FlyBase Allele Report: Dmel\Pgam5[NP0568]

General Information

Symbol

Dmel\Pgam5^NP0568

Species

D. melanogaster

Name

FlyBase ID

FBal0248709

Feature type

allele

Associated gene

Dmel\Pgam5

Associated Insertion(s)

P{GawB}Pgam5^NP0568

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Imai et al., 2010)

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GawB}Pgam5^NP0568

Cytology

Description

Insertion 50bp downstream of the translation start site.

(Imai et al., 2010)

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

X:1,873,489..1,873,660 [+]

(Olson et al., 2019, Imai et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Pink1^B9

(Imai et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

mitochondrion

(Imai et al., 2010)

Detailed Description

Statement

Reference

The morphology of the mitochondria in the indirect flight muscle of 2 day old mutant adults appears to be slightly altered compared to wild type; the mitochondria are longer in the long-axis direction than normal and constrictions are often seen in the mitochondria.

(Imai et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Pgam5^NP0568 is a suppressor of abnormal locomotor behavior phenotype of Pink1^B9

(Imai et al., 2010)

Pgam5^NP0568 is a suppressor of visible phenotype of Pink1^B9

(Imai et al., 2010)

Pgam5^NP0568 is a suppressor of short lived phenotype of Pink1^B9

(Imai et al., 2010)

Pgam5^NP0568 is a suppressor | partially of visible phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Imai et al., 2010)

NOT Suppressor of

Statement

Reference

Pgam5^NP0568 is a non-suppressor of short lived phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Imai et al., 2010)

Pgam5^NP0568 is a non-suppressor of visible phenotype of park^P21

(Imai et al., 2010)

Pgam5^NP0568 is a non-suppressor of abnormal locomotor behavior phenotype of park^P21

(Imai et al., 2010)

Pgam5^NP0568 is a non-suppressor of short lived phenotype of park^P21

(Imai et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

Pgam5^NP0568 is a suppressor of dopaminergic PPL1 neuron phenotype of Pink1^B9

(Imai et al., 2010)

Pgam5^NP0568 is a suppressor of dopaminergic PPM1 neuron phenotype of Pink1^B9

(Imai et al., 2010)

Pgam5^NP0568 is a suppressor of dopaminergic PPM2 neuron phenotype of Pink1^B9

(Imai et al., 2010)

Pgam5^NP0568 is a suppressor of mitochondrion phenotype of park^P21

(Imai et al., 2010)

Pgam5^NP0568 is a suppressor | partially of wing phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Imai et al., 2010)

Pgam5^NP0568 is a suppressor of adult thorax phenotype of Pink1^B9

(Imai et al., 2010)

Pgam5^NP0568 is a suppressor of wing phenotype of Pink1^B9

(Imai et al., 2010)

Pgam5^NP0568 is a suppressor | partially of mitochondrion phenotype of Pink1^B9

(Imai et al., 2010)

NOT Suppressor of

Statement

Reference

Pgam5^NP0568 is a non-suppressor of wing phenotype of park^P21

(Imai et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Pgam5^NP0568 significantly suppresses the abnormal wing posture of flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PW, but fails to suppress the short lifespan phenotype of these flies.

The defects in the adult thorax, the progressive abnormal wing posture phenotype, the progressive loss of climbing ability and the shorter lifespan seen in Pink1^B9 flies are suppressed if they are also mutant for Pgam5^NP0568.

The mitochondrial hyperfusion and loss of cristae of the mitochondria of indirect flight muscles of one day old Pink1^B9 adults is partially suppressed by Pgam5^NP0568.

The loss of dopaminergic neurons in the protocerebral posterior lateral 1 (PPL1) and protocerebral posterior medial 1 and 2 (PPM1/2) clusters of the brain that is seen in 25 day old Pink1^B9 adults is suppressed if the flies are also mutant for Pgam5^NP0568.

Pgam5^NP0568 has little effect on the abnormal wing posture phenotype of park^P21 flies, and fails to rescue the progressive loss in climbing ability and reduced lifespan of park^P21 flies.

The elongated morphology of the mitochondria in the indirect flight muscles of park^P21 adults is suppressed by Pgam5^NP0568.

(Imai et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

103655

y^* P{GawB}Pgam5^NP0568 w^* / FM7c

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

PGAM5^NP0568

(Imai et al., 2010)

Pgam5^NP0568

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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