FB2025_01 , released February 20, 2025
Allele: Dmel\Cyp18a1UAS.cGa
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General Information
Symbol
Dmel\Cyp18a1UAS.cGa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Guittard
FlyBase ID
FBal0248788
Feature type
allele
Associated gene
Dmel\Cyp18a1
Associated Insertion(s)
Carried in Construct
P{UAS-Cyp18a1.G}
Also Known As
UAS-Cyp18a1
Key Links
Transgenic product class
wild_type
(Guittard et al., 2011)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Guittard et al., 2011)
Mutagen
in vitro construct
(Guittard et al., 2011)
Progenitor genotype
Carried in construct
P{UAS-Cyp18a1.G}
Cytology
Description

UAS regulatory sequences drive expression of Cyp18a1.

(Guittard et al., 2011)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Cyp18a1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      lethal | P-stage, with Scer\GAL4Cyp6g1.HR

      (Guittard et al., 2011)

      lethal | third instar larval stage, with Scer\GAL4Cyp6g1.HR

      (Guittard et al., 2011)

      lethal - all die before end of larval stage, with Scer\GAL4Cyp6g1.HR

      (Scanlan and Robin, 2024)

      lethal - all die before end of larval stage, with Scer\GAL4MsrA.657

      (Scanlan and Robin, 2024)

      lethal - all die before end of P-stage, with Scer\GAL4Cg.PA

      (Scanlan and Robin, 2024)

      lethal - all die before end of P-stage, with Scer\GAL4dan-AC116

      (Scanlan and Robin, 2024)

      lethal - all die before end of P-stage, with Scer\GAL4Desat1.PB

      (Scanlan and Robin, 2024)

      lethal - all die before end of P-stage, with Scer\GAL4mex1.2.1

      (Scanlan and Robin, 2024)

      lethal - all die during embryonic stage, with Scer\GAL4Act.PU

      (Scanlan and Robin, 2024)

      lethal - all die during embryonic stage, with Scer\GAL4btl.PS

      (Scanlan and Robin, 2024)

      lethal - all die during embryonic stage, with Scer\GAL4c204

      (Scanlan and Robin, 2024)

      lethal - all die during embryonic stage, with Scer\GAL4da.PU

      (Scanlan and Robin, 2024)

      lethal - all die during embryonic stage, with Scer\GAL4elav-C155

      (Scanlan and Robin, 2024)

      lethal - all die during embryonic stage, with Scer\GAL4Mef2.PR

      (Scanlan and Robin, 2024)

      lethal - all die during embryonic stage, with Scer\GAL4nSyb.PS

      (Scanlan and Robin, 2024)

      lethal - all die during embryonic stage, with Scer\GAL4phtm.PG

      (Guittard et al., 2011)

      lethal - all die during embryonic stage, with Scer\GAL4phtm.PU

      (Scanlan and Robin, 2024)

      lethal - all die during embryonic stage, with Scer\GAL4ppl.PP

      (Scanlan and Robin, 2024)

      lethal - all die during embryonic stage, with Scer\GAL4Tub.PU

      (Scanlan and Robin, 2024, Guittard et al., 2011)

      melanotic mass phenotype | third instar larval stage, with Scer\GAL4Cyp6g1.HR

      (Guittard et al., 2011)

      partially lethal - majority live, with Scer\GAL4Sgs3.PD

      (Scanlan and Robin, 2024)

      some die during embryonic stage, with Scer\GAL4phtm.PG

      (Guittard et al., 2011)

      some die during embryonic stage, with Scer\GAL4Tub.PU

      (Guittard et al., 2011)

      some die during P-stage, with Scer\GAL4Sgs3.PD

      (Scanlan and Robin, 2024)

      viable, with Scer\GAL4e22c

      (Scanlan and Robin, 2024)

      viable, with Scer\GAL4Uro.PT

      (Scanlan and Robin, 2024)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Overexpression of Cyp18a1Scer\UAS.cGa driven by Scer\GAL4Cyp6g1.HR in the larval midgut, Malpighian tubules and fat body results primarily in third instar larval lethality, with a number of individuals surviving until early pupal stages. Melanotic masses can be seen in many larvae, often associated with the midgut and cuticle.

      Ubiquitous overexpression of Cyp18a1null644 driven by Scer\GAL4tub.PU results in late embryonic lethality. This embryonic lethality can be rescued to first instar larval stage by incubating embryos in 20-hydroxyecdysone (20E).

      Overexpression of Cyp18a1Scer\UAS.cGa driven by Scer\GAL4phm.PG in the prothoracic cells of the ring gland results in late embryonic lethality. This embryonic lethality can be rescued to first instar larval stage by incubating embryos in 20-hydroxyecdysone (20E).

      (Guittard et al., 2011)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Cyp18a1Scer\UAS.cGa
      Cyp18a1UAS.cGa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Guittard
      Secondary FlyBase IDs
        References (2)