FlyBase Allele Report: Dmel\Pcf11[HMS00406]

General Information

Symbol

Dmel\Pcf11^HMS00406

Species

D. melanogaster

Name

FlyBase ID

FBal0248167

Feature type

allele

Associated gene

Dmel\Pcf11

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00406}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00406}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00406

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

autophagosome | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Tang et al., 2018)

embryonic/larval fat body | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Tang et al., 2018)

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

extracellular matrix | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

(Ke et al., 2018)

Detailed Description

Statement

Reference

The expression of Pcf11^HMS00406 under the control of Scer\GAL4^{SPARC-MI00329-GAL4} (and Dicer-2, for a more efficient RNAi) in the larval fat body induces defective secretion, including intracellular matrix material, as shown by the retention of both secr.GFP and Vkg.GFP.

(Ke et al., 2018)

Under standard nutritional conditions, larval fat body clones expressing Pcf11^HMS00406 under the control of Scer\GAL4^Act.PU show a significant increase in autophagosomes (identified as mCherry-Atg8 puncta).

(Tang et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Pcf11^HMS00406, Scer\GAL4^GMR.PU is an enhancer of abnormal size | adult stage phenotype of Atg1^UAS.cSa, Scer\GAL4^GMR.PU

(Tang et al., 2018)

Pcf11^HMS00406, Scer\GAL4^GMR.PU is an enhancer of visible | adult stage phenotype of Atg1^UAS.cSa, Scer\GAL4^GMR.PU