FlyBase Allele Report: Dmel\Mst36Fb[RNAi.Sym.UAS]

General Information

Symbol

Dmel\Mst36Fb^RNAi.Sym.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0248741

Feature type

allele

Associated gene

Dmel\Mst36Fb

Associated Insertion(s)

Carried in Construct

P{Sym-UAS-Mst36Fb.2934}

Key Links

Transgenic product class

RNAi_reagent

(Di Cara et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Di Cara et al., 2010)

Mutagen

in vitro construct

(Di Cara et al., 2010)

Progenitor genotype

Carried in construct

P{Sym-UAS-Mst36Fb.2934}

Cytology

Description

Sense-antisense transcription (driven by two convergent arrays of UAS sequence) of a 2934 bp fragment corresponding to 1146 bp upstream of the Mst36Fb transcription start site and the first 1788 bp of Mst36Fb coding sequence results in double stranded RNA (dsRNA) expression.

(Di Cara et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mst36Fb

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male semi-fertile, with Scer\GAL4^aly.PU

(Di Cara et al., 2010)

male semi-fertile, with Scer\GAL4^da.G32

(Di Cara et al., 2010)

male semi-fertile, with Scer\GAL4^nanos.PG

(Di Cara et al., 2010)

male semi-fertile, with Scer\GAL4^VP16.bam

(Di Cara et al., 2010)

male semi-fertile, with Scer\GAL4^αTub84B.PL

(Di Cara et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Mst36Fb^{Sym.2934.Scer\UAS} and Mst36Fb^{a.Sym.2934.Scer\UAS} in the germline under the control of Scer\GAL4^nos.PG silences Mst36Fb to an extent that it significantly reduces fertility, with fecundity reduced by 30%. The presence of two copies of Scer\GAL4^nos.PG increases penetrance, with a 50% reduction in male fertility.

Expression of Mst36Fb^{Sym.2934.Scer\UAS} and Mst36Fb^{a.Sym.2934.Scer\UAS} in the germline under the control of Scer\GAL4^da.G32 silences Mst36Fb to an extent that it significantly reduces fertility, with fecundity reduced by 35%.

Expression of Mst36Fb^{Sym.2934.Scer\UAS} and Mst36Fb^{a.Sym.2934.Scer\UAS} in the germline under the control of Scer\GAL4^{bam.T:Hsim\VP16} silences Mst36Fb to an extent that it significantly reduces fertility, with fecundity reduced by 20%.

Expression of Mst36Fb^{Sym.2934.Scer\UAS} and Mst36Fb^{a.Sym.2934.Scer\UAS} in the germline under the control of Scer\GAL4^aly silences Mst36Fb to an extent that it significantly reduces fertility, with fecundity reduced by 20%.

Expression of Mst36Fb^{Sym.2934.Scer\UAS} and Mst36Fb^{a.Sym.2934.Scer\UAS} in the germline under the control of Scer\GAL4^αTub84B.PL silences Mst36Fb to an extent that it significantly reduces fertility, with fecundity reduced by 25%.

Expression of Mst36Fb^{dsRNA.Sym.Scer\UAS} in the germline under the control of Scer\GAL4^nos.PG silences Mst36Fb to an extent that it significantly reduces fertility, with fecundity reduced by 30%. The presence of two copies of Scer\GAL4^nos.PG increases penetrance, with a 50% reduction in male fertility.

Expression of Mst36Fb^{dsRNA.Sym.Scer\UAS} in the germline under the control of Scer\GAL4^da.G32 silences Mst36Fb to an extent that it significantly reduces fertility, with fecundity reduced by 35%.

Expression of Mst36Fb^{dsRNA.Sym.Scer\UAS} in the germline under the control of Scer\GAL4^{bam.T:Hsim\VP16} silences Mst36Fb to an extent that it significantly reduces fertility, with fecundity reduced by 20%.

Expression of Mst36Fb^{dsRNA.Sym.Scer\UAS} in the germline under the control of Scer\GAL4^aly silences Mst36Fb to an extent that it significantly reduces fertility, with fecundity reduced by 20%.

Expression of Mst36Fb^{dsRNA.Sym.Scer\UAS} in the germline under the control of Scer\GAL4^αTub84B.PL silences Mst36Fb to an extent that it significantly reduces fertility, with fecundity reduced by 25%.

(Di Cara et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Mst36Fa^{dsRNA.Sym.Scer\UAS} and Mst36Fb^{dsRNA.Sym.Scer\UAS} under the control of two copies of Scer\GAL4^nos.PG results in a smaller male fertility reduction than in 'single' mutants, perhaps indicating a quenching effect of the Scer\GAL4^nos.PG on both constructs.

Expression of Mst36Fa^{Sym.2960.Scer\UAS} and Mst36Fa^{a.Sym.2960.Scer\UAS} for Mst36Fa and Mst36Fb^{Sym.2934.Scer\UAS} and Mst36Fb^{a.Sym.2934.Scer\UAS} for Mst36Fb, all under the control of two copies of Scer\GAL4^nos.PG results in a smaller male fertility reduction than in 'single' mutants, perhaps indicating a quenching effect of the Scer\GAL4^nos.PG on both constructs.

(Di Cara et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Associated with: Mst36Fb^{a.Sym.2934.Scer\UAS}.

(Di Cara et al., 2010)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Mst36Fb^RNAi.Sym.UAS

Mst36Fb^{Sym.2934.Scer\UAS}

Mst36Fb^{dsRNA.Sym.Scer\UAS}

Mst36Fb^{dsRNA.Sym.UAS}

Name Synonyms

Secondary FlyBase IDs

FBal0248742

References (2)

Report Sections

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