Allele Dmel\Cyp18a1^Scer\UAS.cGa

General Information
Symbol	Dmel\Cyp18a1^Scer\UAS.cGa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Guittard	FlyBase ID	FBal0248788
Feature type	allele	Associated gene	Dmel\Cyp18a1

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Guittard et al., 2011)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of Cyp18a1. (Guittard et al., 2011)
Carried in construct	P{UAS-Cyp18a1.G} (Guittard et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| late embryonic stage, with Scer\GAL4^phm.PG (Guittard et al., 2011) lethal \| late embryonic stage, with Scer\GAL4^tub.PU (Guittard et al., 2011) lethal \| P-stage, with Scer\GAL4^Cyp6g1.HR (Guittard et al., 2011) lethal \| third instar larval stage, with Scer\GAL4^Cyp6g1.HR (Guittard et al., 2011) melanotic mass phenotype \| third instar larval stage, with Scer\GAL4^Cyp6g1.HR (Guittard et al., 2011)
Phenotype Manifest In
	cuticle \| third instar larval stage, with Scer\GAL4^Cyp6g1.HR (Guittard et al., 2011) midgut \| third instar larval stage, with Scer\GAL4^Cyp6g1.HR (Guittard et al., 2011)
Detailed Description
	Statement Reference Overexpression of Cyp18a1[Scer\UAS.cGa] driven by Scer\GAL4[Cyp6g1.HR] in the larval midgut, Malpighian tubules and fat body results primarily in third instar larval lethality, with a number of individuals surviving until early pupal stages. Melanotic masses can be seen in many larvae, often associated with the midgut and cuticle. Ubiquitous overexpression of Cyp18a1[null644] driven by Scer\GAL4[tub.PU] results in late embryonic lethality. This embryonic lethality can be rescued to first instar larval stage by incubating embryos in 20-hydroxyecdysone (20E). Overexpression of Cyp18a1[Scer\UAS.cGa] driven by Scer\GAL4[phm.PG] in the prothoracic cells of the ring gland results in late embryonic lethality. This embryonic lethality can be rescued to first instar larval stage by incubating embryos in 20-hydroxyecdysone (20E). (Guittard et al., 2011)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Cyp18a1^Scer\UAS.cGa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Guittard
Secondary FlyBase IDs

References ( 1 )
Research paper	Guittard et al., 2011, Dev. Biol. 349(1): 35--45 CYP18A1, a key enzyme of Drosophila steroid hormone inactivation, is essential for metamorphosis. [FBrf0212396]

Allele Dmel\Cyp18a1Scer\UAS.cGa

Allele Dmel\Cyp18a1^Scer\UAS.cGa