FlyBase Allele Report: Dmel\par-1[HMS00405]

General Information

Symbol

Dmel\par-1^HMS00405

Species

D. melanogaster

Name

FlyBase ID

FBal0248166

Feature type

allele

Associated gene

Dmel\par-1

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00405}

Also Known As

Par-1 RNAi, HMS00405

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00405}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00405

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

centrosome | embryonic stage | maternal effect | increased number, with Scer\GAL4^{VP16.mat.αTub67C}

(Jiang et al., 2015)

dendrite, with Scer\GAL4^ppk.PU

(Bu et al., 2022)

dendrite | pupal stage, with Scer\GAL4^ppk.PG

(Herzmann et al., 2017)

dendritic microtubule, with Scer\GAL4⁴⁷⁷

(Bu et al., 2022)

dendritic microtubule, with Scer\GAL4^ppk.PU

(Bu et al., 2022)

embryo | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Sopko et al., 2014)

eye, with Scer\GAL4^ey.PH

(Huang et al., 2013)

eye disc, with Scer\GAL4^GMR.PU

(Huang et al., 2013)

eye disc | somatic clone, with Scer\GAL4^unspecified

(Huang et al., 2013)

garland cell, with Scer\GAL4^sns.PK

(Heiden et al., 2021)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4⁴⁷⁷

(Bu et al., 2022)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.PU

(Bu et al., 2022)

larval multidendritic class IV neuron | pupal stage, with Scer\GAL4^ppk.PG

(Herzmann et al., 2017)

slit diaphragm | decreased number, with Scer\GAL4^sns.PK

(Heiden et al., 2021)

wing, with Scer\GAL4^Bx-MS1096

(Huang et al., 2013)

wing disc, with Scer\GAL4^hh-Gal4

(Huang et al., 2013)

Detailed Description

Statement

Reference

Expression of par-1^HMS00405 under the control of Scer\GAL4^ppk.PG does not affect the morphology or axonal projections of larval class IV dendritic arborizing neuron (c4da) in the ventral nerve cord but it leads to dendrite pruning defects in the pupal stage as no dendrite thinning is observed at 6h after puparium formation (APF) and the neurons retain dendrites attached to the cell body at 18h APF (by which point the pruning is completed in wild-type flies).

(Herzmann et al., 2017)

Embryos from mothers expressing par-1^HMS00405 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} display enlarged cells with multiple centrosomes.

(Jiang et al., 2015)

Expression of par-1^HMS00405 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} does not result in melanisation of the fat body.

(Zang et al., 2015)

Females heterozygous for par-1^HMS00405 and MTD-Gal4 (consists of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16}) crossed to par-1^HMS00405 males results in: "few eggs,47% hatching"; egg/embryonic phenotype: few eggs and tiny; maternal effect; oogenesis defect.

(Sopko et al., 2014)

Expression of par-1^HMS00405 under the control of either Scer\GAL4^ey.PH or Scer\GAL4^Bx-MS1096 reduces the size of the eye or wing respectively. When par-1^HMS00405 is expressed under the control of Scer\GAL4^hh-Gal4 the relative P-compartment area of the wing is reduced compared to controls. Expressing par-1^HMS00405 under the control of Scer\GAL4^GMR.PU results in increased eye disc apoptosis.

Eye disc clones expressing par-1^HMS00405 under the control of Scer\GAL4^unspecified are significantly smaller than the corresponding wild type controls.

(Huang et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ppk.PG, par-1^HMS00405 has abnormal neuroanatomy | pupal stage phenotype, enhanceable by tau^{UAS.RA.Tag:HA}/Scer\GAL4^ppk.PG

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has abnormal neuroanatomy | pupal stage phenotype, enhanceable by IKKepsilon[+]/IKKε^alice

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has abnormal neuroanatomy | pupal stage phenotype, enhanceable by tau^{S184A.UAS.RA.Tag:HA}/Scer\GAL4^ppk.PG

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has abnormal neuroanatomy | pupal stage phenotype, enhanceable by futsch^EP1419/Scer\GAL4^ppk.PG

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has abnormal neuroanatomy | pupal stage phenotype, enhanceable by shi^1.UAS/Scer\GAL4^ppk.PG

(Herzmann et al., 2017)

NOT Enhanced by

Statement

Reference

Scer\GAL4^ppk.PU, par-1^HMS00405 has abnormal neuroanatomy | P-stage phenotype, non-enhanceable by chb^GL00367/Scer\GAL4^ppk.PU

(Bu et al., 2022)

Scer\GAL4^ppk.PG, par-1^HMS00405 has abnormal neuroanatomy | pupal stage phenotype, non-enhanceable by EcR³¹/EcR[+]

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has abnormal neuroanatomy | pupal stage phenotype, non-enhanceable by Rpn8[+]/Rpn8^k08003

(Herzmann et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^ppk.PG, par-1^HMS00405 has abnormal neuroanatomy | pupal stage phenotype, suppressible by tau^MR22/tau[+]

(Herzmann et al., 2017)

NOT suppressed by

Statement

Reference

Scer\GAL4^ppk.PU, par-1^HMS00405 has abnormal neuroanatomy | P-stage phenotype, non-suppressible by chb^GL00367/Scer\GAL4^ppk.PU

(Bu et al., 2022)

Scer\GAL4^ppk.PG, par-1^HMS00405 has abnormal neuroanatomy | pupal stage phenotype, non-suppressible by futsch^K68/futsch[+]

(Herzmann et al., 2017)

Enhancer of

Statement

Reference

par-1^HMS00405/Scer\GAL4^ppk.PG is an enhancer of abnormal neuroanatomy | pupal stage phenotype of Scer\GAL4^ppk.PG, shi^1.UAS

(Herzmann et al., 2017)

par-1^HMS00405/Scer\GAL4^ppk.PG is an enhancer of abnormal neuroanatomy | pupal stage phenotype of Scer\GAL4^ppk.PG, kat-60L1^HMS00510

(Herzmann et al., 2017)

Suppressor of

Statement

Reference

par-1^HMS00405/Scer\GAL4^ppk.PU is a suppressor of abnormal neuroanatomy | P-stage phenotype of Scer\GAL4^ppk.PU, chb^GL00367

(Bu et al., 2022)

par-1^HMS00405/Scer\GAL4^Bx-MS1096 is a suppressor of visible phenotype of ex^GD12782

(Huang et al., 2013)

Other

Statement

Reference

aPKC^ts/Df(2R)l4, Scer\GAL4^{VP16.mat.αTub67C}, par-1^HMS00405 has increased cell size | maternal effect | embryonic stage phenotype

(Jiang et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ppk.PG, par-1^HMS00405 has larval multidendritic class IV neuron | pupal stage phenotype, enhanceable by tau^{UAS.RA.Tag:HA}/Scer\GAL4^ppk.PG

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has larval multidendritic class IV neuron | pupal stage phenotype, enhanceable by shi^1.UAS/Scer\GAL4^ppk.PG

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has dendrite | pupal stage phenotype, enhanceable by shi^1.UAS/Scer\GAL4^ppk.PG

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has dendrite | pupal stage phenotype, enhanceable by tau^{UAS.RA.Tag:HA}/Scer\GAL4^ppk.PG

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has larval multidendritic class IV neuron | pupal stage phenotype, enhanceable by IKKepsilon[+]/IKKε^alice

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has dendrite | pupal stage phenotype, enhanceable by IKKepsilon[+]/IKKε^alice

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has larval multidendritic class IV neuron | pupal stage phenotype, enhanceable by tau^{S184A.UAS.RA.Tag:HA}/Scer\GAL4^ppk.PG

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has dendrite | pupal stage phenotype, enhanceable by tau^{S184A.UAS.RA.Tag:HA}/Scer\GAL4^ppk.PG

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has larval multidendritic class IV neuron | pupal stage phenotype, enhanceable by futsch^EP1419/Scer\GAL4^ppk.PG

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has dendrite | pupal stage phenotype, enhanceable by futsch^EP1419/Scer\GAL4^ppk.PG

(Herzmann et al., 2017)

NOT Enhanced by

Statement

Reference

Scer\GAL4^ppk.PG, par-1^HMS00405 has larval multidendritic class IV neuron | pupal stage phenotype, non-enhanceable by EcR³¹/EcR[+]

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has dendrite | pupal stage phenotype, non-enhanceable by EcR³¹/EcR[+]

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has larval multidendritic class IV neuron | pupal stage phenotype, non-enhanceable by Rpn8[+]/Rpn8^k08003

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has dendrite | pupal stage phenotype, non-enhanceable by Rpn8[+]/Rpn8^k08003

(Herzmann et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^ppk.PU, par-1^HMS00405 has dendritic microtubule phenotype, suppressible by chb^GL00367/Scer\GAL4^ppk.PU

(Bu et al., 2022)

Scer\GAL4^ppk.PG, par-1^HMS00405 has larval multidendritic class IV neuron | pupal stage phenotype, suppressible by tau^MR22/tau[+]

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has dendrite | pupal stage phenotype, suppressible by tau^MR22/tau[+]

(Herzmann et al., 2017)

Scer\GAL4^hh-Gal4, par-1^HMS00405 has wing disc phenotype, suppressible by yki^UAS.cZa/Scer\GAL4^hh-Gal4

(Huang et al., 2013)

Scer\GAL4^unspecified, par-1^HMS00405 has eye disc | somatic clone phenotype, suppressible by wts^x1

(Huang et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^ppk.PG, par-1^HMS00405 has larval multidendritic class IV neuron | pupal stage phenotype, non-suppressible by futsch^K68/futsch[+]

(Herzmann et al., 2017)

Scer\GAL4^ppk.PG, par-1^HMS00405 has dendrite | pupal stage phenotype, non-suppressible by futsch^K68/futsch[+]

(Herzmann et al., 2017)

Enhancer of

Statement

Reference

par-1^HMS00405/Scer\GAL4^ppk.PG is an enhancer of larval multidendritic class IV neuron | pupal stage phenotype of Scer\GAL4^ppk.PG, shi^1.UAS

(Herzmann et al., 2017)

par-1^HMS00405/Scer\GAL4^ppk.PG is an enhancer of larval multidendritic class IV neuron | pupal stage phenotype of Scer\GAL4^ppk.PG, kat-60L1^HMS00510

(Herzmann et al., 2017)

Suppressor of

Statement

Reference

par-1^HMS00405/Scer\GAL4^ppk.PU is a suppressor of microtubule phenotype of Scer\GAL4^ppk.PU, chb^GL00367

(Bu et al., 2022)

par-1^HMS00405/Scer\GAL4^ppk.PU is a suppressor of larval dorsal multidendritic neuron ddaC phenotype of Scer\GAL4^ppk.PU, chb^GL00367

(Bu et al., 2022)

par-1^HMS00405/Scer\GAL4^ppk.PU is a suppressor of dendritic microtubule phenotype of Scer\GAL4^ppk.PU, chb^GL00367

(Bu et al., 2022)

par-1^HMS00405/Scer\GAL4^{VP16.mat.αTub67C} is a suppressor of centrosome | maternal effect | embryonic stage phenotype of aPKC^ts/Df(2R)l4

(Jiang et al., 2015)

par-1^HMS00405/Scer\GAL4^Bx-MS1096 is a suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, ex^GD12782

(Huang et al., 2013)

par-1^HMS00405/Scer\GAL4^Bx-MS1096 is a suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, ft^GD881

(Huang et al., 2013)

par-1^HMS00405/Scer\GAL4^hh-Gal4 is a suppressor of wing disc phenotype of Scer\GAL4^hh-Gal4, ex^GD12782

(Huang et al., 2013)

NOT Suppressor of

Statement

Reference

par-1^HMS00405/Scer\GAL4^unspecified is a non-suppressor of eye | somatic clone phenotype of sav^SH13

(Huang et al., 2013)

par-1^HMS00405/Scer\GAL4^unspecified is a non-suppressor of eye disc | somatic clone phenotype of hpo^BF33

(Huang et al., 2013)

par-1^HMS00405/Scer\GAL4^unspecified is a non-suppressor of eye disc | somatic clone phenotype of wts^x1

(Huang et al., 2013)

Other

Statement

Reference

aPKC^ts/Df(2R)l4, Scer\GAL4^{VP16.mat.αTub67C}, par-1^HMS00405 has centrosome | increased number | maternal effect | embryonic stage phenotype

(Jiang et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance of the pruning defects observed in class IV dendritic arborizing neurons of pupae expressing par-1^HMS00405 under the control of Scer\GAL4^ppk.PG is significantly enhanced by co-expression of any of the following: tau^{Scer\UAS.RA.T:Ivir\HA1}, tau^{S184A.Scer\UAS.RA.T:Ivir\HA1}, kat-60L1^HMS00510 or shi^1.Scer\UAS (the latter two on their own induce mild pruning defects too) or by combination with IKKε^alice. The phenotype is weakly enhanced by co-expression of futsch^EP1419 and can be suppressed by combination with a single copy of tau^MR22, while combination with one copy of any of the following: futsch^K68, EcR³¹, Rpn8^k08003 fails to significantly modify the phenotype.

(Herzmann et al., 2017)

Maternal expression of par-1^HMS00405 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} rescues the abnormal chromosome positioning of embryos from aPKC^ts/Df(2R)l4 mothers, but also results in enlarged cells with multiple centrosomes.

(Jiang et al., 2015)

Expression of yki^Scer\UAS.cZa suppresses the shrunken P-compartment phenotype seen in wing discs when par-1^HMS00405 is expressed under the control of Scer\GAL4^hh-Gal4.

Expression of par-1^HMS00405 suppresses the increased P-compartment phenotype seen in wing discs when ex^GD12782 is expressed under the control of Scer\GAL4^hh-Gal4.

Expression of par-1^HMS00405 suppresses the wing overgrowth seen when ex^GD12782 is expressed under the control of Scer\GAL4^Bx-MS1096.

Expression of par-1^HMS00405 suppresses the overgrowth phenotype seen in wings expressing ft^GD881 under the control of Scer\GAL4^Bx-MS1096.

wts^x1 rescues the reduction in eye disc clone size seen when par-1^HMS00405 is expressed under the control of Scer\GAL4^unspecified, with the resulting clones similar in size to those seen with wts^x1 alone.

Expression of par-1^HMS00405 under the control of Scer\GAL4^unspecified does not suppress the tumorous overgrowth seen in hpo^BF33 mutant eye disc clones.

Expression of par-1^HMS00405 under the control of Scer\GAL4^unspecified does not suppress the eye overgrowth seen in sav^SH13 mutant eye clones.

(Huang et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington