FB2025_01 , released February 20, 2025
Allele: Mmus\Cd8aIVS.10xUAS.GFP
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General Information
Symbol
Mmus\Cd8aIVS.10xUAS.GFP
Species
M. musculus
Name
FlyBase ID
FBal0248274
Feature type
allele
Associated gene
Mmus\Cd8a
Associated Insertion(s)
Carried in Construct
P{10XUAS-IVS-mCD8::GFP.TH1}
P{10XUAS-IVS-mCD8::GFP.THS2}
P{10XUAS-IVS-mCD8::GFP}
Also Known As
UAS-mCD8::GFP, pJFRC2-10XUAS-IVS-mCD8::GFP, 10XUAS-IVS-mCD8::GFP, UAS-CD8-GFP, pJFRC2, UAS-mCD8-GFP, 10xUAS-ivs-mCD8-gfp, 10XUAS-mCD8::GFP, UAS-IVS-mCD8::GFP, UAS-GFP
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Pfeiffer et al., 2010)
Progenitor genotype
Carried in construct
P{10XUAS-IVS-mCD8::GFP.TH1}
P{10XUAS-IVS-mCD8::GFP.THS2}
P{10XUAS-IVS-mCD8::GFP.TTS3}
P{10XUAS-IVS-mCD8::GFP}
PBac{10XUAS-IVS-mCD8::GFP}
Cytology
Description

Ten copies of the UAS site followed by an Hsp70 basal promoter are fused upstream of a Mmus\Cd8a-Avic\GFP fusion protein. A 67bp intron derived from Mhc has been inserted in the 5' UTR. An SV40 transcriptional terminator is present.

(Pfeiffer et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Mmus\Cd8a
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (6)
      Notes on Origin
      Discoverer
      Comments
      Comments

      The P{10XUAS-IVS-mCD8::GFP} construct contains a single copy of Mmus\Cd8aIVS.10xScer\UAS.T:Avic\GFP.

      (Pfeiffer et al., 2010)

      The P{10XUAS-IVS-mCD8::GFP.TH1} construct contains two copies of Mmus\Cd8aIVS.10xScer\UAS.T:Avic\GFP in the same (tail-to-head) orientation.

      (Pfeiffer et al., 2010)

      The P{10XUAS-IVS-mCD8::GFP.THS2} construct contains two copies of Mmus\Cd8aIVS.10xScer\UAS.T:Avic\GFP in the same (tail-to-head) orientation, separated by a 2.8kb gypsy-insulated spacer.

      (Pfeiffer et al., 2010)

      The P{10XUAS-IVS-mCD8::GFP.TTS3} construct contains two copies of Mmus\Cd8aIVS.10xScer\UAS.T:Avic\GFP in tail-to-tail orientation, separated by a 2.8kb gypsy-insulated spacer.

      (Pfeiffer et al., 2010)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Mmus\Cd8aIVS.10xScer\UAS.T:Avic\GFP
      Mmus\Cd8aIVS.10xUAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (61)