behavior | adult stage phenotype, suppressible by NT1^fl.UAS.EGFP, Scer\GAL4^elav-C155

(Lim et al., 2015)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by Saur\spa^{ZAβ3.UAS.Tag:MYC,Tag:SS(nec)}, Scer\GAL4^elav-C155

(Luheshi et al., 2010)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by Saur\spa^{2.ZAβ3.UAS.Tag:MYC,Tag:SS(nec)}, Scer\GAL4^elav-C155

(Luheshi et al., 2010)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^GMR.PF has visible phenotype, suppressible by Saur\spa^{2.ZAβ3.UAS.Tag:MYC,Tag:SS(nec)}, Scer\GAL4^GMR.PF

(Luheshi et al., 2010)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^{elav.Switch.PO} has short lived | RU486 conditional phenotype, suppressible | partially by sgg^S9E.UAS, Scer\GAL4^{elav.Switch.PO}

(Sofola et al., 2010)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^{elav.Switch.PO} has abnormal locomotor behavior | adult stage | progressive | RU486 conditional phenotype, suppressible by sgg^S9E.UAS, Scer\GAL4^{elav.Switch.PO}

(Sofola et al., 2010)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^{elav.Switch.PO} has abnormal locomotor behavior | adult stage | progressive | RU486 conditional phenotype, suppressible by tau^MR22/tau^EP3203

(Sofola et al., 2010)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by P{GSV1}1774, Scer\GAL4^elav-C155

(Rival et al., 2009)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by Fer1HCH^UAS.cRa, Scer\GAL4^elav-C155

(Rival et al., 2009)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by Fer2LCH^UAS.cRa, Scer\GAL4^elav-C155

(Rival et al., 2009)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by Cat^UAS.cAa, Scer\GAL4^elav-C155

(Rival et al., 2009)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by Sod2^UAS.cMa, Scer\GAL4^elav-C155

(Rival et al., 2009)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by sni^UAS.cBa, Scer\GAL4^elav-C155

(Rival et al., 2009)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by GstS1^UASp.cWa, Scer\GAL4^elav-C155

(Rival et al., 2009)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by Sod1^RNAi.UAS.cHa, Scer\GAL4^elav-C155

(Rival et al., 2009)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by Sod1ⁿ¹/Sod[+]

(Rival et al., 2009)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has abnormal locomotor behavior phenotype, suppressible by Fer1HCH^UAS.cRa, Scer\GAL4^elav-C155

(Rival et al., 2009)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has abnormal locomotor behavior phenotype, suppressible by Fer2LCH^UAS.cRa, Scer\GAL4^elav-C155

(Rival et al., 2009)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has abnormal locomotor behavior phenotype, suppressible by Cat^UAS.cAa, Scer\GAL4^elav-C155

(Rival et al., 2009)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has abnormal locomotor behavior phenotype, suppressible by Sod2^UAS.cMa, Scer\GAL4^elav-C155

(Rival et al., 2009)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by P{GSV1}811, Scer\GAL4^elav-C155

(Rival et al., 2009)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, suppressible by P{GSV1}1357, Scer\GAL4^elav-C155

(Rival et al., 2009)

NOT suppressed by

Statement

Reference

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, non-suppressible by NT1^fl.UAS.EGFP, Scer\GAL4^elav-C155

(Lim et al., 2015)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has abnormal locomotor behavior | adult stage phenotype, non-suppressible by NT1^Pro.UAS, Scer\GAL4^elav-C155

(Lim et al., 2015)

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav-C155 has short lived phenotype, non-suppressible by Saur\spa^{Z.UAS.Tag:MYC,Tag:SS(nec)}, Scer\GAL4^elav-C155

(Luheshi et al., 2010)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^elav.PU has adult brain phenotype, non-enhanceable by per⁰¹

(Long et al., 2014)

Suppressed by

Statement

Reference

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Saur\spa^{2.ZAβ3.UAS.Tag:MYC,Tag:SS(nec)}, Scer\GAL4^GMR.PF

(Luheshi et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The short lifespan of adult flies expressing Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^elav-C155 (cultured at either 25[o]C or 29[o]C) is significantly lengthened by co-expression of NT1^{Cysknot.Scer\UAS} and decreased further by co-expression of NT1^Pro.Scer\UAS. Co-expression of NT1^{fl.Scer\UAS.T:Avic\GFP-EGFP} or of NT1^GD10831 RNAi has no significant effect on the lifespan of Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} expressing flies and neither has the combination with a single copy of NT1^unspecified loss of function allele.

The impaired locomotor ability of adult flies expressing Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^elav-C155 is partially ameliorated by co-expression of either NT1^{Cysknot.Scer\UAS} or NT1^{fl.Scer\UAS.T:Avic\GFP-EGFP}. Co-expression of NT1^Pro.Scer\UAS or of NT1^GD10831 RNAi has no significant effect on the climbing ability of Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} expressing flies and neither has the combination with a single copy of NT1^unspecified loss of function allele.

(Lim et al., 2015)

per⁰¹ does not enhance the climbing defects seen when Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} is expressed under the control of Scer\GAL4^elav.PU.

per⁰¹ does not further enhance the increase in vacuole number seen when Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} is expressed under the control of Scer\GAL4^elav.PU.

(Long et al., 2014)

Expression of Fer1HCH^VDRC.cUa in flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155 reduces hatching efficiency.

Expression of Fer2LCH^VDRC.cUa in flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155 reduces hatching efficiency.

(Liu et al., 2011)

Flies co-expressing Saur\spa^{ZAβ3.Scer\UAS.T:Hsap\MYC,T:SS-nec} with Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} via Scer\GAL4^elav-C155 live longer than flies expressing Scer\GAL4^elav-C155>Hsap\APP^{Arctic.Scer\UAS.T:SS-nec}.

Flies co-expressing Saur\spa^{2.ZAβ3.Scer\UAS.T:Hsap\MYC,T:SS-nec} with Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^elav-C155 live almost as long as wild-type controls.

Co-expression of Saur\spa^{Z.Scer\UAS.T:Hsap\MYC,T:SS-nec} under the control of Scer\GAL4^elav-C155 does not affect the viability of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:SS-nec}.

Co-expression of Saur\spa^{2.ZAβ3.Scer\UAS.T:Hsap\MYC,T:SS-nec} using Scer\GAL4^GMR.PF restores the eye-morphology in flies expressing Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} alone.

(Luheshi et al., 2010)

Expression of sgg^S9E.Scer\UAS partially suppresses the decrease in median and maximum lifespan seen in flies expressing Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^{elav.Switch.PO} from 2 day post-eclosion onwards (expression induced by feeding flies RU486). The climbing behaviour defects are fully rescued.

tau^MR22/tau^EP3203 suppresses the climbing defects seen in flies expressing Hsap\APP^{Arctic.Scer\UAS.T:SS-nec} under the control of Scer\GAL4^{elav.Switch.PO} from 2 day post-eclosion onwards (expression induced by feeding flies RU486). This rescue is further enhanced by feeding the flies lithium in adulthood.

(Sofola et al., 2010)

Activation of P{GSV1}811 with Scer\GAL4^elav-C155 in flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} suppresses the decreased survival phenotype of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Activation of P{GSV1}1357 with Scer\GAL4^elav-C155 in flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} suppresses the decreased survival phenotype of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Activation of P{GSV1}1774 with Scer\GAL4^elav-C155 in flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} suppresses the decreased survival phenotype of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Overexpression of Fer1HCH^Scer\UAS.cRa suppresses the decreased survival phenotype of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Overexpression of Fer1HCH^Scer\UAS.cRa suppresses the decline in locomotor function of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Overexpression of Fer2LCH^Scer\UAS.cRa suppresses the decreased survival phenotype of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Overexpression of Fer2LCH^Scer\UAS.cRa suppresses the decline in locomotor function of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Overexpression of Cat^Scer\UAS.cAa suppresses the decreased survival phenotype of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Overexpression of Cat^Scer\UAS.cAa suppresses the decline in locomotor function of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Overexpression of Sod2^Scer\UAS.cMa suppresses the decreased survival phenotype of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Overexpression of Sod2^Scer\UAS.cMa suppresses the decline in locomotor function of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Overexpression of Sod^Scer\UAS.cAa enhances the decreased survival phenotype of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Overexpression of Sod^Scer\UAS.cAa accelerates the decline in locomotor function of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Overexpression of sni^Scer\UAS.cBa partially suppresses the decreased survival phenotype of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Overexpression of GstS1^{Scer\UAS.P\T.cWa} partially suppresses the decreased survival phenotype of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Overexpression of Sod^{dsRNA.Scer\UAS.cHa} suppresses the decreased survival phenotype of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

Sodⁿ¹ dominantly suppresses the decreased survival phenotype of flies expressing Hsap\APP^{Arctic.Scer\UAS.T:nec} under the control of Scer\GAL4^elav-C155.

(Rival et al., 2009)

Complementation and Rescue Data

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Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Arctic Aβ42

(Costa et al., 2011)

Aβ42^E22G

(Briffa et al., 2017)

Hsap\APP^{Arctic.Scer\UAS.T:SS-nec}

Hsap\APP^{Arctic.Scer\UAS.T:nec}

Hsap\APP^{Arctic.UAS.Tag:SS(nec)}

Name Synonyms

Secondary FlyBase IDs

References (36)

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