FlyBase Allele Report: Dmel\Sirt1[HMS00484]

General Information

Symbol

Dmel\Sirt1^HMS00484

Species

D. melanogaster

Name

FlyBase ID

FBal0248236

Feature type

allele

Associated gene

Dmel\Sirt1

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS00484}

Also Known As

sir2 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS00484}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS00484

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Larnerd et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant | adult stage, with Scer\GAL4^elav-C155

(Engel et al., 2016)

visible | adult stage, with Scer\GAL4^en.PU

(Dietz et al., 2015)

visible | adult stage, with Scer\GAL4^GMR.PF

(Dietz et al., 2015)

visible | adult stage, with Scer\GAL4^nub.PK

(Dietz et al., 2015)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Dietz et al., 2015)

wing vein, with Scer\GAL4^en.PU

(Dietz et al., 2015)

wing vein, with Scer\GAL4^nub.PK

(Dietz et al., 2015)

Detailed Description

Statement

Reference

Expression of Sirt1^HMS00484 under the control of Scer\GAL4^elav-C155 results in significantly decreased sedation sensitivity to acute ethanol exposure in naive flies, and a significant decrease in sedation tolerance when repeatedly exposed to ethanol, as compared to controls.

(Engel et al., 2016)

Driving expression of Sirt1^HMS00484 in the fat body (using Scer\GAL4^r4) but not elsewhere (Scer\GAL4^Mef2.PR, Scer\GAL4^mex1.2.1, Scer\GAL4^Ilp2.215-1, Scer\GAL4^Akh.PL) disrupts insulin signaling and leads to hyperglycemia compared to controls (at 2 weeks old).

(Palu and Thummel, 2016)

Expression of Sirt1^HMS00484 results in: slightly rough eye phenotype (with Scer\GAL4^GMR.PF), occasional ectopic vein material in the anterior wing or at the posterior crossvein (with Scer\GAL4^nub.PK) or in the posterior wing compartment (with Scer\GAL4^en.PU). Scer\GAL4^Act5C.PI-driven expression is viable; no overt adult phenotype is observed with the Scer\GAL4^ey.PH driver.

(Dietz et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions