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General Information
Symbol
Dmel\par-1HMS00405
Species
D. melanogaster
Name
FlyBase ID
FBal0248166
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Par-1 RNAi, HMS00405
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
UAS regulatory sequences drive expression of a short inverted repeat.
Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Expression of par-1HMS00405 under the control of Scer\GAL4ppk.PG does not affect the morphology or axonal projections of larval class IV dendritic arborizing neuron (c4da) in the ventral nerve cord but it leads to dendrite pruning defects in the pupal stage as no dendrite thinning is observed at 6h after puparium formation (APF) and the neurons retain dendrites attached to the cell body at 18h APF (by which point the pruning is completed in wild-type flies).
Embryos from mothers expressing par-1HMS00405 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 display enlarged cells with multiple centrosomes.
Expression of par-1HMS00405 under the control of Scer\GAL4SPARC-MI00329-GAL4 does not result in melanisation of the fat body.
Females heterozygous for par-1HMS00405 and MTD-Gal4 (consists of Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16) crossed to par-1HMS00405 males results in: "few eggs,47% hatching"; egg/embryonic phenotype: few eggs and tiny; maternal effect; oogenesis defect.
Expression of par-1HMS00405 under the control of either Scer\GAL4ey.PH or Scer\GAL4Bx-MS1096 reduces the size of the eye or wing respectively. When par-1HMS00405 is expressed under the control of Scer\GAL4hh-Gal4 the relative P-compartment area of the wing is reduced compared to controls. Expressing par-1HMS00405 under the control of Scer\GAL4GMR.PU results in increased eye disc apoptosis. Eye disc clones expressing par-1HMS00405 under the control of Scer\GAL4unspecified are significantly smaller than the corresponding wild type controls.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
Scer\GAL4ppk.PG, par-1HMS00405 has dendrite | pupal stage phenotype, enhanceable by IKKepsilon[+]/IKKεalice
NOT Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
The penetrance of the pruning defects observed in class IV dendritic arborizing neurons of pupae expressing par-1HMS00405 under the control of Scer\GAL4ppk.PG is significantly enhanced by co-expression of any of the following: tauScer\UAS.RA.T:Ivir\HA1, tauS184A.Scer\UAS.RA.T:Ivir\HA1, kat-60L1HMS00510 or shi1.Scer\UAS (the latter two on their own induce mild pruning defects too) or by combination with IKKεalice. The phenotype is weakly enhanced by co-expression of futschEP1419 and can be suppressed by combination with a single copy of tauMR22, while combination with one copy of any of the following: futschK68, EcR31, Rpn8k08003 fails to significantly modify the phenotype.
Maternal expression of par-1HMS00405 under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 rescues the abnormal chromosome positioning of embryos from aPKCts/Df(2R)l4 mothers, but also results in enlarged cells with multiple centrosomes.
Expression of ykiScer\UAS.cZa suppresses the shrunken P-compartment phenotype seen in wing discs when par-1HMS00405 is expressed under the control of Scer\GAL4hh-Gal4. Expression of par-1HMS00405 suppresses the increased P-compartment phenotype seen in wing discs when exGD12782 is expressed under the control of Scer\GAL4hh-Gal4. Expression of par-1HMS00405 suppresses the wing overgrowth seen when exGD12782 is expressed under the control of Scer\GAL4Bx-MS1096. Expression of par-1HMS00405 suppresses the overgrowth phenotype seen in wings expressing ftGD881 under the control of Scer\GAL4Bx-MS1096. wtsx1 rescues the reduction in eye disc clone size seen when par-1HMS00405 is expressed under the control of Scer\GAL4unspecified, with the resulting clones similar in size to those seen with wtsx1 alone. Expression of par-1HMS00405 under the control of Scer\GAL4unspecified does not suppress the tumorous overgrowth seen in hpoBF33 mutant eye disc clones. Expression of par-1HMS00405 under the control of Scer\GAL4unspecified does not suppress the eye overgrowth seen in savSH13 mutant eye clones.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
par-1HMS00405
Name Synonyms
Secondary FlyBase IDs
    References (8)