FlyBase Allele Report: Dmel\Snr1[HMS00363]

General Information

Symbol

Dmel\Snr1^HMS00363

Species

D. melanogaster

Name

FlyBase ID

FBal0248128

Feature type

allele

Associated gene

Dmel\Snr1

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00363}

Also Known As

snr1 RNAi, UAS-Snr1^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00363}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00363

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of rhabdoid cancer

CEA

(Xie et al., 2017)

CEA with BacA\p35^UAS.cHa

(Xie et al., 2017)

model of Coffin-Siris syndrome 3

CEA

(MacPherson et al., 2023, Chubak et al., 2019)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Snr1^HMS00363 under the control of Scer\GAL4^insc-Mz1407 leads to a severe increase in the number of type II neuroblasts per third instar larval brain lobe coupled to brain enlargement, as compared to controls.

(Liu et al., 2017)

Somatic (flip-out) clones of cells expressing Snr1^HMS00363 under the control of Scer\GAL4^Act.PU are highly apoptotic in the wing pouch but cause mainly cell non-autonomous increase in proliferation and tissue overgrowth in the notum region of third instar larval wing disc.

(Xie et al., 2017)

Expression of Snr1^HMS00363 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} does not result in melanisation of the fat body.

(Zang et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Act.PU, Snr1^HMS00363 has abnormal size | third instar larval stage | somatic clone phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^Act.PU

(Xie et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, Snr1^HMS00363 has increased cell number | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/Su(Tpl)^HMS00277

(Liu et al., 2017)

Scer\GAL4^insc-Mz1407, Snr1^HMS00363 has abnormal size | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/Su(Tpl)^HMS00277

(Liu et al., 2017)

Scer\GAL4^insc-Mz1407, Snr1^HMS00363 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/Su(Tpl)^HMS00277

(Liu et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363 has neoplasia | somatic clone | third instar larval stage phenotype, suppressible by puc^UAS.cMa, Scer\GAL4^Act.PU

(Xie et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363 has neoplasia | somatic clone | third instar larval stage phenotype, suppressible by bsk^DN.UAS.cUa, Scer\GAL4^Act.PU

(Xie et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363 has neoplasia | somatic clone | third instar larval stage phenotype, suppressible by Stat92E^RNAi.UAS, Scer\GAL4^Act.PU

(Xie et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363 has neoplasia | somatic clone | third instar larval stage phenotype, suppressible by N^JF02959, Scer\GAL4^Act.PU

(Xie et al., 2017)

NOT suppressed by

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363 has neoplasia | somatic clone | third instar larval stage phenotype, non-suppressible by Ilp8^GD1670, Scer\GAL4^Act.PU

(Xie et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363 has neoplasia | somatic clone | third instar larval stage phenotype, non-suppressible by Mmp1^JF01336, Scer\GAL4^Act.PU

(Xie et al., 2017)

Other

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, Snr1^HMS00363, dhd^+t4.3, dhd^J5 has female sterile phenotype

(Torres-Campana et al., 2022)

BacA\p35^UAS.cHa, Scer\GAL4^en.PU, Snr1^HMS00363 has abnormal developmental rate | larval stage phenotype

(Xie et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^en.PU, Snr1^HMS00363 has neoplasia | third instar larval stage phenotype

(Xie et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^ptc.PU, Snr1^HMS00363 has abnormal developmental rate | larval stage phenotype

(Xie et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^ptc.PU, Snr1^HMS00363 has neoplasia | third instar larval stage phenotype

(Xie et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Act.PU, Snr1^HMS00363 has wing pouch | third instar larval stage | somatic clone phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^Act.PU

(Xie et al., 2017)

Scer\GAL4^Act.PU, Snr1^HMS00363 has wing disc | third instar larval stage | somatic clone phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^Act.PU

(Xie et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^insc-Mz1407, Snr1^HMS00363 has type II neuroblast | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^insc-Mz1407/Su(Tpl)^HMS00277

(Liu et al., 2017)

Scer\GAL4^insc-Mz1407, Snr1^HMS00363 has larval brain | third instar larval stage phenotype, suppressible | partially by Scer\GAL4^insc-Mz1407/Su(Tpl)^HMS00277

(Liu et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363 has wing disc | somatic clone | third instar larval stage phenotype, suppressible by Stat92E^RNAi.UAS, Scer\GAL4^Act.PU

(Xie et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363 has wing disc | somatic clone | third instar larval stage phenotype, suppressible by N^JF02959, Scer\GAL4^Act.PU

(Xie et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363 has wing disc | somatic clone | third instar larval stage phenotype, suppressible by puc^UAS.cMa, Scer\GAL4^Act.PU

(Xie et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363 has wing disc | somatic clone | third instar larval stage phenotype, suppressible by bsk^DN.UAS.cUa, Scer\GAL4^Act.PU

(Xie et al., 2017)

NOT suppressed by

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363 has wing disc | somatic clone | third instar larval stage phenotype, non-suppressible by Ilp8^GD1670, Scer\GAL4^Act.PU

(Xie et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363 has wing disc | somatic clone | third instar larval stage phenotype, non-suppressible by Mmp1^JF01336, Scer\GAL4^Act.PU

(Xie et al., 2017)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^Act.PU, Snr1^HMS00363 has eye disc | somatic clone | third instar larval stage phenotype

(Xie et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^en.PU, Snr1^HMS00363 has wing disc | third instar larval stage phenotype

(Xie et al., 2017)

BacA\p35^UAS.cHa, Scer\GAL4^ptc.PU, Snr1^HMS00363 has wing disc | third instar larval stage phenotype

(Xie et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of type II neuroblasts and brain enlargement in third instar larval brain lobes resulting from the expression of Snr1^HMS00363 under the control of Scer\GAL4^insc-Mz1407 is almost fully suppressed by the co-expression of Su(Tpl)^HMS00277.

(Liu et al., 2017)

Xenogenetic Interactions

Statement

Reference

Co-expression of Snr1^HMS00363 and BacA\p35^Scer\UAS.cHa driven by Scer\GAL4^Act.PU in somatic (flip-out) clones in either wing or eye discs of third instar larvae leads to aggressive tissue overgrowth. This overgrowth is (in the wing discs) significantly suppressed by co-expression of any of the following: Stat92E^{dsRNA.Scer\UAS}, N^JF02959, puc^Scer\UAS.cMa or bsk^{DN.Scer\UAS.cUa} but not by co-expression of either Ilp8^GD1670 or Mmp1^JF01336.

Massive tissue overgrowth is also observed in wing discs expressing the Snr1^HMS00363 and BacA\p35^Scer\UAS.cHa under the control of either Scer\GAL4^en.PU or Scer\GAL4^ptc.PU, this also leads to developmental delay as some of the animals stay in larval stage for up to 10 days.

(Xie et al., 2017)

Complementation and Rescue Data

Rescued by

Snr1^HMS00363 is rescued by Scer\GAL4^Act.PU/Snr1^UASp.Tag:HA

(Xie et al., 2017)

Partially rescued by

Snr1^HMS00363 is partially rescued by Scer\GAL4^Act.PU/Snr1^{ΔC.UASp.Tag:HA}

(Xie et al., 2017)

Not rescued by

Snr1^HMS00363 is not rescued by Scer\GAL4^Act.PU/Snr1^{ΔNES.UASp.Tag:HA}

(Xie et al., 2017)

Comments

Co-expression of Snr1^HMS00363 and BacA\p35^Scer\UAS.cHa driven by Scer\GAL4^Act.PU in somatic (flip-out) clones in either wing discs of third instar larvae leads to aggressive tissue overgrowth, which can be rescued by expression of Snr1^{Scer\UAS.P\T.T:Ivir\HA1}, partially rescued by expression Snr1^{ΔC.Scer\UAS.P\T.T:Ivir\HA1} but cannot be rescued by expression of Snr1^{ΔNES.Scer\UAS.P\T.T:Ivir\HA1}.

(Xie et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

32372

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00363}attP2

NIG-Fly

HMS00363

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00363}attP2

Notes on Origin