FlyBase Allele Report: Dmel\Pgam5[1]

General Information

Symbol

Dmel\Pgam5¹

Species

D. melanogaster

Name

FlyBase ID

FBal0248710

Feature type

allele

Associated gene

Dmel\Pgam5

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Imai et al., 2010)

Mutagen

Delta2-3 transposase

(Imai et al., 2010)

Progenitor genotype

P{SUPor-P}KG09727

(Imai et al., 2010)

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in a deletion of the entire Pgam5 coding region.

(Imai et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Pink1^B9

(Imai et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal heat stress response

(Ishida et al., 2012)

abnormal locomotor behavior | adult stage

(Ishida et al., 2012)

decreased cell number | adult stage

(Ishida et al., 2012)

fertile

(Imai et al., 2010)

increased cell death | adult stage

(Ishida et al., 2012)

long lived

(Imai et al., 2010)

viable

(Imai et al., 2010)

Phenotype Manifest In

adult mushroom body

(Ishida et al., 2012)

Kenyon cell

(Ishida et al., 2012)

mitochondrion

(Imai et al., 2010)

Detailed Description

Statement

Reference

Pgam5¹ mutant flies have a similar lifespan to wild-type flies when maintained on a glucose-based diet.

Pgam5¹ flies show neither resistance or sensitivity to oxidative stress (H[[2]]O[[2]]) and starvation, but are sensitive to heat shock stress and die sooner than control flies.

No morphological changes are seen in the mushroom bodies of Pgam5¹ mutant flies when Mmus\Cd8a^{Scer\UAS.T:Avic\GFP} is expressed under the control of Scer\GAL4^c739. However expressing His2B^{Scer\UAS.T:Avic\GFP-ECFP} under the control of Scer\GAL4^c739 shows a reduction in the number of Kenyon Cell nuclei following heat shock treatment compared to control flies. An increased number of TUNEL positive apoptotic cells are seen in the mushroom bodies of Pgam5¹ mutant flies but no difference is seen in the amount of apoptosis in other brain regions, such as the optic lobe.

Pgam5¹ mutant flies in which the mushroom bodies have been ablated by treatment with hydroxyurea at the larval stage are more resistant to heat stress than those in which the mushroom bodies are present.

Pgam5¹ mutant flies have reduced locomotor activity compared to wild type, both under normal conditions and in response to heat shock stress. Wild type flies have a peak in activity 30 minutes after stress and activity levels fall over the next 60 minutes, whereas activity levels in Pgam5¹ mutant flies peak immediately after heat stress before falling over the following 60 minutes.

(Ishida et al., 2012)

Homozygotes appear grossly normal, but have a longer lifespan than controls.

Mitochondria in the indirect flight muscles are longer in the long-axis direction than normal in 5 day old mutant adults.

(Imai et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pgam5¹ has abnormal heat stress response phenotype, enhanceable by Pink1^B9

(Ishida et al., 2012)

Pgam5¹ has abnormal heat stress response phenotype, enhanceable by shi^1.UAS/Scer\GAL4^c739

(Ishida et al., 2012)

Suppressed by

Statement

Reference

Pgam5¹ has abnormal heat stress response phenotype, suppressible by BacA\p35^UAS.cHa/Scer\GAL4^c739

(Ishida et al., 2012)

Enhancer of

Statement

Reference

Pgam5¹ is an enhancer of abnormal heat stress response phenotype of Pink1^B9

(Ishida et al., 2012)

Suppressor of

Statement

Reference

Pgam5¹ is a suppressor of short lived phenotype of Pink1^B9

(Imai et al., 2010)

Pgam5¹ is a suppressor of abnormal locomotor behavior phenotype of Pink1^B9

(Imai et al., 2010)

Pgam5¹ is a suppressor of visible phenotype of Pink1^B9

(Imai et al., 2010)

Phenotype Manifest In

Suppressor of

Statement

Reference

Pgam5¹ is a suppressor of adult thorax phenotype of Pink1^B9

(Imai et al., 2010)

Pgam5¹ is a suppressor of wing phenotype of Pink1^B9

(Imai et al., 2010)

Pgam5¹ is a suppressor | partially of mitochondrion phenotype of Pink1^B9

(Imai et al., 2010)

Pgam5¹ is a suppressor of dopaminergic PPL1 neuron phenotype of Pink1^B9

(Imai et al., 2010)

Pgam5¹ is a suppressor of dopaminergic PPM1 neuron phenotype of Pink1^B9

(Imai et al., 2010)

Pgam5¹ is a suppressor of dopaminergic PPM2 neuron phenotype of Pink1^B9

(Imai et al., 2010)

NOT Suppressor of

Statement

Reference

Pgam5¹ is a non-suppressor of mitochondrion phenotype of Marf^GD11094, Scer\GAL4^Mhc.PW

(Imai et al., 2010)

Pgam5¹ is a non-suppressor of mitochondrion phenotype of Marf^KK105681, Scer\GAL4^Mhc.PW

(Imai et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Pgam5¹ Pink1^B9 double mutant flies are more sensitive to heat shock stress than either single mutant alone.

Expression of shi^1.Scer\UAS under the control of Scer\GAL4^c739 enhances the sensitivity to heat stress seen in Pgam5¹ mutant flies.

(Ishida et al., 2012)

Pgam5¹ does not suppress the abnormal mitochondrial phenotype (mitochondria in the indirect flight muscles are shorter in the long-axis direction than normal) seen in 5 day old adults expressing either Marf^GD11094 or Marf^KK105681 under the control of Scer\GAL4^Mhc.PW.

The defects in the adult thorax, the progressive abnormal wing posture phenotype, the progressive loss of climbing ability and the shorter lifespan seen in Pink1^B9 flies are suppressed if they are also mutant for Pgam5¹.

The mitochondrial hyperfusion and loss of cristae of the mitochondria of indirect flight muscles of one day old Pink1^B9 adults is partially suppressed by Pgam5¹.

The loss of dopaminergic neurons in the protocerebral posterior lateral 1 (PPL1) and protocerebral posterior medial 1 and 2 (PPM1/2) clusters of the brain that is seen in 25 day old Pink1^B9 adults is suppressed if the flies are also mutant for Pgam5¹.

(Imai et al., 2010)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa under the control of Scer\GAL4^c739 suppresses the heat shock stress sensitivity see in Pgam5¹ mutant flies.

(Ishida et al., 2012)

Complementation and Rescue Data

Rescued by

Pgam5¹ is rescued by Pgam5^UAS.cIa/Scer\GAL4^c739

(Ishida et al., 2012)

Not rescued by

Pgam5¹ is not rescued by Pgam5^H94A.UAS/Scer\GAL4^c739

(Ishida et al., 2012)

Comments

Expression of Pgam5^Scer\UAS.cIa in mushroom bodies under the control of Scer\GAL4^c739 rescues the heat shock sensitivity seen in Pgam5¹ mutant flies.

Expression of Pgam5^{H94A.Scer\UAS} in mushroom bodies under the control of Scer\GAL4^c739 fails to rescue the heat shock sensitivity seen in Pgam5¹ mutant flies.

(Ishida et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

PGAM5¹

(Ishida et al., 2012, Imai et al., 2010)

Pgam5¹

Name Synonyms

Secondary FlyBase IDs

References (2)

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