Allele Dmel\Ras85D^{12V,C186S.Scer\UAS.T:Hsap\MYC}

General Information
Symbol	Dmel\Ras85D^{12V,C186S.Scer\UAS.T:Hsap\MYC}	Species	D. melanogaster
Name		FlyBase ID	FBal0248800
Feature type	allele	Associated gene	Dmel\Ras85D

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct - site directed mutagenesis, in vitro construct - coding region fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - coding region fusion (Sung et al., 2010) in vitro construct - regulatory fusion (Sung et al., 2010) in vitro construct - site directed mutagenesis (Sung et al., 2010)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Scer\UAS regulatory sequences drive expression of an activated form of Ras85D (12V), carrying the mutation C186S to inactivate membrane targeting, tagged at with 6 copies of T:Hsap\MYC. (Sung et al., 2010)
Carried in construct	P{UAS-Ras1.12V,C186S} (Sung et al., 2010)
Tagged with	T:Hsap\MYC (Sung et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	Ras85D^{12V,C186S.Scer\UAS.T:Hsap\MYC}, Scer\GAL80^ts.αTub84B, Scer\GAL4^αTub84B.PL rescues Ras85D^ΔC40B (Sung et al., 2010)
Comments	Expression of Ras85D[12V,C186S.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[αTub84B.PL] in combination with Scer\GAL80[ts.αTub84B] in eye disc clones rescues the ommatidial development defects of Ras85D[ΔC40B] homozygous cells. Clones show an enhanced recruitment of supernumerary photoreceptor cells. (Sung et al., 2010)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Ras85D^{12V,C186S.Scer\UAS.T:Hsap\MYC}
Name Synonym
Secondary FlyBase IDs

References ( 1 )
Research paper	Sung et al., 2010, Mol. Cell. Biol. 30(24): 5649--5657 Cytosolic ras supports eye development in Drosophila. [FBrf0212407]

Allele Dmel\Ras85D12V,C186S.Scer\UAS.T:Hsap\MYC

Allele Dmel\Ras85D^{12V,C186S.Scer\UAS.T:Hsap\MYC}